Method for preparing guanosine triphosphate by using immobilized enzyme method

Abstract

The invention relates to the technical field of biology and in particular to a method for preparing guanosine triphosphate by using an immobilized enzyme method. The method specifically comprises thefollowing steps: (1) preparing a GTP producing enzyme; (2) immobilizing the GTP producing enzyme; and (3) separating a product. In the method, two GTP producing enzymes including PPK and GMPK are adopted, and GTP can be synthesized by two-step enzymatic reactions; compared with a traditional tedious process for producing GTP by using a beer yeast, the method has the advantages that the reaction process is simpler, a reaction is easier to control, and the quality of a product is more stable; GTP is prepared by adopting an immobilized enzyme catalysis method, and an immobilized enzyme can be continuously and repeatedly used for many times, so that the production cost is greatly reduced; meanwhile, impurities such as pigments and other types of nucleotides introduced by using the yeast are avoided, so that purification is easier to realize; and the method is suitable for producing GTP on a large scale.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for preparing guanosine triphosphate by an immobilized enzyme method. Background technique [0002] Utilizing the glycolysis pathway of yeast and using guanylic acid as a substrate to phosphorylate GTP at the matrix level is the most commonly used method, and it is also the method commonly used in the industrial production of guanosine triphosphate (GTP). However, the reaction process of synthesizing GTP catalyzed by yeast cell enzyme system is complicated, and there are many enzyme systems involved in the catalytic reaction, the reaction process is not easy to control, and the quality of products varies greatly among batches. At the same time, the quality of yeast enzymes often varies greatly due to different suppliers, different batches, and even different seasons. The quality of yeast enzymes is unstable, the enzyme activity decreases rapidly, and the service life is sho...

AI Technical Summary

Problems solved by technology

[0003] In addition, the cost of enzymes is expensive in practical applications. Using free enzymes to produce GTP, the enzyme activity decreases rapidly and cannot be effectively recycled, which will inevitably lead to high production costs and affect the actual application value.