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thermostable xylanase mutant

A technology of xylanase mutation and xylanase, which is applied in the field of protein engineering and can solve the problems that cannot meet the needs of industrial production

Active Publication Date: 2022-05-31
QINGDAO VLAND BIOTECH GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Although a lot of research has improved the stability of xylanase, it still cannot meet the increasing demand for industrial production. Therefore, it is of great practical significance to provide a high-temperature resistant xylanase suitable for industrial production.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Take out 30ul of lysate to two new 96-well plates respectively; one of them is processed at 75°C for 8min; then the two

[0066] The nucleotide sequence of the above-mentioned xylanase mutant was synthesized by Shanghai Jierui Biological Company.

Embodiment 3

[0074] Release 1 μmol per minute from a xylan solution at a concentration of 5 mg / ml at 37° C. and pH 5.5

[0078]

Embodiment 4

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Abstract

The present invention provides a heat-resistant xylanase mutant, wherein the amino acid at the 28th and / or 37th position of the xylanase with the amino acid sequence of SEQ ID NO: 1 is substituted. The xylanase mutants Xyn1-28, Xyn1-37 and Xyn1-28 / 37 provided by the present invention have significantly improved heat resistance. Compared with xylanase Xyn1, after treatment at 80°C for 5 minutes, the residual rate of enzyme activity of the three xylanase mutants generally increased by 18.83%-33.91%; after treatment at 85°C for 3 minutes, the residual rate of enzyme activity generally increased It has increased by 25.50%‑74.87%, and has achieved unexpected technical effects. The xylanase mutant has high heat resistance, is beneficial to its application in feed, and has broad market prospects.

Description

Thermostable xylanase mutant technical field The invention belongs to the technical field of protein engineering, be specifically related to a kind of xylopolymer obtained by heat resistance screening Carbohydrase mutants. Background technique Xylan is the main component of plant hemicellulose, widely exists in corncob, bagasse, wheat bran, straw In crop wastes such as xylanase, xylanase can decompose xylan into xylo-oligosaccharides and xylose of different lengths. These resources have important economic value, and these available resources can be fully utilized through xylanase to give full play to their potential application value. value, the study of xylanase has also received sufficient attention. Xylanase is a glycosyl hydrolase that hydrolyzes β-1,4-linked xylopyranoside chains and has been shown to exist in at least Hundreds of different organisms and can be produced economically on a large scale. Together with other glycosyl hydrolases, they form A su...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/42C12N15/56C12N15/81C12N1/19C12R1/84
CPCC12N9/2482C12N15/815C12Y302/01008Y02P60/87
Inventor 吴秀秀邵弨李瑞黄亦钧
Owner QINGDAO VLAND BIOTECH GRP
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