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Jatropha curcas L MYB class transcription factor JcMYB16 gene and application in improving plant drought resistance

A transcription factor and drought resistance technology, applied in the field of plant biology, to achieve the effect of improving drought stress resistance

Active Publication Date: 2020-01-03
ZHOUKOU NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In conclusion, although many MYB proteins have been cloned and functionally analyzed, genes of the Jatropha MYB family in response to drought stress are still rarely reported

Method used

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  • Jatropha curcas L MYB class transcription factor JcMYB16 gene and application in improving plant drought resistance
  • Jatropha curcas L MYB class transcription factor JcMYB16 gene and application in improving plant drought resistance
  • Jatropha curcas L MYB class transcription factor JcMYB16 gene and application in improving plant drought resistance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A rice transgenic line acquisition and phenotype, drought stress analysis experiment

[0040] 1 Materials and methods

[0041] 1.1 Plant materials and planting methods

[0042] The rice variety used in the test is the japonica rice variety Zhonghua 11, namely Oryza sativa L. cv. Zhonghua 11, which is kept in the Key Laboratory of Plant Genetics and Molecular Breeding of Zhoukou Normal University. Before planting, the newly harvested seeds were first disinfected with 5% sodium hypochlorite for 40 minutes or 1 / 1000 carbendazim for 12 hours, and then 0.1mol / L HNO 3 (1 mL of 63% concentrated HNO 3 Add 100mL of water) to soak the seeds for 16 hours, rinse with tap water, place the sterilized seeds at 28°C for 1 day, and then spread the seeds evenly in a petri dish, in which a layer of filter paper is placed and moistened with water; Put the lid on, then put it into the culture at 32°C, change the water two to three times a day, and transfer it to 30°C for culture after se...

Embodiment 2

[0072] Obtaining a transgenic line of Arabidopsis thaliana and analyzing its phenotype and drought stress

[0073] 1 Materials and methods

[0074] 1.1 Plant materials and planting methods

[0075] The Arabidopsis thaliana used in this experiment is Columbia ecotype, which is preserved in the Key Laboratory of Plant Genetics and Molecular Breeding of Zhoukou Normal University. The disinfection method of Arabidopsis thaliana seeds is as follows: wash once with sterile water, disinfect with 70% alcohol for 5 minutes, wash once with sterile water, disinfect with 1% sodium hypochlorite for 15 minutes, wash with sterile water five times. Arabidopsis seeds were treated with a low temperature of 4°C for 2 days before planting to make them germinate uniformly. The sterilized seeds were germinated on 1 / 2MS (1% sucrose, 8% agar, pH 5.7) medium for 7-10 days, and then transplanted into the planting medium. Seeds that do not need to be sterilized can be sown directly after low temperat...

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Abstract

The invention provides a Jatropha curcas L MYB class transcription factor JcMYB16 gene. A gene nucleotide sequence of the Jatropha curcas L MYB class transcription factor JcMYB16 gene is SEQ ID NO.1,a nucleotide sequence of a gene open reading frame of the Jatropha curcas L MYB class transcription factor JcMYB16 gene is SEQ ID NO.2, overexpression of JcMYB16 gene does not affect plant growth anddevelopment, and drought stress resistancecan be significantly improved. The gene can be used in the cultivation of drought resistance varieties of cereal crops, such as Jatropha curcas L, rice and wheat, and can further be used in the cultivation of drought resistance varieties of crops, such as barley, sorghum, corn, arabidopsis, tomatoes, tobacco, soybeans and potatoes.

Description

technical field [0001] The invention belongs to the field of plant biotechnology, and relates to a Jatropha MYB transcription factor JcMYB16 gene and its application in plants. Background technique [0002] Physiological water shortage in plants caused by drought stress seriously affects plant growth and crop yield. Statistics show that the arable land area in my country is only 121 million hectares at present, while the desertification and salinization land has 260 million hectares, and the abandoned land caused by human factors is 13 million hectares. Therefore, drought stress has become a bottleneck restricting the growth and development of crops in my country. An important way to solve this problem is to vigorously strengthen the mining of genes related to stress tolerance and the development and utilization research in molecular breeding. At present, the research on the model plant Arabidopsis has preliminarily understood the signaling pathways of plants responding to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/84C12N1/21A01H5/00A01H6/20
CPCC07K14/415C12N15/8273
Inventor 唐跃辉王健包欣欣齐静刘坤
Owner ZHOUKOU NORMAL UNIV
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