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Cell penetration enhancing peptides, compositions and applications thereof

A penetration-enhancing, cellular technology, applied in peptides, cosmetic preparations, pharmaceutical formulations, etc., can solve problems such as lack of predictability

Active Publication Date: 2020-11-13
HARVEST BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] It can be seen that the prior art has proposed a transdermal scheme composed of about 10-11 amino acids that can enhance the functional ingredients of active macromolecules. However, the inventors have found through a large number of experimental studies that when the amino acid sequence is changed, the macromolecular penetration is promoted. The effect of the skin will also change greatly, lacking predictability

Method used

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  • Cell penetration enhancing peptides, compositions and applications thereof
  • Cell penetration enhancing peptides, compositions and applications thereof
  • Cell penetration enhancing peptides, compositions and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Embodiment 1: the detection of polypeptide factor total protein content (BCA method)

[0048] 1. Drawing of the standard curve: Take an ELISA plate and dilute the equal ratio reagents according to the instruction manual for the drawing of the following standard curve;

[0049] 2. According to the number of samples, prepare an appropriate amount of BCA working solution according to 50 volumes of BCA reagent A plus 1 volume of BCA reagent B (50:1), and mix well;

[0050] 3. Add 200 μL BCA working solution to each well;

[0051] 4. Put the ELISA plate on the shaker for 30 sec, place it at 37°C for 30 minutes, and then measure it colorimetrically at 562nm. Draw the standard curve with the protein content (μg) as the abscissa and the absorbance value as the ordinate;

[0052] 5. Dilute the sample to be tested to an appropriate concentration, so that the total volume of the sample diluent is 20 μL, add 200 μL of BCA working solution, mix well, and place it at 37°C for 30 mi...

Embodiment 2

[0054] Example 2: ELISA detection of key cytokines of polypeptide factors before and after transdermal (ELISA method)

[0055] For the detection of the relative concentration of human EGF factor, human PDGF factor and HGF factor, Wuhan Boster Biotech’s human EGF ELISA Kit (EK0325), human PDGF-AB ELISA Kit (EK0484) and human HGF ELISA Kit (EK0369) were used respectively. The steps are briefly described as follows:

[0056] 1. Collect the culture medium (i.e., polypeptide factor) after two days of culture of the mesenchymal stem cells and the empty culture medium control, and centrifuge to remove cell debris. Each group has 6 techniques in parallel.

[0057] 2. Add 100 μl of sample and blank control to each well, and react at 37°C for 90 minutes without washing.

[0058] 3. Add 100 μl of biotin-labeled antibody to each well and react at 37°C for 60 minutes.

[0059] 4. Wash 3 times with 0.01M TBS.

[0060] 5. Add 100 μl ABC to each well and react at 37°C for 30 minutes.

[...

Embodiment 3

[0064] Example 3: Testing of the Franz Transdermal Diffusion Apparatus

[0065] test group:

[0066] Comparison group: 5 μg / mL 11AA ramdon oligo (amino acid sequence is ACSSSPSKHC), 40 μg / mL polypeptide factor;

[0067] CPEP-9 group: 5μg / mL CPEP-9, 40μg / mL polypeptide factor;

[0068] CPEP-10N group: 5 μg / mL CPEP-10N, 40 μg / mL polypeptide factor;

[0069] CPEP-10C group: 5μg / mL CPEP-10C, 40μg / mL polypeptide factor;

[0070] CPEP-11 group: 5μg / mL CPEP-11, 40μg / mL polypeptide factor;

[0071] CPEP combination group: 5 μg / mL CPEP combination (weight ratio of CPEP-9 / 10N / 10C / 11=1:1:1:2), 40 μg / mL polypeptide factor.

[0072] Test method: take intact and undamaged SD rat (purchased from Beijing Weitong Lihua Experimental Animal Center) abdominal skin tissue, remove hair and place in normal saline (0.9% NaCl aqueous solution) for use. Transdermal tank (such as figure 1 Shown) the lower tank (receptor chamber) was filled with 5ml of normal saline and a magnetic stirrer (stir bar...

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Abstract

The invention relates to a cell infiltration enhancing peptide, a composition comprising the cell infiltration enhancing peptide and combination thereof, peptide factors and optional ATP and application of the composition in cosmetics or skin care products. The invention further relates to a preparation method of the composition. The cell infiltration enhancing peptide and the combination thereofcan effectively enhance a transdermal effect of the peptide factors and other active large molecules, and then beauty and skin care effects of the peptide and the combination thereof can be better achieved.

Description

technical field [0001] The invention belongs to the technical field of cosmetics, and in particular relates to cell penetration enhancing peptides, compositions containing the cell penetration enhancing peptides, and their application in the preparation of cosmetics or skin care products. Background technique [0002] Transdermal delivery of functional ingredients is a compelling systemic drug delivery route that is painless, reliable, targeted, and highly effective. However, transdermal delivery of macromolecules remains a challenge due to the limitation of use by the skin barrier, especially that of the stratum corneum. In the field of cosmetics, biomacromolecular active ingredients such as oligopeptides, polypeptide factors, and active cell extracts are increasingly recognized by the industry due to their remarkable efficacy, but these functional ingredients need to pass through the skin barrier to activate skin cells. , so the development of a transdermal solution for a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/06A61K8/64A61Q19/00
CPCA61K8/64A61Q19/00C07K7/06
Inventor 李钧翔周碧柳陆益
Owner HARVEST BIOTECH CO LTD
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