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Construction method of miR155 overexpression mouse tongue squamous cell cancer model

A tongue squamous cell carcinoma, construction method technology, applied in the field of construction of miR155 overexpression mouse tongue squamous cell carcinoma model, can solve the problems of human TSCC etiology and pathogenesis gap, individual differences, long induction time and other problems, Achieve good promotion and application value, stable characterization, and good repeatability

Inactive Publication Date: 2019-11-22
STOMATOLOGY AFFILIATED STOMATOLOGY HOSPITAL OF GUANGZHOU MEDICAL UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above chemical induction methods have a long induction time, low modeling efficiency, and large individual differences, which are still far from the etiology and pathogenesis of human TSCC.

Method used

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  • Construction method of miR155 overexpression mouse tongue squamous cell cancer model
  • Construction method of miR155 overexpression mouse tongue squamous cell cancer model
  • Construction method of miR155 overexpression mouse tongue squamous cell cancer model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Construction of tongue squamous cell carcinoma model in mice with miR155 overexpression

[0037] A method for constructing a miR155 overexpression mouse tongue squamous cell carcinoma model, comprising the following steps:

[0038] (1) Verification of miR155 transgenic mice and FVB mice

[0039] The miR155 transgenic mice were mass-produced in the form of mREF positive × mREF positive or mREF positive × mREF negative, and the postnatal transgenic mice were detected with a small animal imager for REF signals to identify miR155 transgenic mice (positive REF signals) or FVB Mice (litmate control, negative REF signal); detection of Luc signal distribution in the whole body of miR155 transgenic mice to confirm the overexpression of miR-155 gene in the tongue of miR155 transgenic mice.

[0040](2) 4NQO induction treatment

[0041] 1) Preparation of reagents

[0042] Preparation of reagent A: first dissolve 4-nitroquinoline-1-oxide (4-nitroquinoline-oxide, 4NQO) i...

Embodiment 2

[0054] Example 2 General Morphological Observation of miR155 Transgenic Mice

[0055] 1. Experimental method

[0056] During the process of raising mice in step (3) in Example 1, observe the mental state, exercise status, diet, water intake, body weight, coat color and excretion of the mice every day, and record the water intake and body weight of the mice every week , and check the changes in the oral cavity of the mice, record the color and texture of the oral mucosa of the mice including the back of the tongue, the belly of the tongue, and the floor of the mouth, and observe whether there are plaques, ulcers or tumors.

[0057] 2. Experimental results

[0058] The results of the morphological characteristics of the mice in the experimental group and the control group until the 10th week were as follows: figure 1 As shown, it can be seen that when the 4NQO solution was fed to the 10th week, it was found that the mice in the experimental group 1 and the experimental group ...

Embodiment 3

[0061] Example 3 Analysis of TSCC process in miR155 transgenic mice

[0062] 1. Experimental method

[0063] After the experimental group and control group mice constructed in Example 1 were put to death, if the mouse tongue was confirmed to have precancerous lesions or tumors, then measure the size of the tongue lesions, tongue tumor number, weight, length (L), width (W), calculate tumor volume, carry out TSCC process analysis;

[0064] The formula for calculating tumor volume is as follows: V=L×W 2 ×0.5.

[0065] 2. Experimental results

[0066] The scoring criteria for the analysis of TSCC progress in mice are shown in Table 1, which can clearly and intuitively represent the progress of TSCC in mice.

[0067] Table 1 Scoring criteria for mouse TSCC process analysis

[0068]

[0069] The number of tumors in the tongue of mice in the experimental group was as follows: Figure 4 As shown in Figure a, it can be seen that the number of tongue tumors in miR155 transgenic...

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Abstract

The invention discloses a construction method of a miR155 overexpression mouse tongue squamous cell cancer model. According to the method, a miR155 gene is overexpressed first in a mouse body, and then a mouse is fed with a 4NQO solution for more than 10 weeks to obtain the tongue squamous cell cancer model. Compared with an existing TSCC animal model, the model has stable representation and can successfully induce the precancerous lesion of the mouse in a short time, truly simulate the dynamic change process of the occurrence and development of a tongue squamous cell cancer tumor and serve asan ideal model for researching the molecular mechanism of the occurrence and development of tongue squamous cell cancer, screening of molecular targets for diagnosis and treatment and exploring of anew treatment method. In addition, the construction method of the model is simple and feasible, the tumor-forming latent period is short, the modeling accuracy is high, the repeatability is high, themodeling success rate reaches 95.0% or above, and the model has a great application and popularization value in screening medicaments for treating tongue squamous cell cancer.

Description

technical field [0001] The invention belongs to the technical field of animal model construction. More specifically, it relates to a method for constructing a miR155-overexpressed mouse tongue squamous cell carcinoma model. Background technique [0002] Tongue squamous cell carcinoma (tongue squamous cell carcinoma, TSCC) is the most common oral malignancy, accounting for 90% of oral malignancies. Although many important advances have been made in cancer treatment, the mortality rate of TSCC has remained basically unchanged since the 1960s. The 5-year survival rate in the mid-term is about 50%, and the 5-year survival rate in the late stage is less than 20%. Of all measures to reduce TSCC mortality and disability, early diagnosis and prevention are the best. The etiology of TSCC shows that a single factor is difficult to cause TSCC, and multiple factors such as genetics, chemical factors, physical factors, and biological carcinogenic factors combine to cause the occurrence...

Claims

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Application Information

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IPC IPC(8): A01K67/027
CPCA01K67/027A01K2227/105A01K2267/0331
Inventor 吴丽红肖东曾明涛林晓琳蔡美娟江颖彤
Owner STOMATOLOGY AFFILIATED STOMATOLOGY HOSPITAL OF GUANGZHOU MEDICAL UNIV
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