Hetero-dimeric multi-specific antibody format targeting at least cd3 and hsa

Abstract

This invention relates to novel hetero-dimeric multi-specific format of multiple antibody variable domains comprising a core of two split variable domain pairs wherein both variable light domains andthe two cognate variable heavy domains are positioned in tandem on two separate protein chains, respectively.

Description

technical field [0001] The present invention relates to novel heterodimeric multispecific forms of multiple antibody variable domains comprising a core of two separate variable domain pairs, wherein two variable light domains and two homologous The variable heavy domains are located in tandem on two separate protein chains. Background technique [0002] In the past forty years since the development of the first monoclonal antibody [R17], antibodies have become an increasingly important class of biomolecules for research, diagnostic and therapeutic purposes. [0003] Antibodies as therapeutics are moving towards more rationally engineered functions that improve and expand their intrinsic properties. Examples include optimization of effector function by glycoengineering [R18], specific localization such as transfer across the blood-brain barrier [R19] or modulation of half-life by, for example, increased binding to FcRn. [0004] A complementary approach to antibody function...

AI Technical Summary

Problems solved by technology

[0024] A solution to this problem, namely the identification of a defined core of two fixed variable domain pairs (which can be achieved by fusing one or more additional targeting moieties to the N-terminus of one or both of the single-chain proteins and / or or C-terminus), so far neither shown nor implied in the prior art

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