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Artificial islet or artificial pancreas and preparation method thereof

An artificial pancreas and pancreatic islet technology, applied in the field of artificial pancreatic islets, can solve the problems that have not been reported in the field of artificial organs, and achieve the effects of good biodegradability and biocompatibility, mild reaction conditions, and simple and easy operation.

Active Publication Date: 2019-11-19
INST OF BIOMEDICAL ENG CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, compared with the traditional view that porous balls are loaded with non-selective loading of various cells as injectable cell delivery carriers, using porous balls to approximate the pancreas "net pocket" structure to specifically load islet cells to construct artificial islets or artificial pancreas, making it It has the function of secreting insulin, which has not been reported in the field of artificial organs

Method used

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  • Artificial islet or artificial pancreas and preparation method thereof
  • Artificial islet or artificial pancreas and preparation method thereof
  • Artificial islet or artificial pancreas and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1: prepare PLGA porous microsphere support body:

[0038] S1, fully dissolve 200 mg of PLGA (molecular weight is 10000-100000) in 8 ml of dichloromethane to prepare an oil phase, add 1% NH dropwise under stirring 4 HCO 3 solution (porogen) to form colostrum.

[0039] S2. Transfer the colostrum into 0.1% PVA aqueous solution to form double emulsion and stir for 3 hours; collect PLGA porous microspheres.

[0040] S3. Wash with deionized water for 3 times, use 0.1M NaOH solution for 20 minutes, wash with deionized water for 3 times, and pack.

[0041] S4, obtain PLGA porous microsphere solid powder after freeze-drying.

[0042] The SEM picture of gained PLGA porous microsphere is as follows Figure 4 shown. The average particle size range of the obtained PLGA porous microsphere is 50-500 μm, and the diameter of the pores in the porous microsphere structure is 5-50 μm.

Embodiment 2

[0043] Example 2: Planting beta-TC-6 cells on PLGA porous microspheres

[0044]Let stand the PLGA microsphere suspension obtained in Example 1, remove the suspension, and set aside; count the beta-TC-6 cells after trypsinization, take about 1×10^7 cells and place them in a 20mL syringe, and dilute to 4mL , pumped 40-60 times under negative pressure, and placed in the medium with PLGA porous balls and cultured for 7 days. Take the co-culture system of PLGA microspheres and beta-TC-6 cells, fix them, make embedded sections, stain the frozen sections, and analyze the morphology and survival of cells in the PLGA porous spheres, such as Figure 5 with Image 6 shown. It was found by frozen section that there were insulin-secreting cells in the porous spheres and the cells were in good shape; the overall shape and size were similar to normal mouse islets.

Embodiment 3

[0046] The insulin release test was carried out on the co-cultured cells on day 7: that is, 20 mmol / L high-concentration glucose solution was added to the cells and / or PLGA porous microspheres, and the insulin content in the system was detected.

[0047] Embodiments of the present invention have also analyzed the situation that co-cultured cells secrete insulin under low-concentration glucose solution (2-5mmol / L), such as Figure 7 shown. Studies have found that porous sphere-based artificial islets or artificial pancreas have the function of secreting insulin after high glucose stimulation.

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Abstract

The invention discloses an artificial islet or artificial pancreas and a preparation method thereof. The artificial islet or artificial pancreas includes a supporting body with a porous structure andcells capable of secreting insulin and growing in the porous structure of the supporting body. The preparation method of the artificial islet or artificial pancreas comprises the steps that a PLGA porous sphere supporting body is prepared; the cells capable of secreting the insulin are planted in the PLGA porous sphere supporting body, and the cells planted in the PLGA porous sphere supporting body are detected. The construction of the artificial islet or artificial pancreas is based on the porous structure of PLGA or a polylactic acid polymer sphere and the good biodegradability and biocompatibility thereof, and effectively simulating the structure and physiological function of an islet is facilitated. The insulin secretory cells are set in the constructed artificial islet or artificial pancreas porous sphere, the cell morphology is good, the overall morphology and size are similar to normal mouse islets, and an effective solution is provided for solving the shortage of donors in clinical islet transplantation.

Description

technical field [0001] The invention belongs to the technical field of artificial pancreas, and in particular relates to an artificial pancreas or artificial pancreas and a preparation method thereof. Background technique [0002] At present, the number of diabetic patients in the world is about 425 million, and more than 4 million people die from diabetes every year. Diabetes mellitus is a metabolic disease caused by relative or absolute deficiency of insulin secretion. Therefore, insulin is the drug of choice for the treatment of diabetes. However, the current treatment of diabetes mainly relies on exogenous insulin injections, which cannot accurately simulate the changing insulin requirements in the body. Therefore, insulin injection still has deficiencies in controlling fasting blood sugar and treating diabetes and its complications. Most hypoglycemic drugs rely on stimulating islet cells to release insulin or enhancing the sensitivity of metabolic organs to insulin, a...

Claims

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Application Information

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IPC IPC(8): A61L27/38A61L27/18A61L27/50A61L27/56A61L27/58
CPCA61L27/18A61L27/3834A61L27/50A61L27/56A61L27/58C08L67/04
Inventor 李宸周志敏刘默涵杨雨舟
Owner INST OF BIOMEDICAL ENG CHINESE ACAD OF MEDICAL SCI
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