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Purification method of albumin in dog serum

A purification method and blood albumin technology, applied in the field of biological products, can solve the problems of complicated operation, many steps, waste of resources and cost, etc., and achieve the effects of simple process method, high-purity recovery rate, and resource saving

Active Publication Date: 2019-12-20
CHANGCHUN SR BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the purification process of human serum albumin, the five-step or seven-step method of low-temperature ethanol is generally used to separate and purify various components in human plasma step by step. The operation is cumbersome, there are many steps, the consumption of reagents is large, and the cost is high. Although we can Learn from the purification process of human serum albumin, but albumin and globulin in canine serum are mainly used in pharmaceuticals, and other components have not yet been formed into products. Separation will result in a waste of resources and an increase in cost, and the separation and purification method of human serum albumin cannot be applied to the separation and purification of canine serum albumin, and it is not conducive to the marketization of canine serum albumin

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  • Purification method of albumin in dog serum
  • Purification method of albumin in dog serum
  • Purification method of albumin in dog serum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1480

[0038]Example 1 Purification of canine serum albumin in 480 bottles of canine serum in two steps

[0039] 1. Materials

[0040] Canine serum (the content of albumin in serum is 25g / L), sodium chloride, sodium octanoate, glacial acetic acid, sodium acetate

[0041] 2. Method

[0042] Place 480 bottles of canine serum (500ml / bottle) in a melting room (10°C) and place them at intervals of 10cm to melt until only ice cubes with a diameter of about 3cm are left in the bottle. Pour the canine serum and ice cubes together In a small stainless steel bucket, after 16000rpm tubular centrifugation, filter the canine serum with a 0.45μm column core to remove residual cells and insoluble protein components; weigh the clarified canine serum, then place it in a -10°C freezer, install a stirrer, Stir at a low speed, adjust the pH value to 7.0 with acetic acid-sodium acetate buffer solution with a pH value of 4.0, then slowly add 95% ethanol at -20°C during the stirring process, the dosage i...

Embodiment 2240

[0047] Example 2 Purification of canine serum albumin with 240 bottles of canine serum in two steps

[0048] 1. Materials

[0049] Canine serum (the content of albumin in serum is 25g / L), sodium chloride, sodium octanoate, glacial acetic acid, sodium acetate

[0050] 2. Method

[0051] Place 240 bottles of canine serum (500ml / bottle) in a melting room (12°C) and place them at intervals of 10cm to melt until only ice cubes with a diameter of about 3cm are left in the bottle. Pour the canine serum and ice cubes together In a small stainless steel bucket, after 16000rpm tubular centrifugation, filter the canine serum with a 0.45μm column core to remove residual cells and insoluble protein components; weigh the clarified canine serum, then place it in a -12°C freezer, install a stirrer, Stir at a low speed, adjust the pH value to 6.8 with acetic acid-sodium acetate buffer solution with a pH value of 4.0, and then slowly add 95% ethanol at -25°C during the stirring process, the d...

Embodiment 3480

[0056] Example 3 Purification of canine serum albumin in 480 bottles of canine serum in two steps

[0057] 1. Materials

[0058] Canine serum (the content of albumin in serum is 25g / L), sodium chloride, sodium octanoate, glacial acetic acid, sodium acetate

[0059] 2. Method

[0060] Place 480 bottles of canine serum (500ml / bottle) in a melting room (20°C) at an interval of 10cm to melt until only ice cubes with a diameter of about 3cm are left in the bottle. Pour the canine serum and ice cubes together In a small stainless steel bucket, after 16000rpm tubular centrifugation, filter the canine serum with a 0.45μm column core to remove residual cells and insoluble protein components; weigh the clarified canine serum, then place it in a -8°C freezer, install a stirrer, Stir at a low speed, adjust the pH value to 7.2 with acetic acid-sodium acetate buffer solution with a pH value of 4.0, and then slowly add 95% ethanol at -23°C during the stirring process, the dosage is 25% of ...

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Abstract

The invention discloses a purification method of albumin in dog serum. By an ethanol two-step method, albumin is purified from the dog serum, wherein in a step S1, a cold-temperature ethanol method ofwhich the temperature is less than or equal to minus 20 DEG C is used, and in a step S2, a thermal ethanol method of which the temperature is 65-68 DEG C is adopted. According to the purification method of albumin in dog serum provided by the invention, high-purity dog blood albumin can be obtained by the ethanol two-step method, the purification method is a quick simple high-purity high-recovery-rate technological method for purifying the albumin from the dog serum, can be applied to preparation of dog albumin medicines and medicines for treating dog relevant diseases, and is simple in technological method, low in cost and suitable for large-scale production, and resources are saved.

Description

technical field [0001] The invention relates to the technical field of biological products, in particular to a method for purifying albumin in canine serum. Background technique [0002] With economic development and people's life becoming more and more prosperous, more and more people keep pets to accompany themselves and their families. Dogs are the most numerous pet species. According to incomplete statistics, there are as many as 120 million dogs in my country. With the increasing number of dogs, the occurrence of canine diseases is inevitable. The use of canine albumin in the treatment of canine diseases is common and effective. The case of using serum as canine albumin. [0003] In the purification process of human serum albumin, the five-step or seven-step method of low-temperature ethanol is generally used to separate and purify various components in human plasma step by step. The operation is cumbersome, there are many steps, the consumption of reagents is large, ...

Claims

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Application Information

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IPC IPC(8): C07K14/765C07K1/14
CPCC07K14/765
Inventor 石晶赵健陈宪平陈放付玉侯强董鹏殷玉和
Owner CHANGCHUN SR BIOLOGICAL TECH
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