Method for extracting rhizobium japonicum exopolysaccharides

A soybean rhizobia and extraction method technology, applied in the field of soybean rhizobia exopolysaccharide extraction, can solve problems such as unsatisfactory protein removal effect, and achieve the effect of enhancing protein removal rate and improving efficiency

Inactive Publication Date: 2020-01-17
INST OF MICROBIOLOGY HEILONGJIANG ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The present invention aims to solve the technical problem of unsatisfactory protein removal effect in rhizobium exopolysaccharide; and provides a method for extracting soybean rhizobium exopolysaccharide

Method used

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  • Method for extracting rhizobium japonicum exopolysaccharides
  • Method for extracting rhizobium japonicum exopolysaccharides
  • Method for extracting rhizobium japonicum exopolysaccharides

Examples

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Embodiment 1

[0025] Embodiment 1: in the present embodiment, the soybean rhizobium exopolysaccharide extraction method is carried out according to the following steps:

[0026] Step 1. Inoculate the soybean rhizobia HH103 stored at -80°C on a TY plate, put it into a 28°C incubator and incubate it upside down for 3 days, pick a ring of plate seeds and inoculate it into a 10mL test tube of TY liquid medium, 140rpm Shake culture at 28°C for 2 days, and inoculate 5mL of seed liquid into a 500mL Erlenmeyer flask with a 1% inoculum amount, and inoculate it in a 500mL Erlenmeyer flask with a liquid volume of 100mL, and shake at 28°C for 2 days at 140rpm to obtain a soybean rhizobium fermentation broth;

[0027] Centrifuge the soybean rhizobium fermentation broth at a rate of 8000rpm for 5min, take the supernatant, add trichloroacetic acid with a mass concentration of 80% to a final concentration of 4%, let it stand at 4°C for 16h, and then centrifuge at a rate of 8000rpm for 5min , take the super...

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Abstract

The invention discloses a method for extracting rhizobium japonicum exopolysaccharides, belongs to the technical field of rhizobium japonicum exopolysaccharides extraction, and aims to solve the technical problem that the effect of removing protein from rhizobium exopolysaccharides is not ideal. According to the method, serine protease and a Sevage method are adopted for deproteinization treatmentto treat crude exopolysaccharides of rhizobium HH103, the protein removal rate is obviously increased along with increase of the deproteinization frequency, and a linear trend is formed.

Description

technical field [0001] The invention belongs to the technical field of exopolysaccharide extraction of rhizobia, and specifically relates to a method for extracting exopolysaccharide of soybean rhizobia. Background technique [0002] Polysaccharides are a kind of macromolecular polymer with complex structure formed by the dehydration of multiple identical or different monosaccharides to form glycosidic bonds, which are connected linearly or branched by glycosidic bonds. Plants, animals, microorganisms (including bacteria, fungi) and algae and other living organisms. Compared with the cell wall polysaccharides extracted from fungi or plants, the exopolysaccharides secreted by bacteria into the surrounding environment are mainly homogeneous polysaccharides composed of regular repeating units. The molecular weight is generally larger, and its repeating units are usually more than 1000. Due to the unique physicochemical and biological properties of microbial polysaccharides, mo...

Claims

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Application Information

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IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 甄涛于德水戴肖东赵晓宇周舒扬
Owner INST OF MICROBIOLOGY HEILONGJIANG ACADEMY OF SCI
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