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Eucalyptus polybractea essential oil and application of composite microbial agent of eucalyptus polybractea essential oil in preparation of crustacean insect repellents

A technology of crustaceans and composite bacterial agents, which is applied to crustacean insect repellents. In the field of eucalyptus eucalyptus essential oil and its composite bacterial agents, it can solve the problem of damage to the health of aquatic animals, unstable drug dosage, and problems caused by consumers. Potential safety hazards and other issues, to achieve the effect of reducing bacterial infection, saving breeding costs, and a wide range of applications

Active Publication Date: 2019-12-20
SHANDONG LONGCHANG ANIMAL HEALTH PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in order to simply pursue immediate results, traditional medicines will cause excessive heavy metals and unstable drug dosages, which will destroy the stability of water quality, damage the health of aquatic animals and reduce their immunity. In severe cases, it will directly lead to death. , ultimately reducing production efficiency and bringing safety hazards to consumers

Method used

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  • Eucalyptus polybractea essential oil and application of composite microbial agent of eucalyptus polybractea essential oil in preparation of crustacean insect repellents
  • Eucalyptus polybractea essential oil and application of composite microbial agent of eucalyptus polybractea essential oil in preparation of crustacean insect repellents
  • Eucalyptus polybractea essential oil and application of composite microbial agent of eucalyptus polybractea essential oil in preparation of crustacean insect repellents

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] One, the preparation of Eucalyptus eucalyptus essential oil

[0030] The preparation process of Eucalyptus eucalyptus essential oil in the present invention is as figure 1 As shown, first put the leaves of Eucalyptus eucalyptus in Australia into the washing tower for cleaning, put them in the dryer for drying, and then pulverize them in the pulverizer and then enter the steam distillation kettle, condenser and oil-water stratifier for separation The oil phase and the water phase, the water phase enters the ether extractor to separate the waste water, the liquid containing ether enters the ether recovery tower to recover ether, and the recovered ether and oil phase liquid are added to the solid bed adsorption dryer to further remove the ether in the oil phase Finally, the oil phase liquid is added to a vacuum rectification tower for rectification to remove impurity oils to obtain eucalyptus multibract essential oil.

[0031] The essential oil of Eucalyptus multibract pr...

Embodiment 2

[0041] Penaeus vannamei was cultured in Waitang, Sheyang, Jiangsu, with 35,000 tails / mu. In mid-May, the shrimps showed slow growth, small food intake, opaque abdomen, empty jejunum, and blurred hepatopancreas. Typical symptoms of microsporidia in prawns. 1 kg of eucalyptus eucalyptus essential oil prepared in Example 1 was used to mix 1 ton of feed, and it was carried out according to normal feeding and management, and fed continuously for 7 days, 3 times a day. After 7 days, check the condition of the prawns again. As shown in Figure 4, at the beginning of feeding, the large abdomen is opaque, the jejunum is empty, the hepatopancreas is blurred, and the typical symptoms of microsporidia; after 7 days of feeding, there is food in the intestines and stomach, the hepatopancreas becomes clear, and the body color turns clear. It shows that the essential oil of eucalyptus eucalyptus has obvious anthelmintic effect on Penaeus vannamei.

Embodiment 3

[0043] Portunus crabs were bred in Ningbo, Zhejiang Province. Small, wriggling, reddish parasites were found on the bottom of the crab fins before the crabs matured. Microscopic examination confirmed that they were lung flukes. Immediately adopt 1 kg of eucalyptus eucalyptus essential oil compound bacterial agent prepared by the present invention to mix with 1 ton of portunus feed, and carry out by normal feeding and management, and feed continuously for 10 days, 3 times every day. After 10 days, check the status of swimming crabs again. The results showed that at the beginning of feeding, small red lung flukes appeared on the bottom of swimming crab fins; after 10 days of feeding, the lung flukes on the bottom of swimming crab fins disappeared. .

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Abstract

The invention provides eucalyptus polybractea essential oil and application of a composite microbial agent of the eucalyptus polybractea essential oil in preparation of crustacean insect repellents. The eucalyptus polybractea essential oil composite microbial agent is prepared from eucalyptus polybractea essential oil and streptomyces griseus HL-50 of which the bacterial content is not less than 5x10<8> CFU / g, wherein the preservation number of the streptomyces griseus HL-50 is CGMCC No.18424, and the most main effective component of theeucalyptus polybractea essential oil is 1,8-cineole. Theeucalyptus polybractea essential oil composite microbial agent is simple to prepare and use, and is uniformly mixed with an edible feed for marine fishes just according to a dosage of 0.5-1.5 kg / ton,and the marine fishes are continuously fed for 4-10 days so as to achieve the effects that: the various parasites infecting crustaceans can be effectively repelled and bacteria can be inhibited. The composite microbial agent is non-toxic and harmless, is suitable for long-term use of crustaceans, reduces the breeding cost, and has wide application prospects.

Description

technical field [0001] The invention belongs to the technical field of aquaculture, and in particular relates to the application of eucalyptus eucalyptus eucalyptus essential oil and its composite bacterial agent in the preparation of an insect repellant for crustaceans. Background technique [0002] Essential oils are volatile aromatic substances extracted from the flowers, leaves, stems, roots or fruits of plants through steam distillation, extrusion, cold soaking or solvent extraction, most of which have a lighter specific gravity than water. The relative density is generally in the range of 0.8 to 1.17. Essential oils are sensitive to heat and light, so they need to be stored in dark containers and in a cool place, and essential oils are now mostly used in skin care products and aromatherapy because of their special aroma and the characteristics of being soluble in organic solvents, but the function of plant essential oils Various, in addition to the traditional fragran...

Claims

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Application Information

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IPC IPC(8): A23K50/80A23K10/30A23K10/18A23K20/158A61K36/61A61P33/00A61P31/04A01K61/59
CPCA23K50/80A23K10/30A23K10/18A23K20/158A61K36/61A61P33/00A61P31/04A01K61/59Y02A50/30
Inventor 张西雷籍立民娄倩倩
Owner SHANDONG LONGCHANG ANIMAL HEALTH PROD
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