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Alpha-amylase inhibitor and preparation technology and application thereof

An amylase inhibitor and the technology of its preparation process are applied in the field of α-amylase inhibitors to achieve the effects of lowering blood sugar, mild reaction conditions and simple operation

Active Publication Date: 2020-01-14
完美(广东)日用品有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are few reports on α-amylase inhibitors in seabuckthorn

Method used

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  • Alpha-amylase inhibitor and preparation technology and application thereof
  • Alpha-amylase inhibitor and preparation technology and application thereof
  • Alpha-amylase inhibitor and preparation technology and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] The α-amylase inhibitor provided in this example is a protein isolated from seabuckthorn seed meal, and the preparation process includes:

[0054] The seabuckthorn seed meal was pulverized into fine particles with a pulverizer and sieved to obtain seabuckthorn raw material powder with a particle size of 80 mesh, which was stored in a -20°C refrigerator for later use.

[0055]A 50% ethanol solution of 25 times its weight was added to 10 g of the raw material powder, and the mixture was stirred at 300 rpm / min for 2 hours at a temperature of 50° C. to obtain a mixed solution. The resulting mixed solution was filtered with gauze to obtain a filtrate and a first precipitate. The obtained filtrate was subjected to sedimentation centrifugation, and centrifuged at 6000 rpm / min for 15 min to obtain a second precipitate. The resulting first precipitate and the resulting second precipitate are combined. The resulting combined precipitate was repeated the above operation twice. ...

Embodiment 2

[0064] The α-amylase inhibitor provided in this embodiment is a protein isolated from sea buckthorn seed meal, and the preparation process includes,

[0065] The sea buckthorn seed meal was pulverized into fine particles with a pulverizer and sieved to obtain sea buckthorn raw material powder with a particle size of 60 meshes, which was stored in a -20°C refrigerator for later use.

[0066] A 65% ethanol solution of 30 times its weight was added to 10 g of seabuckthorn raw material powder, and the mixture was stirred for 0.5 h at 500 rpm / min at a temperature of 65° C. to obtain a mixed solution. The resulting mixed solution was filtered with gauze to obtain a filtrate and a first precipitate. The obtained filtrate was subjected to sedimentation centrifugation, and centrifuged at 5000 rpm / min for 20 min to obtain the second precipitate. The resulting combined precipitate was repeated the above operation twice. The final obtained precipitates were combined and dried at 45° C. ...

Embodiment 3

[0071] The α-amylase inhibitor provided in this embodiment is a protein isolated from sea buckthorn seed meal, and the preparation process includes,

[0072] The sea buckthorn seed meal was pulverized into fine particles with a pulverizer and sieved to obtain sea buckthorn raw material powder with a particle size of 100 meshes, which was stored in a -20°C refrigerator for later use.

[0073] 20 times its weight of 75% ethanol solution was added to 10 g of raw material particles (raw material powder), and stirred at 45° C. for 3 hours at 200 rpm / min to obtain a mixed solution. The resulting mixed solution was filtered with gauze to obtain a filtrate and a first precipitate. The obtained filtrate was subjected to sedimentation centrifugation, and centrifuged at 8000 rpm / min for 10 min to obtain the second precipitate. The resulting combined precipitate was repeated the above operation twice. The final obtained precipitates were combined and dried at a temperature of 60° C. for...

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Abstract

The present invention discloses an alpha-amylase inhibitor in the technical field of natural active substance preparation, and a preparation technology and an application of the alpha-amylase inhibitor. The preparation technology of the alpha-amylase inhibitor comprises the following steps: preparing sea buckthorn protein, adding deionized water to the sea buckthorn protein, adjusting a solution pH to 6.0-8.0, conducting stirring at 30-55 DEG C for 1-2 h, and conducting first centrifugal separation to obtain a first supernatant and a precipitate; freeze-drying the first supernatant to obtain an alpha-amylase inhibitor I, adding a sodium chloride solution to the precipitate, adjusting the solution pH to 7.0-9.0, conducting stirring at 35-50 DEG C for 0.5-2.5 h, and conducting second centrifugal separation to obtain a second supernatant; and freeze-drying the second supernatant to obtain an alpha-amylase inhibitor II. The preparation technology is mild in reaction conditions, simple in operation, low in equipment investment, and suitable for industrial production. The prepared alpha-amylase inhibitor has advantages of being high in purity, strong in inhibitory activity, good in heatresistance, free of residue of food additives, non-toxic, safe, etc.

Description

technical field [0001] The invention relates to the technical field of preparation of natural active substances, in particular to an α-amylase inhibitor and its preparation process and application. Background technique [0002] α-amylase inhibitor (α-AI for short) is a pure natural biologically active substance, which exists in the endosperm of plant seeds and metabolites of microorganisms, and has a wide range of sources. At present, α-amylase inhibitors are mainly extracted and isolated from plant seeds such as wheat, rice, sorghum, peanuts, and beans. The sources of α-amylase inhibitors are different, and the methods of separation and purification are also different. [0003] "Separation, Purification, Structure and Function Research of Novel α-Amylase Inhibitors in Wild Amaranthus Seeds", Zhang Lin et al. Soaked wild Amaranth seeds with sodium chloride, ground and centrifuged to get the supernatant, and tested wild Amaranth The plant seeds were repeatedly extracted 3 t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C07K1/30A61K38/16A61P3/04A61P3/10A23L33/185
CPCA23V2002/00A61K38/168A23L33/185A61P3/04A61P3/10C07K14/415A23V2200/328A23V2200/332A23V2250/548
Inventor 刘洪霞李家炜崔春孙允武夏涛李晓敏欧阳道福古润金
Owner 完美(广东)日用品有限公司
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