Diaminobenzidine separation and evaluation method based on YCbCr color space

Abstract

The invention discloses a diaminobenzidine separation and evaluation method based on a YCbCr color space. The method comprises the following implementation steps: S1, acquiring a picture; S2, converting to a color space; S3, generating a Crb or Cbr chromaticity difference picture; S4, setting an offset value; S5, extracting symbol information; S6, performing morphological processing on the information in the step S5; S7, generating a final image; the adopted YCbCr color space most conforms to the color composition nature of diaminobenzidine and hematoxylon dyeing, and a positive dyeing area can be separated more accurately; the final image generated by the method can quantitatively describe the dyeing degree; by using the method, higher separation accuracy can be obtained compared with thetraditional method.

Description

technical field [0001] The invention relates to the technical field of computer-aided immunohistochemical staining, in particular to a method for separating and evaluating diaminobenzidine based on YCbCr color space. Background technique [0002] Immunohistochemistry is a very powerful method for detecting target antigens, and the digital image of immunohistochemistry is a three-dimensional data composed of three color channels of red, green and blue (RGB). Computer-aided diagnosis tools need to use mathematical algorithms to segment positively stained pixels from the three-dimensional image to the greatest extent, and mark the degree of staining, and stain the target antigen tissue so that it can be seen under an optical microscope. The reliability and versatility of immunohistochemistry make it widely used in clinical diagnosis and basic research. Diaminobenzidine is one of the most commonly used stains in immunohistochemistry. It can give target tissues a brown color The...

AI Technical Summary

Problems solved by technology

The reliability and versatility of immunohistochemistry make it widely used in clinical diagnosis and basic research. Diaminobenzidine is one of the most commonly used stains in immunohistochemistry. It can give target tissues a brown color The staining (positive staining) to distinguish it from the background and the blue color (negative staining) caused by hematoxylin-diaminobenzidine (H-DAB) stained immunohistochemical staining pathological images, quantitative analysis The distribution and staining intensity of positive staining (DAB) can be used for biomarker localization or immune response scoring, which is of great significance in clinical medicine. However, the methods currently used are all based on manual semi-quantitative assessment of positive staining area and intensity Such as the method of color deconvolution, the method of blue normalization, the method of mathematically transforming the original image matrix to separate positive staining, etc., these methods either have very poor segmentation effects on heavy positive staining, or the adjustment on different databases It is cumbersome, or the generated pictures cannot give a quantitative description of the staining concentration, and it is time-consuming and subject to subjective errors. Therefore, it is particularly important to use computer algorithms to accurately separate and evaluate staining

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