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Method for detecting tyramine content in aquatic products based on azo coupling reaction and surface enhanced resonance Raman scattering

A technology of surface enhancement and Raman scattering, applied in Raman scattering, preparation of test samples, measuring devices, etc., can solve the problems of undisclosed tyramide and achieve good repeatability, accuracy, and high sensitivity

Inactive Publication Date: 2019-12-27
NINGBO UNIV
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At present, there are no relevant research reports on the method of detecting tyramine content in aquatic products based on azo coupling reaction and surface-enhanced resonance Raman scattering.

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  • Method for detecting tyramine content in aquatic products based on azo coupling reaction and surface enhanced resonance Raman scattering
  • Method for detecting tyramine content in aquatic products based on azo coupling reaction and surface enhanced resonance Raman scattering
  • Method for detecting tyramine content in aquatic products based on azo coupling reaction and surface enhanced resonance Raman scattering

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[0035] The present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.

[0036] 1. Specific implementation

[0037] 1. Materials and instruments

[0038] Materials: Fresh mackerel and salted fish were purchased from Ningbo Lulin Market. After the samples were purchased, they were stored at -80°C for later use. Remove the fish skin and bones, and only keep the back muscles as experimental materials.

[0039] Reagent: p-aminobenzoic acid (PABA, C 6 h 7 NO 3 S, ≥ 99.8 %), silver nitrate (AgNO 3 ,, 99.9%), sodium nitrite (NaNO 2 , ≥ 99 %), hydrochloric acid (HCl, 36.46%), sodium carbonate (Na 2 CO 3 , ≥ 99.8%) and sodium citrate (98%) were purchased from Sinopharm Chemicals Co., Ltd. (Shanghai, China). Tyramine hydrochloride (C 8 h 11 NO·HCl, 98%), chloroauric acid (HAuCl 4 , 98%), L-ascorbic acid (99%) and 4-mercaptobenzoic acid (4MBA, 99%) were purchased from Sigma-Aldrich (Shanghai, China). Deionized w...

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Abstract

The invention discloses a method for detecting tyramine content in aquatic products based on the azo coupling reaction and the surface enhanced resonance Raman scattering. The method for detecting tyramine content in aquatic products based on the azo coupling reaction and the surface enhanced resonance Raman scattering is characterized by comprising the following steps: taking fresh fish meat andadding trichloroacetic acid solution for homogenate, shaking at room temperature, then centrifuging to take the supernatant, repeatedly extracting the residue with the trichloroacetic acid solution, merging the supernatant to make up the volume, and passing a filtering membrane, so as to obtain a sample solution to be detected; adding a NaNO<2> solution to a mixture obtained by dissolving para aminobenzoic acid in a HCl solution, and quickly adding a Na<2>CO<3> solution and a tyramine solution to carry out the tyramine azo coupling reaction under a stirring condition after the reaction, so asto obtain a tyramine-derived azo compound solution; and mixing the tyramine-derived azo compound solution with Au@Ag NPs solution in proportion to perform spectral detection, and calculating the tyramine content in the sample to be detected according to the linear relationship between the tyramine concentration and the characteristic peak signal intensity. The advantages of the method for detecting tyramine content in aquatic products based on the azo coupling reaction and the surface enhanced resonance Raman scattering are simple, fast and high-sensitivity.

Description

technical field [0001] The invention relates to a method for detecting tyramine content in aquatic products, in particular to a method for detecting tyramine content in aquatic products based on azo coupling reaction and surface-enhanced resonance Raman scattering. Background technique [0002] Tyramine (1-hydroxy-4-ethylaminobenzene) is a kind of biogenic amine, which is a microbial active degradation product. Found in fermented foods, beverages, meat, fish, seafood and dairy products. High-concentration tyramine is extremely harmful to human health. It can promote peripheral vasoconstriction, stimulate heart rhythm, increase blood sugar concentration, eliminate norepinephrine in the nervous system, and cause migraine. Therefore, it is very important to establish a rapid and sensitive method for the determination of tyramine. At present, a variety of methods have been established at home and abroad to quantitatively determine the content of tyramine in various foods, incl...

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Application Information

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IPC IPC(8): G01N21/65G01N1/28G01N1/40G01N33/12
CPCG01N1/28G01N1/4077G01N21/658G01N33/12
Inventor 张进杰郭小莹黄栋玮杨君萍俞青瑶徐大伦
Owner NINGBO UNIV
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