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Improved method for preparing mouse silicosis model

A mouse and model technology, which is applied in animal husbandry and other fields, can solve the problems that mouse models cannot be successfully prepared, cannot be widely used, and the modeling time is long, so as to ensure repeatability and scientificity, keep the airway unobstructed, The effect of high model success rate

Active Publication Date: 2022-01-28
NORTH CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

SiO2 nebulized daily with the dust system 2 Dust, this method can best simulate the pathogenic process of human dust exposure, which is more in line with the natural progression of silicosis, and has been successfully used in the preparation of rat models; however, this method is limited by special equipment conditions, dust costs and manufacturing costs. Due to the limitations of factors such as long mold time, it cannot be widely used
More importantly, domestic and foreign and the preliminary research of the present invention use micron-sized SiO 2 Dust-infected mice (C57BL / 6 mice were exposed to dust for 6 months, ICR mice were exposed to dust for 2 months) and nanoscale SiO 2 Dust-exposed mice (both C57BL / 6 mice and ICR mice were exposed to dust for 2 months), the results showed that daily nebulized SiO inhalation using the dust-exposed system 2 Dust, SiO in different particle sizes 2 There are very few deposits in the lungs of different strains of mice, and the mouse model cannot be successfully prepared

Method used

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  • Improved method for preparing mouse silicosis model
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  • Improved method for preparing mouse silicosis model

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Improved method for preparing mouse silicosis model

[0041] 1. Experimental animals

[0042] SPF-grade C57BL / 6 mice (provided by the Experimental Animal Center of North China University of Science and Technology), both male and female, aged 8-16 weeks, weighing 20-30 g.

[0043] 2. Preparation before model preparation

[0044] (1) Micron-sized SiO 2 The particles were ground in an agate mortar for 2-3 hours, and then baked in a vacuum oven at 200°C for 2 hours to sterilize and remove endotoxin contamination; it was prepared with a concentration of 50mg / ml, 100mg / ml, 200mg / ml SiO 2 Suspension.

[0045] (2) The mice were fasted for 8 hours before operation.

[0046] 3. Model preparation process

[0047] (1) Anesthesia: the mice were anesthetized by intraperitoneal injection of 1% pentobarbital sodium (the mass fraction of pentobarbital sodium is 50 mg / kg).

[0048] (2) Fixing and shearing: Fix the mouse in the supine position, with the neck raised slightly, and the ...

Embodiment 2

[0056] In the mouse silicosis model prepared by the improved method disclosed in this application, different concentrations of SiO were instilled into the trachea in groups. 2 Suspension, specifically: Group A was injected with 50 mg / mL SiO 2 Suspension, group B was injected with 100mg / mL SiO 2 Suspension, group C was injected with 200mg / mL SiO 2 Suspension, another control group was set up, and the success rate and death rate of the 4 groups of mouse models were observed respectively, as shown in Table 1.

[0057] Table 1 The success rate and mortality rate of the mouse model

[0058]

[0059] Note: 1. The total number of deaths is equal to the number of deaths during the operation plus the number of deaths after the operation; 2.171 / 223 represents the survival number of the model and the total survival number (including the survival number of the control group and the survival number of the model group), explanation: 171=60+57 +55, 223=51+60+57+55; 3. The success rate ...

Embodiment 3

[0065] Compare the number of nodules in the animal model prepared by different methods and the mouse model prepared by the improved method of the present application (nodule is one of the criteria for judging silicosis)

[0066] 1. References: Quan Shangkun et al., Intervention effect of panobinostat on silicosis fibrosis in mice, intranasal instillation of SiO 2 The mouse model was prepared by the suspension method, and the nodules of the lung tissue sections were observed, such as Figure 10 shown;

[0067] 2. References: Sachin K et al. Oropharyngeal administration of silica in Swiss mice: A robust and reproducible model of occupational pulmonary fibrosis, using oropharyngeal inhalation of SiO 2 The mouse model was prepared by the suspension method, and the nodules of the lung tissue sections were observed, such as Figure 11 shown.

[0068] 3. References: Wu Q et al, miR-489 inhibits silica-induced pulmonary fibrosis by targeting MyD88 and Smad3 and is negatively regula...

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Abstract

The invention discloses an improved method for preparing a mouse silicosis model, which comprises the following steps: step 1: pretreating the mouse, separating the anterior neck muscles and exposing the trachea; Gently pull out the mouth, use a 20g blunt needle to pass through the anterior wall of the oropharynx, and insert it into the trachea about 2-3cm under direct vision; Step 3: Quickly inject 50 μL of SiO into the trachea with a syringe 2 After the suspension, remove the syringe, connect the needle to the ventilator, and ventilate for 10-30s; Step 4: When the normal breathing of the mouse resumes, remove the ventilator, and continue to observe the normal breathing, pull out the needle; Step 5 : Suture the skin, and perform post-care and observation. The method is simple and easy to operate, simulates the dust contamination process of humans to a high degree, and has the advantages of low mouse death rate, high model success rate, and strong operability.

Description

technical field [0001] The invention belongs to the technical field of preparation of silicosis animal models, and in particular relates to an improved method for preparing mouse silicosis models. Background technique [0002] Silicosis is due to prolonged inhalation of SiO 2 Dust is a common occupational disease mainly manifested by the formation of silicon nodules and diffuse pulmonary interstitial fibrosis. Understanding the pathogenesis and prevention measures of silicosis depends on the establishment of animal models of the disease. At present, there are many ways to establish small animal silicosis models, such as: tracheal intubation (tracheostomy) instillation of SiO 2 Suspension, intranasal instillation, and oropharyngeal inhalation of SiO 2 Suspension, inhalation of SiO by daily nebulization with dust system 2 dust etc. [0003] Although the above-mentioned methods are commonly used methods in the prior art, they all have their own advantages and disadvantages...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/02
CPCA01K67/02A01K2207/30A01K2207/20A01K2227/105A01K2267/03
Inventor 李树民孙影杨方徐洪王朋李雅倩张乙
Owner NORTH CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY
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