Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of phosphorylated muramyl dipeptide for preparing medicine capable of constructing fulminant liver injury animal model

A technology of phosphorylated muramic acid dipeptide and animal models, which can be applied to dipeptide components, pharmaceutical formulations, organic active ingredients, etc., and can solve problems such as inability to fully reflect immune fulminant liver damage

Active Publication Date: 2020-01-07
THE SECOND AFFILIATED HOSPITAL OF CHONGQING MEDICAL UNIV
View PDF13 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing models are still unable to fully reflect the mechanism of immune fulminant liver injury. Therefore, this field is still studying the method of constructing a new animal model of immune fulminant liver injury

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of phosphorylated muramyl dipeptide for preparing medicine capable of constructing fulminant liver injury animal model
  • Application of phosphorylated muramyl dipeptide for preparing medicine capable of constructing fulminant liver injury animal model
  • Application of phosphorylated muramyl dipeptide for preparing medicine capable of constructing fulminant liver injury animal model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] 1. Animal model of fulminant liver injury constructed by L18-MDP combined with D-GalN:

[0036] Reagents used: the ligand of the intracellular pattern recognition receptor NOD2 is MDP, the degradation product of peptidoglycan on the bacterial cell wall, and its artificially synthesized phosphorylated derivative is L18-MDP, which is purchased from Invivogen Company, product number tlrl-lmdp. In addition, the D-GalN used was purchased from sigma company, item number G0500.

[0037] (1) Female C57BL / 6 mice, 4-6 weeks old, weighing about 14-15g, divided into 4 groups, including blank control group, L18-MDP treatment group, D-GalN treatment group, L18-MDP combined with D-GalN treatment group . Among them, L18-MDP was injected into the tail vein (10ug / mouse), and D-GalN was injected intraperitoneally (20mg / mouse). Fresh eye blood and mouse liver, lung, kidney, small intestine and large intestine samples were collected 16 hours after injection for subsequent analysis.

[003...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the technical field of animal model construction, in particular to application of phosphorylated muramyl dipeptide for preparing a medicine capable of constructing fulminant liver injury animal model. D-galactosamine and the phosphorylated muramyl dipeptide are adopted for jointly constructing the fulminant liver injury animal model, an animal serum ALT (Alanine transaminase) level can be induced to sharply rise under a situation that a proper dosage is given, and hepatic cells can be protected from massive necrosis. Since an injury phenomenon happens in short time after injection, a determination result shows that the fulminant liver injury animal model is successfully constructed.

Description

technical field [0001] The invention relates to the technical field of animal model construction, in particular to the application of phosphorylated muramic acid dipeptide in the preparation of drugs for constructing animal models of fulminant liver injury. Background technique [0002] Clinically, fulminant liver injury is a life-threatening emergency. Studies have shown that liver tissue and cell damage mediated by the body's immune response is involved in the occurrence and development of fulminant liver injury, but the specific mechanism remains to be further studied. Liver injury caused by different etiologies has different relatively specific immune response characteristics. Establishing an ideal animal model of immune fulminant liver injury is one of the necessary conditions for studying the mechanisms of various immune liver injuries. [0003] NOD2 is a pattern recognition receptor that mainly exists in cells. In recent years, attention to NOD2 has been increasing. ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K38/05A61K31/7008A01K67/027
CPCA61K38/05A61K31/7008A01K67/027A01K2267/03
Inventor 殷文伟蔡英任红
Owner THE SECOND AFFILIATED HOSPITAL OF CHONGQING MEDICAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com