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Eucalyptus polybractea essential oil with anthelmintic effect, and composite microbial agent and application thereof

A technology of insect repellent and compound bacterial agent, which is applied in the direction of application, pest repellent, animal repellent, etc., can solve the problems of damage to livestock and poultry animal health, unstable drug dosage, hidden dangers of consumer safety, etc., and achieve reduction Bacterial infection, saving cost of breeding, wide range of effects

Active Publication Date: 2019-12-13
SHANDONG LONGCHANG ANIMAL HEALTH PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in order to simply pursue immediate results, traditional medicines will cause excessive heavy metals and unstable drug dosage, which will damage the environment, damage the health of livestock and poultry animals, reduce their immunity, and even directly lead to death. Finally, the production efficiency is reduced, and it also brings safety hazards to consumers.

Method used

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  • Eucalyptus polybractea essential oil with anthelmintic effect, and composite microbial agent and application thereof
  • Eucalyptus polybractea essential oil with anthelmintic effect, and composite microbial agent and application thereof
  • Eucalyptus polybractea essential oil with anthelmintic effect, and composite microbial agent and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The preparation process of Eucalyptus eucalyptus essential oil in the present invention is as figure 1 As shown, first put the leaves of Eucalyptus eucalyptus in Australia into a washing tower for cleaning, put them in a dryer for drying at 180°C, and then crush them in a pulverizer to a size of 10 meshes, then put them into a steam distillation kettle and add water , heating, steam and oil gas enter the condenser to condense, the condenser and the oil-water stratifier separate the oil phase and the water phase, and the water phase enters the ether extractor to separate the waste water, and the liquid containing ether enters the ether recovery tower to recover ether. Add the recovered ether and oil phase liquid to the solid bed adsorption dryer to further remove the water in the oil phase, and finally add the oil phase liquid to the vacuum distillation tower for rectification to remove impurity oil and obtain Eucalyptus multibract essential oils. 1 kg of eucalyptus lea...

Embodiment 2

[0033] 1. Screening of Streptomyces griseus HL-50

[0034] Take the soil as a sample, mix the soil and sterile water to make 10% of the soil -1 diluent, and then prepare 10 -2 、10 -3 、10 -4 、10 -5 Diluent. Take 10 respectively -3 、10 -4 、10 -5 The diluted solution was spread on Gaoshi Synthetic No. 1 agar medium, cultured at 30°C for 7 days, and the obvious colonies were selected for multiple purifications to obtain a single colony, which was named HL-50, and placed on the slope of Gaoshi Synthetic No. 1 agar. stored in culture medium. The formula of described Gao's synthetic No. 1 agar medium is: KNO 3 1g, 20g soluble starch, K 2 HPO 4 0.5g, MgSO 4 ·7H 2 O 0.5g, NaCl 0.5g, FeSO 4 0.01g, 20g agar, 1000mL distilled water, pH 7.2. Such as image 3 As shown, the biological characteristics of the colony of the bacterial strain HL-50 on Gaoshi Synthetic No. 1 agar medium are: the colony is round, with a small area, a slight protrusion in the middle, light khaki, ...

Embodiment 3

[0041] Aiming at the limitations of chemical medicines in the current deworming aspect, the limited use on sows and safety issues, this example uses the eucalyptus eucalyptus essential oil compound bacterial agent prepared in Example 2 for deworming in sows experiment.

[0042] Test object: a pig farm in Zaozhuang, with 100 sows in stock, with serious body mites.

[0043] Experimental plan: select 6 sows, use eucalyptus eucalyptus essential oil compound bacterial agent to deworm the body surface, and spray the compound bacterial agent, record the experimental process, including the individual reaction of the experiment, the amount of pouring, etc., and continue to follow up, 10 Divas comparison. The test results are shown in Figure 4: about 7-10 days after the 40-50ml of the Eucalyptus eucalyptus essential oil compound bacterial agent was applied on the body surface of each sow, it can be found that the old skin caused by parasites began to fall off, and the pigs The body be...

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Abstract

The invention discloses a Eucalyptus polybractea essential oil with an anthelmintic effect, and a composite microbial agent and an application thereof. The Eucalyptus polybractea essential oil includes 1,8-cineole, beta-methylisopropylbenzene, limonene, alpha-pinene and alloaromadendrene, wherein the most important effective component of the Eucalyptus polybractea essential oil is 1,8-cineole. Theinvention also discloses the composite microbial agent prepared from the Eucalyptus polybractea essential oil and Streptomyces griseus HL-50 with the preservation number of CGMCC No.18424. The composite microbial agent can be applied to livestock and poultry breeding in order to effectively repel various parasites infecting livestock and poultry and inhibit bacteria. The essential oil has the advantages of natural components, excellent quality, and no influences on feeding and growth of animals, and the composite microbial agent also has the advantages of simplicity in preparation, safety inuse, no toxic or side effects, improvement of the growth performance and the intestinal health of livestock and poultry, reduction of the breeding cost, and wide application prospect.

Description

technical field [0001] The invention belongs to the technical field of essential oils in biology, and in particular relates to an essential oil of eucalyptus eucalyptus with repellent effect and its compound bacterial agent and its application. Background technique [0002] Essential oils are volatile aromatic substances extracted from the flowers, leaves, stems, roots or fruits of plants through steam distillation, extrusion, cold soaking or solvent extraction, most of which have a lighter specific gravity than water. The relative density is generally in the range of 0.8 to 1.17. Essential oils are sensitive to heat and light, so they need to be stored in dark containers and in a cool place, and essential oils are now mostly used in skin care products and aromatherapy because of their special aroma and the characteristics of being soluble in organic solvents, but the function of plant essential oils Various, in addition to the traditional fragrance, soothing and invigorati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/90A01N35/02A01N31/06A01N27/00A01N35/06A01N31/04A01N63/00A01P1/00A01P17/00A61K31/015A61K31/045A61K31/121A61K31/122A61K31/35A61K35/74A61P33/10A61P33/14A61P33/02
CPCA01N27/00A01N31/04A01N31/06A01N35/02A01N35/06A01N43/90A01N63/00A01N65/28A61K31/015A61K31/045A61K31/121A61K31/122A61K31/35A61K35/74A61K36/61A61P33/02A61P33/10A61P33/14A61K2300/00
Inventor 高志强籍立民娄倩倩
Owner SHANDONG LONGCHANG ANIMAL HEALTH PROD
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