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Methylobacterium sp., and isolated culture method and application thereof

A methylobacillus, isolation and culture technology, applied in the directions of microorganism-based methods, isolation of microorganisms, biochemical equipment and methods, etc., can solve problems such as carcinogens

Active Publication Date: 2019-12-24
CHINA TOBACCO HENAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a strain of Methylobacterium, method of isolation and cultivation and application thereof, to solve the problem that sterols in tobacco produce carcinogens after pyrolysis at high temperature

Method used

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  • Methylobacterium sp., and isolated culture method and application thereof
  • Methylobacterium sp., and isolated culture method and application thereof
  • Methylobacterium sp., and isolated culture method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0038] 1. Culture medium preparation:

[0039] Stigmasterol mother liquor: Add 25 g of stigmasterol to 1 L of water, add 2 ml of DMSO (dimethyl sulfoxide), sterilize after sonication, and store in a refrigerator at 4°C.

[0040] Tobacco medium solid plate: take 20g of tobacco stems or leaves, add 400ml of water to extract at 65°C for 1 hour, filter to remove solids to obtain a liquid medium, add 4g of agar, sterilize with high pressure steam at 121°C for 20min, and cool down to prepare Obtain solid media plates.

[0041] Seed medium solid plate with sterol as the only carbon source: 25g / L stigmasterol mother liquor: 160ml, citric acid: 2g, ferric ammonium citrate: 0.05g, dipotassium hydrogen phosphate: 0.5g, anhydrous magnesium sulfate: 0.5g , ammonium nitrate: 32g, deionized water was added to 1L, agar 30g. pH 7.5, sterilized by high-pressure steam at 121°C for 20 minutes, and cooled to prepare seed culture medium.

[0042] Seed medium: glucose: 5.0g, glycerin: 20g, citric...

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Abstract

The invention relates to a methylobacterium sp., and an isolated culture method and application thereof, and the methylobacterium sp. WGM 16 is preserved in China Center for Type Culture Collection with the preservation number of CCTCC NO: M2019336. According to the technical scheme, a bacterial strain is obtained by separating tobacco sewage, conducting enriching by using sterol mother liquor, respectively conducting culturing by using a tobacco culture medium containing sterol as a carbon source and a seed culture medium taking sterol as a unique carbon source, and separating bacterial colonies with specific colors. By utilizing the bacterial strain, the sterol content in tobacco products can be effectively reduced, so that the content of harmful substances formed by sterol substances ina combustion process is reduced, and the purposes of reducing tar and harm of the tobacco products are achieved.

Description

technical field [0001] The invention belongs to the technical field of microbial strains, and in particular relates to a new microbial strain cultivated and separated by using tobacco sewage and its application. Background technique [0002] Tobacco contains about 0.3% phytosterols. Studies have shown that about 20-25% of tobacco phytosterols are completely transferred into the smoke and cause harm through thermal cracking. Sterols in tobacco can produce carcinogenic fused-ring aromatic hydrocarbons after high-temperature cracking, benzopyrene is the most typical, and these harmful substances can enter mainstream smoke. And then absorbed by the human body, thus causing harm to human health. Therefore, using appropriate biological methods to degrade sterols in tobacco into small molecular organic acids or eventually into CO 2 And water, it is expected to improve the sensory quality of tobacco and reduce the harm of tobacco, which has important application value and researc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02C12R1/01
CPCC12N1/20C12N1/02C12R2001/01C12N1/205
Inventor 张展叶建斌王璐陈小龙郭霖轩王金棒杨宗灿冯颖杰张婷婷杨晨李倩
Owner CHINA TOBACCO HENAN IND
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