Cell preservation liquid capable of realizing long term effective preservation of cells

Abstract

The invention discloses a cell preservation liquid capable of realizing long term effective preservation of cells. The cell preservation liquid capable of realizing long term effective preservation ofcells comprises following components, disodium hydrogen phosphate 0.1-3g, potassium dihydrogen phosphate 0.1-4.1g, potassium chloride 0.01-2.2g, sodium chloride 0.6-1g, calcium chloride 0.001-0 0.01g, glacial acetic acid 0.1-7g, ethylene diamine tetraacetic acid 1-16g, trihytdroxy methyl-aminomethane 0.001-0.003g, sucrose 0.1-0.24g, and a surfactant 0.98-2.6g; the cell preservation liquid is diluted to 100mL with deionized water, and pH value is controlled to be 6.8 to 7.6. The beneficial effects are that: the cell preservation liquid is capable of maintaining cell activity in a relatively long term, so that long time effective preservation of cells is realized; when the cell preservation liquid is used in preservation of cells, in long preservation term, the cell preservation liquid is still capable of inhibiting breeding of microorganisms, and protecting cells from damage by microorganisms.

Description

technical field [0001] The invention relates to a cell preservation solution, in particular to a cell preservation solution capable of effectively preserving cells for a long time, and belongs to the field of medical biotechnology. Background technique [0002] Cell is the most basic unit of life and has application value in many aspects. For cells and cell products, the difference from ordinary biological products and pharmaceuticals is that cell activity needs to be maintained, and maintaining cell activity is a great challenge. [0003] Physiological saline is safe and convenient in clinical application, but the activity of cells decreases very quickly in saline, and the activity of cells loses as much as 30% after being stored in saline for 10 hours. It can be seen that normal saline is only suitable for short-term storage of cells. [0004] At present, there are some cell preservation solutions on the market, but the storage time of cells in these preservation solutio...

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Problems solved by technology

[0004] At present, there are some cell preservation solutions on the market, but the storage time of cells in these preservation solutions is generally within 30 days. Although the storage time is longer than that in normal saline, there is still a big gap with the actual demand.

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