Composite mycorrhiza biological fertilizer, and preparation method and application thereof

Abstract

The invention belongs to the technical field of microbial fertilizers and preparations, and discloses a composite mycorrhiza biological fertilizer, and a preparation method and an application thereof.The preparation method comprises the following steps: activating a preserved strain with a solid activation medium, picking single colonies, culturing the single colonies on a same medium plate according to pairwise crossed three-zone lines of member strains in groups at 30 DEG C for 3 days, and repeating the culture for 3 times; inoculating antagonistic bacteria-free strains and mycorrhizal fungi into respective liquid culture media; preparing 100 ml of a solution from sodium alginate with the concentration of 3% and polyvinyl alcohol with the concentration of 3% at 35 DEG C; adding 0.5% biochar, preparing 100 ml of a solution at 35 DEG C, sterilizing the solution at 121 DEG C for 20 min, cooling the sterilized solution to 35 DEG C, taking 2 g of composite functional bacteria and 2 g ofmycorrhizal fungi thalli, adding the taken substances into an embedding medium solution, and performing uniform mixing in a constant-temperature magnetic stirrer. The functional bacterial system and mycorrhizal fungus mixing embedding immobilization research and the embedding immobilized particle promote Chinese pine seedlings.

Description

technical field [0001] The invention belongs to the technical field of microbial fertilizers and preparations, and in particular relates to a compound mycorrhizal biological fertilizer and its preparation method and application. Background technique [0002] At present, the closest existing technology: Academician Chen Huagui, the founder of soil microbiology in China, defines microbial fertilizers as a type of "specific products containing live microorganisms, and the fertilizer effect obtained by plants mainly depends on the life activities of living microorganisms in the products. Microorganisms Fertilizers are used in agricultural and forestry production. After being applied to the soil, there are many types of growth-promoting bacteria or bio-control bacteria that stably colonize the root soil of plants to form a specific microbial preparation of dominant flora. It has specific fertilizers such as phosphorus, potassium, nitrogen fixation and pest control. Effect, and in...

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Problems solved by technology

[0011] (2) Some enterprises in the field of micro-fertilizers are slow to update equipment, have primitive production technology, and poor product quality

In addition, the application effect of micro-fertilizers is unstable, which is affected by many factors such as application methods and environmental conditions, which hinders the further promotion and application of the micro-fertilizer industry

[0013] (3) PGPR cannot be stably colonized in micro-fertilizers

At present, most enterprises are still using Bacillus-based nitrogen-fixing, phosphorus- and potassium-solubilizing bacteria, but fungal functional bacteria such as VA and ECM are rarely used.

[0015] (4) Research on the mechanism of action of micro-fertilizer products is not deep enough

[0016] Studies have shown that the mechanism of PGPR promoting plant growth in micro-fertilizers is divided into direct mechanism and indirect mechanism. The difference between the two mechanisms is mainly based on the location of the mechanism. The PGPR effect occurs outside the plant as an indirect mechanism, and the PGPR effect occurs inside the plant as a direct mechanism. Mechanism, the direct mechanism of PGPR to promote plant growth is still in the study of biological nitrogen fixation, phosphorus and potassium, plant hormone production indole-3-acetic acid (IAA), cytokinin (CK), gibberellins (GAs), etc. The indirect mechanism research is limited to siderophore production, lyase production, antibiotic production, induction of plant systemic resistance and ACCD, etc., while the interaction mechanism between bacteria and bacteria and the interaction between bacteria and plants in the soil micro-ecological environment Mechanism research is not detailed enough

[0017] (5) The national micro-fertilizer management is not enough

The adsorption method is simple to operate, the reaction conditions are mild, and it is non-toxic and harmless to the cells. The disadvantage is that the combination is not firm, and the cells are easy to fall off. The direct contact between the cells and the soil environment is greatly affected by environmental changes.

[0052] In addition to the adsorption method, there are two immobilization methods, the binding method and the cross-linking method. These two methods react violently in immobilization, the materials used are highly toxic, and the cell survival rate is low, so they are not suitable for the immobilization of living cells.

[0053] In summary, the existing problems in the prior art are: the existing immobilization methods have severe reactions, the materials used are highly toxic, and the cell survival rate is low, so they are not suitable for the immobilization of living cells.

At present, most of the microbial fertilizers on the market are liquid and powder, but the combination of microorganisms and carriers is not strong, and the cells are easy to fall off. They are exposed in the soil environment and are greatly affected by environmental changes, while colloidal agents can embed more cells. , a higher cell concentration can be obtained in a short time, the cells can be evenly distributed in the gel grid, and the microenvironment of the immobilized cells after embedding is suitable for cell growth and rapid proliferation

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