Micro-fluidic chip, protein detecting method, protein detecting device and protein detecting system

A microfluidic chip and protein detection technology, applied in the field of protein detection, can solve the problems of poor consistency and repeatability of trace protein detection, unstable immune reaction process, inconvenient and effective control, etc. The effect of unstable reaction and short reaction time

Pending Publication Date: 2020-01-03
SHENZHEN INST OF ADVANCED TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Embodiments of the present invention provide a microfluidic chip, a bipolar electrode chip, a bipolar electrode manufacturing process, a protein detection system, a protein detection device, and a protein detection method to solve In the prior art, due to the instability of the immune reaction process, it is inconvenient to carry out effective control, which makes the consistency and repeatability of trace protein detection poor

Method used

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  • Micro-fluidic chip, protein detecting method, protein detecting device and protein detecting system
  • Micro-fluidic chip, protein detecting method, protein detecting device and protein detecting system
  • Micro-fluidic chip, protein detecting method, protein detecting device and protein detecting system

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Embodiment 1

[0059] In order to solve the problem in the prior art that the consistency and repeatability of trace protein detection are poor due to the instability of the immune reaction process and the inconvenient effective control, Embodiment 1 of the present invention provides a protein detection system 10, please refer to figure 1 , the protein detection system 10 includes a microfluidic chip 101, a bipolar electrode chip 102 and a concentration determination component 103, wherein:

[0060] The microfluidic chip 101 includes a plurality of microwells arranged in an array, and the microwells are used to capture the solid-phase carrier in the sample to be tested; the sample to be tested includes: Solid phase carrier, solid phase carrier unbound with test protein and electrochemiluminescent material, electrochemical reaction solution;

[0061] The bipolar electrode chip 102 includes a plurality of electrode units arranged in an array, and when the electrode units are activated in the s...

Embodiment 2

[0078] Embodiment 2 of the present invention provides a microfluidic chip, please refer to image 3 ,include:

[0079] The solid-phase carrier tiled area 101-1, the solid-phase carrier tiled area 101-1 includes a plurality of liquid diversion grooves, and micro-wells located at the bottom of the liquid diversion grooves, the micro-wells in the microfluidic Arranged in an array on the chip.

[0080] The solid phase carrier tiling area is designed as a two-layer structure. The upper layer is a liquid diversion groove structure with a diversion width of 5-50 μm and a diversion depth of 10-30 μm. The liquid in the sample loading area can pass through the gross suction of the diversion structure. Spread the liquid freely in the tiled area, and flow in a uniform direction. The lower layer is a microwell array structure, and each microwell can only accommodate one magnetic bead position. Under the action of the evaporation effect of the liquid, the magnetic beads in the liquid wil...

Embodiment 3

[0086] Embodiment 3 of the present invention provides a bipolar electrode chip 102, please refer to Figure 4 ,include:

[0087] A plurality of electrode units 102-1 arranged in an array, when the electrode units 102-1 are activated in the sample to be tested, electrochemically react with the electrochemiluminescence material and emit light;

[0088] The area of ​​the anode end 102-1-2 of the electrode unit is smaller than the area of ​​the cathode end 102-1-1.

[0089] The area of ​​the anode end of the bipolar electrode is small, while the area of ​​the cathode end is large. Since the total charge of the electrode is neutral, the current density of the anode will be higher and the luminous intensity will be stronger.

[0090] A preferred embodiment, in the technical solution proposed in Example 3 of the present invention, the cross-section of the electrode unit parallel to the electrode plate is triangular, such as Figure 4 As shown, one side of the triangle is the cathode,...

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Abstract

The invention discloses a protein detecting system which aims at improving the consistency and repeatability of trace protein detection. The protein detecting system comprises a micro-fluidic chip, anambipolar electrode chip and a concentration determining assembly, wherein the micro-fluidic chip comprises a plurality of micropits in array distribution; the micropits are used for capturing solid-phase carriers in a tested sample; the tested sample comprises solid-phase carriers combined with tested protein and an electrochemical luminescent material, solid-phase carriers not combined with thetested protein and the electrochemical luminescent material, and an electrochemical reaction solution; the ambipolar electrode chip comprises a plurality of electrode units in array distribution; when being activated in the tested sample, the electrode units take an electrochemical reaction with the electrochemical luminescent material and give out light; and the concentration determining assembly determines the concentration value of the tested protein in the tested sample according to the total quantity of the solid-phase carriers in the micropits and the quantity of the luminescent solid-phase carriers. In addition, the embodiment of the invention also provides the micro-fluidic chip, the ambipolar electrode chip, a protein detecting method and a protein detecting device.

Description

technical field [0001] The invention relates to the field of protein detection, in particular to a microfluidic chip, a bipolar electrode chip, a bipolar electrode manufacturing process, a protein detection system, a protein detection device and a protein detection method. Background technique [0002] With the rapid development of translational medicine and precision medicine, profound changes have taken place in the research on basic biological units such as cells, nucleic acids, and proteins. At present, it has shifted from the traditional research on the ensemble and averaged responses of these units to the single Cellular and single-molecule level studies. Especially in the field of molecular diagnosis, it is necessary to accurately diagnose the patient's health level and treatment effect at the single-molecule level, so as to facilitate early diagnosis, greatly advance the diagnosis and treatment window, and achieve the effect of preventive treatment. Therefore, it i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/416G01N21/76
CPCG01N21/76G01N27/416
Inventor 吴天准蒋伯石彭智婷
Owner SHENZHEN INST OF ADVANCED TECH
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