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Immunochromatographic detection card for rapidly detecting chitosanase 3-like protein 1 and preparation method thereof

A chitinase and immunochromatography technology, which is applied in measurement devices, analytical materials, instruments, etc., can solve the problem of no chitinase activity, achieve excellent accuracy and precision, wide linear range, and improve detection efficiency. Effect

Pending Publication Date: 2019-11-22
TIANJIN SAVANT BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can bind chitin, but has no chitinase activity

Method used

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  • Immunochromatographic detection card for rapidly detecting chitosanase 3-like protein 1 and preparation method thereof
  • Immunochromatographic detection card for rapidly detecting chitosanase 3-like protein 1 and preparation method thereof
  • Immunochromatographic detection card for rapidly detecting chitosanase 3-like protein 1 and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] 1. Preparation of CHI3L1 immunochromatographic test card

[0044] 1) Preparation of surface activated fluorescent latex microspheres:

[0045] N,N'-bislauroyl ethylenediamine diacrylate sodium, polyethylene glycol monolauric acid ester, dodecylbenzene sulfonate and lauroyl glutamate, the weight ratio of the three is 0.5:4 :0.8:5.

[0046] ①Take the surfactant (containing 0.5mg of N,N'-bislauroyl ethylenediamine diacrylate sodium, 4mg polyethylene glycol monolauric acid ester, 0.8mg dodecylbenzene sulfonate and 5mg Lauroyl glutamate) was added to 0.2mol / L, pH=8.4 boric acid-borax buffer solution (containing 0.5wt% ~ 3wt% PEG2000), and then 1mL dimethylformamide, 1mg N, N '-Dicyclohexylcarbodiimide and 0.5mg N-hydroxysuccinimide, react at a stirring speed of 120r / min;

[0047] ②Take 1mL of 1wt% fluorescent latex microsphere dispersion with carboxyl groups on the surface (purchased from Shanghai Zhenzhun Biotechnology Co., Ltd.), and use 10mL, 0.2mol / L, pH=8.4 boric acid-borax b...

Embodiment 2

[0086] 1. Preparation of CHI3L1 immunochromatographic test card

[0087] 1) Preparation of surface activated fluorescent latex microspheres:

[0088] N,N'-bislauroyl ethylenediamine sodium diacrylate, polyethylene glycol monolauric acid ester, dodecylbenzene sulfonate and lauroyl glutamate, the weight ratio of the three is 3:5 :2:6.

[0089] ①Take the surfactant (containing 3mg of N,N'-bislauroyl ethylenediamine diacrylate sodium, 5mg of polyethylene glycol monolauric acid ester, 2mg of dodecylbenzene sulfonate and 6mg of laurel Acylglutamate) was added to 0.2mol / L, pH=8.4 boric acid-borax buffer solution (containing 0.5wt% to 3wt% PEG2000), and then 1mL dimethylformamide, 1mg N,N'- Dicyclohexylcarbodiimide and 0.5mg N-hydroxysuccinimide were reacted at a stirring speed of 120r / min;

[0090] ② Take 1 mL of a dispersion containing 1 wt% of fluorescent latex microspheres with carboxyl groups on the surface (purchased from Shanghai Zhenzhun Biotechnology Co., Ltd.), and use 10 mL, 0.2m...

Embodiment 3

[0119] 1. Preparation of CHI3L1 immunochromatographic test card

[0120] 1) Preparation of surface activated fluorescent latex microspheres:

[0121] N,N'-bislauroyl ethylenediamine diacrylate sodium, polyethylene glycol monolauric acid ester, dodecylbenzene sulfonate and lauroyl glutamate, the weight ratio of the three is 4:7 :2.5:13.

[0122] ①Take the surfactant (containing 4mg of N,N'-bislauroyl ethylenediamine diacrylate sodium, 7mg polyethylene glycol monolauric acid ester, 2.5mg dodecylbenzene sulfonate and 13mg Lauroyl glutamate) was added to 0.2mol / L, pH=8.4 boric acid-borax buffer solution (containing 0.5wt% ~ 3wt% PEG2000), and then 1mL dimethylformamide, 1mg N, N' -Dicyclohexylcarbodiimide and 0.5mg N-hydroxysuccinimide, react at a stirring speed of 120r / min;

[0123] ②Take 1mL of 1wt% fluorescent latex microsphere dispersion with carboxyl groups on the surface (purchased from Shanghai Zhenzhun Biotechnology Co., Ltd.), and use 10mL, 0.2mol / L, pH=8.4 boric acid-borax buf...

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Abstract

The invention relates to an immunochromatographic detection card for rapidly detecting chitosanase 3-like protein 1. The immunochromatographic detection card comprises a test strip, wherein the test strip comprises a detection line and a quality control line, the detection line is coated with mouse-anti-human CHI3L1 monoclonal antibodies, and the quality control line is coated with goat-anti-rabbit polyclonal antibodies. The immunochromatographic detection card for rapidly detecting chitosanase 3-like protein 1 provided by the invention can be used for detecting serum, plasma and whole blood,so as to be applied to diagnosis of liver fibrosis, liver cirrhosis and progress rates thereof.

Description

Technical field [0001] The invention belongs to the field of in vitro diagnosis, and relates to an immunochromatographic detection card for rapidly detecting chitinase 3-like protein 1 and a preparation method thereof. Background technique [0002] Chitinase protein, full name: Chitinase 3-like protein 1 (Chitinase 3-like protein 1, CHI3L1) is a kind of 383 amino acids, it is often called YKL-40, and the three N-terminal amino acids ( Tyr-Lys-Leu-Leu, YKL) and its molecular weight (40Kda). It is a member of glycosyl hydrolase family. It can bind chitin, but it has no chitinase activity. [0003] In recent years, a large number of studies have shown that YKL-40 is a powerful fibrosis marker with high diagnostic accuracy, especially in hcv-related liver diseases. Its determination can confirm and improve the diagnostic accuracy, especially in the early stage of liver fibrosis; and YKL-40 serum markers can predict advanced fibrosis and liver cirrhosis. It increases with the degree...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/533G01N33/543
CPCG01N33/558G01N33/533G01N33/54313
Inventor 林斯石岗胡文翔
Owner TIANJIN SAVANT BIOTECH CO LTD
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