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Medium and method for increasing survival rate and achieving high-quality cultivation of dendranthema zawadskii tissue culture seedlings

A technology of wild chrysanthemum group and survival rate, applied in the directions of planting substrate, cultivation, culture medium, etc., can solve the problems of high-efficiency transplanting and planting of tissue culture seedlings of wild chrysanthemum, which can promote large-scale cultivation and market application, and improve survival. rate, the effect of improving the survival rate

Inactive Publication Date: 2020-01-10
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no detailed reports on the high-efficiency transplantation of plantlets of Violet chrysanthemum

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A substrate for improving the survival rate and high-quality cultivation of purple wild chrysanthemum tissue culture seedlings, the composition and mass ratio of the substrate are: sawdust: peat: pearl = 1~3:5~6:0.5~1.2. In this embodiment, the composition and mass ratio of the matrix is: sawdust:peat:pearl=2:7:1. The matrix also contains pig manure, and the content of the pig manure is 1 kg of pig manure per cubic meter of the matrix. The matrix also contains a disinfectant, and the disinfection components of the disinfectant are: 600 times of 50% carbendazim wettable powder, 1700-2000 times of 97% hymexazol, 400-600 times of 80% mancozeb wettable powder , 40% azoxystrobin 800-1000 times.

[0033] The pearl is granular, and the granular pearl contains natural pearl powder, chemically synthesized pearl powder, and auxiliary materials, and the auxiliary materials are a mixture of sepiolite and kaolin. The mixture of sepiolite and kaolin is 5:2 by mass. Granular pearl ...

Embodiment 2

[0035] In conjunction with the substrate in Example 1, a method for improving the survival rate and high-quality cultivation of Violet chrysanthemum tissue culture seedlings, the method uses the substrate as described above, and in the present embodiment, the method includes the following steps:

[0036] (1) Hardening of tissue culture seedlings: During April-May every year, root and cultivate tissue culture seedlings with a height of 7-10cm in the tissue culture room, and harden the seedlings with natural light in the field for 7 days in the whole bottle, and then open the tissue culture bottle cap Seedling hardening in natural environment for 2 days.

[0037] (2) Cleaning of tissue-cultured seedlings: Take the tissue-cultured seedlings out of the bottle, wash them with clean water, remove the root medium and old yellow leaves, drain the water, and air-dry naturally.

[0038] (3) Cultivation substrate treatment: Spray the substrate with disinfectant for substrate disinfection...

Embodiment 3

[0045] In conjunction with Example 2, in this example: in Chun'an, Zhejiang, the domesticated varieties of Violet chrysanthemum were collected for tissue culture in Hangzhou, and 200 bottles of rooted tissue culture seedlings with a height of 7-10cm were selected in mid-April, 2017, with an average of 10 bottles per bottle. Trees were transplanted in Hangzhou greenhouse.

[0046] Specific operation steps: Wash the tissue-cultured seedlings with clean water, drain the water, and air-dry naturally. Use medicaments (50% carbendazim WP 600 times, 97% hymexazol 1700-2000 times, 80% mancozeb WP 400-600 times, 40% azoxystrobin 800-1000 times) disinfection configuration good substrate. The dried tissue-cultured seedlings were inserted into the treated peat substrate for cutting culture. After 1-2 months of growth, cut off the 7-10cm shoots and dip them in rooting powder. Mix the rooting coconut peat matrix with disinfectants (50% carbendazim WP 600 times, 80% mancozeb WP 400-600 ti...

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Abstract

The invention discloses a medium and a method for increasing the survival rate and achieving high-quality cultivation of dendranthema zawadskii tissue culture seedlings, and belongs to the technical field of plant cultivation. Components of the medium, including wood chips, peat and pearls, are in a ratio of 2:7:1. The method includes the steps of domestication of the tissue culture seedlings, cleaning of the tissue culture seedlings, culture medium treatment, primary culture treatment, scion harvesting treatment, rooting medium treatment, seedling rooting treatment, and seedling planting treatment. The medium and the method for increasing the survival rate and achieving high-quality cultivation of the dendranthema zawadskii tissue culture seedlings have the advantages that the survival rate of the dendranthema zawadskii tissue culture seedlings is increased, and high-quality cultivation of dendranthema zawadskii is achieved; through the perfect efficient culture medium and the perfectcultivation method for the dendranthema zawadskii, large-scale cultivation and market application of the dendranthema zawadskii are promoted.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation and specifically relates to a substrate and a method for improving the survival rate and high-quality cultivation of purple wild chrysanthemum tissue culture seedlings. Background technique [0002] Purple wild chrysanthemum is a perennial herb of the genus Chrysanthemum in the family Compositae. It is used as one of the edible flowers of medicinal chrysanthemum for its functions of clearing away heat and detoxifying, drying pus and reducing swelling. As my country's demand for medicinal chrysanthemums increases year by year, there are greater requirements for the cultivation and planting of purple wild chrysanthemums. However, how to maintain the quality of seedlings and adapt to the ever-increasing market has become the main way to solve these problems through the efficient organization and rapid propagation of purple wild chrysanthemum. But the transplanting survival rate of the purp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G2/10A01G7/06A01G24/20A01G24/23A01G24/28A01H4/00A01N47/18A01N43/80A01N47/14A01N43/54A01P1/00
CPCA01G7/06A01H4/001A01H4/008A01N43/54A01N43/80A01N47/14A01N47/18A01G2/10A01G22/00A01G24/20A01G24/23A01G24/28
Inventor 吴超郭方其彭娟林森红黎侠丁晓瑜
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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