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Liquid absorption mechanism and working method thereof

A pipette and liquid technology, applied in biochemical equipment and methods, biological material sampling methods, biochemical instruments, etc., can solve the problems of environmental cross-contamination, inability to separate cleanly, and reduce the success rate of cell culture, and achieve accurate separation high rate effect

Pending Publication Date: 2019-12-13
CELLAUTO BIOLOGICAL AUTOMATION CO LTD +1
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  • Abstract
  • Description
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Problems solved by technology

[0003] At present, in the manual or automated stem cell preparation process, the supernatant is taken out by the operator manually pouring the liquid quickly, which has a low storage efficiency, and some cells are prone to flow out with the supernatant during the operation. Or, the colloidal dispersion system formed by dispersing and suspending the small particles of the supernatant in the gas medium will cause cross-contamination to the environment, or during the process of pouring the liquid, some of the liquid will stick to the wall of the culture dish and cannot be separated cleanly, thus causing the cell Reduced success rate

Method used

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  • Liquid absorption mechanism and working method thereof

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Embodiment Construction

[0025] In order to make the purpose, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0026] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative efforts fall within the protection scope of the present invention.

[0027] In describing the present invention, it should be understood that the terms "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", " Orientation or position indicated by "back", "left", "...

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PUM

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Abstract

The invention relates to a liquid absorption mechanism and a working method thereof. The mechanism comprises a bracket, a centrifuge bottle, a control chip, a liquid absorption component and a visualinspection component, wherein the visual inspection component comprises a camera component and a visual controller; the camera component and the visual controller are connected; the visual controllerand the liquid absorption component are independently connected with the control chip; the centrifuge bottle, the camera component and the liquid absorption component are connected to a bracket; the photo of the centrifuge bottle is shot through the camera component; and after the visual controller carries out photo analysis, liquid layering position information is obtained, the liquid layering position information is sent to the control chip, and the control chip drives the liquid absorption component to carry out an absorption operation of an upper-layer clear liquid in the centrifuge bottle. By use of the liquid absorption mechanism disclosed by the invention, the fully automatic absorption of the upper-layer clear liquid is realized, and in addition, liquid separation accuracy is highby visual identification and inspection.

Description

technical field [0001] The invention relates to a liquid suction mechanism, more specifically to a liquid suction mechanism and its working method. Background technique [0002] In the process of cell preparation, the liquid of the transferred cells needs to be centrifuged, and the cultured stem cells are stripped from the culture dish, which contains both stem cells and culture fluid, and after being separated by a centrifuge, the cells are precipitated on the centrifugal screen. At the bottom, the centrifuged supernatant needs to be separated from the centrifuge bottle for cell culture. [0003] At present, in the manual or automated stem cell preparation process, the supernatant is taken out by the operator manually pouring the liquid quickly, which has a low storage efficiency, and some cells are prone to flow out with the supernatant during the operation. Or, the colloidal dispersion system formed by dispersing and suspending the small particles of the supernatant in t...

Claims

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Application Information

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IPC IPC(8): C12M1/26C12M1/24C12Q1/24
CPCC12M23/08C12M33/04C12Q1/24
Inventor 刘沐芸李晓智杜祥熙李树盛
Owner CELLAUTO BIOLOGICAL AUTOMATION CO LTD
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