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A kind of α-amylase inhibitor and its preparation process and application

An amylase inhibitor and preparation technology, applied in the field of α-amylase inhibitors, to achieve the effects of high purity, mild reaction conditions, and good heat resistance

Active Publication Date: 2021-08-24
完美(广东)日用品有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are few reports on α-amylase inhibitors in seabuckthorn

Method used

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  • A kind of α-amylase inhibitor and its preparation process and application
  • A kind of α-amylase inhibitor and its preparation process and application
  • A kind of α-amylase inhibitor and its preparation process and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] The α-amylase inhibitor provided in this example is a protein isolated from seabuckthorn seed meal, and the preparation process includes:

[0054] The seabuckthorn seed meal was pulverized into fine particles with a pulverizer and sieved to obtain seabuckthorn raw material powder with a particle size of 80 mesh, which was stored in a -20°C refrigerator for later use.

[0055]Add 25 times its weight of 50% ethanol solution to 10 g of raw material powder, and stir at 300 rpm / min for 2 hours at 50° C. to obtain a mixed solution. The resulting mixed solution was filtered with gauze to obtain a filtrate and a first precipitate. The obtained filtrate was subjected to sedimentation and centrifugation, and was centrifuged at 6000 rpm / min for 15 minutes to obtain the second precipitate. The first precipitate obtained and the second precipitate obtained were combined. The resulting combined precipitate was repeated twice. The finally obtained precipitates were combined and dri...

Embodiment 2

[0064] The α-amylase inhibitor provided in this example is a protein isolated from seabuckthorn seed meal, and the preparation process includes,

[0065] The seabuckthorn seed meal was pulverized into fine particles with a pulverizer and sieved to obtain seabuckthorn raw material powder with a particle size of 60 mesh, which was stored in a -20°C refrigerator for later use.

[0066] Add 30 times its weight of 65% ethanol solution to 10 g of seabuckthorn raw material powder, and stir at 500 rpm / min for 0.5 h at 65° C. to obtain a mixed solution. The resulting mixed solution was filtered with gauze to obtain a filtrate and a first precipitate. The obtained filtrate was subjected to sedimentation and centrifugation treatment, and was centrifuged at 5000 rpm / min for 20 minutes to obtain the second precipitate. The resulting combined precipitate was repeated twice. The finally obtained precipitates were combined and dried at 45° C. for 2 hours to obtain seabuckthorn seed meal pro...

Embodiment 3

[0071] The α-amylase inhibitor provided in this example is a protein isolated from seabuckthorn seed meal, and the preparation process includes,

[0072] The seabuckthorn seed meal was crushed into fine particles with a pulverizer and sieved to obtain seabuckthorn raw material powder with a particle size of 100 mesh, which was stored in a -20°C refrigerator for later use.

[0073] Add 20 times its weight of 75% ethanol solution to 10 g of raw material granules (raw material powder), stir at 45° C. and 200 rpm / min for 3 h to obtain a mixed solution. The resulting mixed solution was filtered with gauze to obtain a filtrate and a first precipitate. The obtained filtrate was subjected to sedimentation and centrifugation treatment, and was centrifuged at 8000 rpm / min for 10 minutes to obtain the second precipitate. The resulting combined precipitate was repeated twice. The finally obtained precipitates were combined and dried at 60° C. for 0.5 h to obtain seabuckthorn seed meal p...

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Abstract

The invention discloses an α-amylase inhibitor in the technical field of preparation of natural active substances, its preparation process and application. The preparation process of the α-amylase inhibitor includes preparing seabuckthorn protein; adding deionized water to the seabuckthorn protein, adjusting the pH value of the solution to 6.0-8.0, stirring at 30-55°C for 1-2 hours, and centrifuging for the first time to obtain The first supernatant and precipitation; freeze-drying the first supernatant to obtain α-amylase inhibitor I. Adding sodium chloride solution to the precipitate, adjusting the pH of the solution to 7.0-9.0, stirring at 35-50°C for 0.5-2.5 hours, centrifuging for the second time to obtain the second supernatant; freeze-drying the second supernatant , to obtain α-amylase inhibitor II. The preparation process of the invention has mild reaction conditions, simple operation and low equipment investment, and is suitable for industrialized production. The prepared α-amylase inhibitor has the advantages of high purity, strong inhibitory activity, good heat resistance, no food additive residue, non-toxic and safe products, and the like.

Description

technical field [0001] The invention relates to the technical field of preparation of natural active substances, in particular to an α-amylase inhibitor and its preparation process and application. Background technique [0002] α-amylase inhibitor (α-AI for short) is a pure natural biologically active substance, which exists in the endosperm of plant seeds and metabolites of microorganisms, and has a wide range of sources. At present, α-amylase inhibitors are mainly extracted and isolated from plant seeds such as wheat, rice, sorghum, peanuts, and beans. The sources of α-amylase inhibitors are different, and the methods of separation and purification are also different. [0003] "Separation, Purification, Structure and Function Research of Novel α-Amylase Inhibitors in Wild Amaranthus Seeds", Zhang Lin et al. Soaked wild Amaranth seeds with sodium chloride, ground and centrifuged to get the supernatant, and tested wild Amaranth The plant seeds were repeatedly extracted 3 t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C07K1/30A61K38/16A61P3/04A61P3/10A23L33/185
CPCA23V2002/00A61K38/168A23L33/185A61P3/04A61P3/10C07K14/415A23V2200/328A23V2200/332A23V2250/548
Inventor 刘洪霞李家炜崔春孙允武夏涛李晓敏欧阳道福古润金
Owner 完美(广东)日用品有限公司
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