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DNA bar code and method for quick identification of nightshade

A nightshade and barcoding technology, applied in the field of molecular biology, can solve problems such as the lack of DNA barcoding to identify nightshade plants, and achieve the effects of facilitating and accurate identification of medicinal ingredients, combating mixed adulteration, and simplifying identification procedures.

Pending Publication Date: 2019-12-20
VEGETABLE & FLOWER INST JIANGXI ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the chloroplast DNA sequence fragments matK and psbA-trnH have been widely used in the classification and identification of plants, but there is no report on the identification of Solanum plants using DNA barcodes

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Establishment of a method for identifying Solanum species using the DNA barcode matK sequence

[0036]The matK sequence fragments of 58 Solanum species were retrieved from the NCBI database, including Solanum acaule, S.aculeastrum, S.aculeatissimum, S.aethiopicum, S.agnewiorum, S.amotapense, S.anguivi, S.argenteum, S. aureitomentosum, S. aviculare, S. berthaultii, S. brevicaule, S. bulbocastanum, S. campylacanthum, S. cerasiferum, S. chacoense, S. cheesmaniae, S. chilense, S. coagulans, S. commersonii, S. dasyphyllum, S. demissum, S. dulcamara, S. elaeagnifolium, S. galapagense, S. habrochaites, S. hougasii, S. lanzae, S. lichtensteinii, S. linnaeanum, S. lycopersicum, S. lyratum, S. macrocarpon, S. mammosum, S. melongena, S. neorickii, S. nigrum, S. pennelii, S. peruvianum, S. pimpinellifolium, S. polhillii, S. pseudocapsicum, S. richardii, S. rigidum, S. sisymbriifolium, S. spirale, S.stloniferum, S.stupefactum, S.supinum, S.torvum, S.trilobatum, S.tuberosu...

Embodiment 2

[0037] Example 2 Establishment of a method for identifying Solanum species using the DNA barcode psbA-trnH sequence

[0038] The psbA-trnH sequence fragments of 49 Solanum species were retrieved from the NCBI database, including S.acaule, S.aculeastrum, S.aculeatissimum, S.aethiopicum, S.agnewiorum, S.anguivi, S.aureitomentosum, and S.aviculare , S.berthaultii, S.brevicaule, S.bulbocastanum, S.campylacanthum, S.cerasiferum, S.cheesmaniae, S.chilense, S.coagulans, S.commersonii, S.dasyphyllum, S.dulcamara, S.glabratum, S. .elaeagnifolium, S.lanzae, S.lichtensteinii, S.linnaeanum, S.lycopersicum, S.lyratum, S.macrocarpon, S.melongena, S.neorickii, S.nigrum, S.peruvianum, S.pimpinellifolium, S.polhillii , S. pseudocapsicum, S. richardii, S. rigidum, S. spirale, S. stoloniferum, S. stupefactum, S. supinum, S. torvum, S. trilobatum, S. tuberosum, S. umtuma, S. usambarense, S. 218 sequences including 49 species including .villosum, S.violaceum, S.virginianum and S.wrightii, each se...

Embodiment 3

[0039] Embodiment 3 identifies the making of nightshade kit and comparison card

[0040] Based on the above two examples, production and identification of Solanum plant water eggplant (Solanum torvum), garlic mustard eggplant (S.sisymbriifolium), eggplant (S.melongena), white English (S.lyratum), caleatissimum (S.aculeatissimum) ), coral cherry (S.pseudocapsicum) and S.campylacanthum kits and Solanum species comparison card MB.

[0041] The kit includes 4 primers whose sequences are SEQ ID NO.3-SEQ ID NO.6, and conventional PCR reagents.

[0042] "Solanum species comparison card MB" is as follows:

[0043] If the matK sequence of the plant to be tested is compared with SEQ ID NO.1, the 491st base is C, and the 726th base is T, then the material to be tested is cultivated eggplant (Solanummelongena);

[0044] If the matK sequence of the plant to be tested is compared with SEQ ID NO.1, and the 682nd base is T, then the material to be tested is Solanumtorvum;

[0045] If the m...

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PUM

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Abstract

The invention relates to a DNA bar code and method for quick identification of nightshade. A conservative primer of a DNA bar code matK or psbA-trnH is used for performing PCR amplification and sequencing on genomic DNA, according to a specific site base sequence, species identification can be performed, and solanum melongena, solanum torvum, solanum sisymbriifolium, solanum lyratum, solanum aculeatissimum, solanum pseudocapsicum and S.campylacanthum can be identified. According to the DNA bar code disclosed by the invention, the identification result can be directly obtained through a matK orpsbA-trnH sequence, the detection method is quick, simple, convenient and accurate, and the DNA bar code can be applied to the respect of identification, protection and utilization of solanum germplasm resources, quarantine of entry and exit plants and products thereof, and identification of relevant solanum medicinal material adulterants and counterfeits.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to a DNA barcode and a method for rapid identification of plants of the genus Solanum. Background technique [0002] There are more than 2,000 species of Solanum in the world, and it is the largest genus of Solanaceae (Solanaceae), mostly distributed in tropical and subtropical regions. The genus Solanum includes Solanum melongena, the second largest economic crop of Solanaceae, 22 kinds of medicinal plants and ornamental plants. Eggplant contains saponin, vitamin P, calcium, phosphorus and other nutrients, and is widely planted all over the world. It is an important vegetable crop in Asia, the Middle East, the Near East, Southern Europe and Africa; there are many medicinal plants in the nightshade genus, such as Bai Ying (S .lyratum), Solanum nigrum (S.nigrum), Caleatissimum (S.aculeatissimum, Syn.S.khasianum), coral cherry (S.pseudocapsicum) and water eggplant (S.torvum) are all ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156
Inventor 袁欣捷方荣陈学军周坤华雷刚黄月琴
Owner VEGETABLE & FLOWER INST JIANGXI ACADEMY OF AGRI SCI
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