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Method for improving fermentation unit of erythromycin

A technology of erythromycin and unit, which is applied in the field of biology and new medicine, can solve the problems of increasing raw material and energy consumption, high extraction cost, and long fermentation cycle, so as to promote the growth and reproduction of bacteria, improve the level of fermentation technology, and shorten the fermentation period. cycle effect

Pending Publication Date: 2020-02-04
NINGXIA QIYUAN PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the prior art, in the process of fermenting and producing erythromycin with Saccharopolyspora rubrum as the starting strain, traditional starch, nitrogen sources, inorganic salts, etc. are used as base materials, and a large amount of late-acting nitrogen sources and phosphorus are added throughout the process. This method has the following technical disadvantages: 1) long fermentation period; 2) low fermentation unit; 3) long fermentation process, long feeding time, easy to infect bacteria; 4) high energy and material consumption
Due to the long fermentation cycle, a lot of materials are added during the process, and there are many residual proteins and solids in the fermentation broth, which increases the consumption of raw materials and energy, and the extraction cost is high.

Method used

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  • Method for improving fermentation unit of erythromycin
  • Method for improving fermentation unit of erythromycin
  • Method for improving fermentation unit of erythromycin

Examples

Experimental program
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Effect test

Embodiment 1

[0032] 1) Example fermentation medium composition: starch 580g / L, dextrin 150g / L, soybean powder 375g / L, corn steep liquor 125g / L, ammonium sulfate 40g / L, sodium chloride 37.5g / L, calcium carbonate 105g / L L, soybean oil 45g / L, nickel sulfate 0.03g / L.

[0033] 2) The medium composition of the control example: starch 580g / L, dextrin 150g / L, soybean flour 525g / L, corn steep liquor 300g / L, ammonium sulfate 40g / L, sodium chloride 37.5g / L, calcium carbonate 105g / L , soybean oil 50g / L.

[0034] 3) Prepare and cultivate seeds of all levels according to the above-mentioned requirements, including inclined spores, first-level seeds, and second-level seeds.

[0035] 4) Inoculate the secondary seed liquid cultivated in the above method into the sterilized fermentation medium of the embodiment and the fermentation medium of the control example under aseptic conditions, and cultivate according to the normal fermentation culture conditions. Hours, 100 hours, 150 hours to monitor the fermen...

Embodiment 2

[0038] 1) Example fermentation medium composition: starch 580g / L, dextrin 150g / L, soybean powder 375g / L, corn steep liquor 125g / L, ammonium sulfate 40g / L, sodium chloride 37.5g / L, calcium carbonate 105g / L L, soybean oil 45g / L, nickel sulfate 0.05g / L.

[0039] 2) The medium composition of the control example: starch 580g / L, dextrin 150g / L, soybean flour 525g / L, corn steep liquor 300g / L, ammonium sulfate 40g / L, sodium chloride 37.5g / L, calcium carbonate 105g / L , soybean oil 50g / L.

[0040] 3) Prepare and cultivate seeds of all levels according to the above-mentioned requirements, including inclined spores, first-level seeds, and second-level seeds.

[0041] 4) Inoculate the secondary seed liquid cultivated in the above method into the sterilized fermentation medium of the embodiment and the fermentation medium of the control example under aseptic conditions, and cultivate according to the normal fermentation culture conditions, and ferment for 50 hours respectively , 100 hours...

Embodiment 3

[0044]1) Example fermentation medium composition: starch 580g / L, dextrin 150g / L, soybean powder 375g / L, corn steep liquor 125g / L, ammonium sulfate 40g / L, sodium chloride 37.5g / L, calcium carbonate 105g / L L, soybean oil 45g / L, nickel sulfate 0.06g / L.

[0045] 2) The medium composition of the control example: starch 580g / L, dextrin 150g / L, soybean flour 525g / L, corn steep liquor 300g / L, ammonium sulfate 40g / L, sodium chloride 37.5g / L, calcium carbonate 105g / L , soybean oil 50g / L.

[0046] 3) Prepare and cultivate seeds of all levels according to the above-mentioned requirements, including inclined spores, first-level seeds, and second-level seeds.

[0047] 4) Inoculate the secondary seed liquid cultivated in the above method into the sterilized fermentation medium of the embodiment and the fermentation medium of the control example under aseptic conditions, and cultivate according to the normal fermentation culture conditions, and ferment for 50 hours respectively , 100 hours,...

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Abstract

The invention relates to a method for improving a fermentation unit of erythromycin. The method is characterized in that in the process of taking saccharopolyspora erythraea as an original strain to produce the erythromycin by fermentation, nickel sulfate is added into a fermentation medium at the use amount of 0.03-0.06 g / L. According to the method, in the process of taking the saccharopolysporaerythraea as the original strain to produce the erythromycin by fermentation, an appropriate amount of the nickel sulfate is added into the fermentation medium of the erythromycin, the nickel sulfatecan participate in the processes of the growth and reproduction of bacterial cells and the synthesis of products, the nickel sulfate is used for stimulating the enzyme activity of the metabolic process, the growth and reproduction of bacteria are promoted, and more importantly, the synthesis rate of the products is promoted, so as to achieve the purposes of shortening the fermentation period, improving the fermentation technological level, and meanwhile reducing consumption.

Description

technical field [0001] The invention belongs to biological and new medical technology, in particular to a method for increasing the fermentation unit of erythromycin. Background technique [0002] Erythromycin is a macrolide antibiotic with an antimicrobial spectrum similar to that of penicillin. It is often used clinically for the treatment of patients allergic to penicillin. In recent years, due to the extensive development and use of erythromycin derivatives, the demand for erythromycin continues to increase. [0003] In the prior art, in the process of fermenting and producing erythromycin with Saccharopolyspora rubrum as the starting strain, traditional starch, nitrogen sources, inorganic salts, etc. are used as base materials, and a large amount of late-acting nitrogen sources and phosphorus are added throughout the process. This method has the following technical disadvantages: 1) long fermentation period; 2) low fermentation unit; 3) long fermentation process, long ...

Claims

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Application Information

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IPC IPC(8): C12P19/62C12N1/20C12N1/38C12R1/01
CPCC12P19/62C12N1/20C12N1/38
Inventor 章惠霞李学兵张志
Owner NINGXIA QIYUAN PHARMA
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