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Micro-fluidic chip mass spectrum system used for single cell analysis and application method thereof

A technology of microfluidic chip and mass spectrometry system, which is applied in the field of microfluidic chip mass spectrometry system, can solve the problems of inaccessibility and limitation of standard products, and achieve the effects of eliminating organic phase interference, increasing efficiency and promoting stable progress.

Active Publication Date: 2020-01-14
NORTHEASTERN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its standard products are not easy to obtain, and special single-cell sample loading equipment is required, which limits rapid and high-throughput single-cell detection.

Method used

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  • Micro-fluidic chip mass spectrum system used for single cell analysis and application method thereof
  • Micro-fluidic chip mass spectrum system used for single cell analysis and application method thereof

Examples

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Effect test

Embodiment 1

[0041] A method for using a microfluidic chip mass spectrometry system for single cell analysis to detect single cells, using a microfluidic chip mass spectrometry system for single cell analysis, comprising the following steps:

[0042] Step 1, prepare sample injection: provide Cd-containing sample by micro-syringe pump a12 2+ cell suspension at a flow rate of 200 μL / min and a cell density of 10 6 cells / mL, connected to the inlet Ⅰ4 of the microfluidic control chip through the capillary a2, will contain Cd 2+ The cell suspension is passed into the micron-scale single cell separation channel 6;

[0043] Intracellular Cd 2+ The preparation method of the cell suspension is:

[0044] (1) In the cell culture well plate, culture cells in the first medium to the logarithmic growth phase, remove the first medium and wash;

[0045] (2) In the cell culture well plate, replace it with a certain amount of Cd 2+ The fresh medium of the solution is cultivated for a certain period of t...

Embodiment 2

[0055] A method for using a microfluidic chip mass spectrometry system for single cell analysis to detect single cells, using a microfluidic chip mass spectrometry system for single cell analysis, comprising the following steps:

[0056] Step 1, prepare for sample injection: the cell suspension of the cells incubated in the gold nanoparticle-containing medium is provided by the microsyringe pump a12, the flow rate is 200 μL / min, and the cell density is 10 6 cells / mL, connected to the inlet Ⅰ4 of the microfluidic control chip through the capillary a2, will contain Cd 2+ The cell suspension is passed into the micron-scale single cell separation channel 6;

[0057] Wherein the preparation method of the cell suspension containing gold nanoparticles in the cells is:

[0058] (1) In the cell culture well plate, culture cells in the first medium to the logarithmic growth phase, remove the first medium and wash;

[0059] (2) In the cell culture well plate, replace it with a fresh me...

Embodiment 3

[0069] A method for using a microfluidic chip mass spectrometry system for single cell analysis to detect single cells, using a microfluidic chip mass spectrometry system for single cell analysis, comprising the following steps:

[0070] Step 1, prepare sample injection: provide Cd-containing sample by micro-syringe pump a12 2+ and Cu 2+ cell suspension at a flow rate of 200 μL / min and a cell density of 10 6 cells / mL;

[0071] Intracellular Cd 2+ and Cu 2+ The preparation method of the cell suspension is as follows: ①In the cell culture well plate, culture the cells in the first medium to the logarithmic growth phase, remove the first medium and wash; ②In the cell culture well plate, replace it with Must be Cd 2+ and Cu 2+ The fresh medium of the solution is cultivated for a certain period of time to incubate the cells, in which Cd 2+ Concentration of 100 0μg / L, Cu 2+ The concentration of 100μg / L; ③The incubated cells were digested from the cell culture well plate, and...

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Abstract

The invention discloses a micro-fluidic chip mass spectrum system used for single cell analysis and an application method thereof. The system comprises a capillary tube a, a capillary tube b and a capillary tube c. The micro-fluidic chip is provided with two inlets and an outlet, one ends of the capillary tube a and the capillary tube b are connected with outlet ends of a micro-injection pump a and a micro-injection pump b, and the other ends are connected with an inlet I and an inlet II of the micro-fluidic chip. One end of the capillary tube c is connected with the outlet of the micro-fluidic chip, and the other end is connected with an inductively coupled plasma mass spectrometer through a commercial atomization system. An application method of the micro-fluidic chip mass spectrum system used for single cell analysis for single cell detection comprises the following steps of step 1, preparing for sample introduction; step2, orderly arranging cells; step3, forming a single cell bundle; step4, atomizing a single-cell liquid flow; step 5, carrying out quantitative analysis of single cells; and step 6, carying out repeated determination. The system and the method are not limited byspecific fluid conditions, and stable and efficient single-cell arrangement is formed at a wide flow rate range.

Description

technical field [0001] The invention belongs to the field of mass spectrometry detection equipment and analysis technology, and in particular relates to a microfluidic chip mass spectrometry system for single cell analysis and a use method thereof. Background technique [0002] In recent years, Inductively Coupled Plasma Mass Spectrometry (ICP-MS), as a powerful trace element analysis technology, has the advantages of low detection limit, less interference, high sensitivity, and wide analysis range. The combination of other technologies has been widely used in biomedicine, food hygiene, chemical analysis and other fields to obtain reliable and accurate qualitative and quantitative elemental analysis results. Known as one of the "Top 10 World-Changing Inventions", the microfluidic chip is developing rapidly as an emerging technology. It has the advantages of less sample consumption, high functional integration, and precise scale, and plays a huge role in the field of cell res...

Claims

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Application Information

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IPC IPC(8): G01N27/62B01L3/00G01N27/626
CPCG01N27/62B01L3/5027
Inventor 陈明丽王建华张璇魏星
Owner NORTHEASTERN UNIV
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