Detection method of arginine isomers

A detection method and arginine technology, applied in the field of biomedicine, can solve the problems of D-arginine affecting the quality of L-arginine products, and achieve the effects of improving sensitivity, improving separation and ensuring stability

Active Publication Date: 2019-11-12
JING JING PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Aiming at the problems that the content of D-arginine in the existing L-arginine production process is difficult to be sensitively and accurately measured and affects the quality of L-arginine products, the invention provides a detection method for arginine isomers method

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  • Detection method of arginine isomers

Examples

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Embodiment 1

[0023] A method for detecting arginine isomers, comprising the steps of:

[0024] (1) Accurately weigh 80mg of o-phthalaldehyde and 93mg of N-acetyl-L-cysteine, put them in the same 25ml volumetric flask, add 2.5ml of ethanol to dissolve, then add boric acid buffer solution with a pH of 9.8 to dilute to the mark , constant volume, obtain derivatization reagent;

[0025] (2) Accurately weigh an appropriate amount of D-arginine reference substance, and prepare an aqueous solution of the reference substance with a concentration of 1 μg / ml. Precisely measure 2ml of the above-mentioned reference substance aqueous solution and 0.6ml of the derivatization reagent and place them in the same 5ml volumetric flask, add boric acid buffer solution to dilute to the mark, shake well, and constant volume to obtain the reference substance solution;

[0026] (3) Accurately weigh 10 mg of the product to be tested, place it in a 10 ml volumetric flask, add water to dissolve and dilute to the mar...

Embodiment 2

[0034] A method for detecting arginine isomers, comprising the steps of:

[0035] (1) Accurately weigh 80mg of o-phthalaldehyde and 93mg of N-acetyl-L-cysteine, put them in the same 25ml volumetric flask, add 2.5ml of ethanol to dissolve, then add boric acid buffer solution with pH 8 to dilute to the mark , constant volume, obtain derivatization reagent;

[0036] (2) Accurately weigh an appropriate amount of D-arginine reference substance, and prepare an aqueous solution of the reference substance with a concentration of 1 μg / ml. Precisely measure 2ml of the above-mentioned reference substance aqueous solution and 0.5ml of the derivatization reagent and place them in the same 5ml volumetric flask, add boric acid buffer solution to dilute to the mark, shake well, and constant volume to obtain the reference substance solution;

[0037] (3) Accurately weigh 10 mg of the product to be tested, place it in a 10 ml volumetric flask, add water to dissolve and dilute to the mark, shak...

Embodiment 3

[0043] A method for detecting arginine isomers, comprising the steps of:

[0044] (1) Accurately weigh 80mg of o-phthalaldehyde and 93mg of N-acetyl-L-cysteine, put them in the same 25ml volumetric flask, add 2.5ml of ethanol to dissolve, then add boric acid buffer solution with pH 10 to dilute to the mark , constant volume, obtain derivatization reagent;

[0045] (2) Accurately weigh an appropriate amount of D-arginine reference substance, and prepare an aqueous solution of the reference substance with a concentration of 1 μg / ml. Precisely measure 2ml of the above-mentioned reference substance aqueous solution and 0.75ml of the derivatization reagent and place them in the same 5ml volumetric flask, add boric acid buffer solution to dilute to the mark, shake well, and constant volume to obtain the reference substance solution;

[0046](3) Accurately weigh 10 mg of the product to be tested, place it in a 10 ml volumetric flask, add water to dissolve and dilute to the mark, sha...

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Abstract

The invention relates to the field of biomedical technology, and specifically discloses a detection method of arginine isomers. The detection method comprises the steps of: taking a D-arginine reference substance to prepare a reference substance aqueous-solution, and preparing a reference substance solution from the reference substance aqueous-solution and a derivative reagent; taking a to-be-detected substance to prepare a to-be-detected substance aqueous-solution, and preparing a to-be-detected substance solution from the to-be-detected substance aqueous-solution and a derivative reagent; and respectively taking the reference substance solution and the to-be-detected substance solution, using liquid chromatography for detection, and using an external standard method to calculate the content of D-arginine in the to-be-detected substance. According to the detection method of the arginine isomers provided by the invention, specificity is high, sensitivity is high, accuracy is high, theD-arginine content in L-arginine can be effectively controlled, and L-arginine product quality is guaranteed.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a method for detecting arginine isomers. Background technique [0002] There are L-type and D-type of arginine, the naturally occurring one is L-arginine, the production method of L-arginine is mainly microbial fermentation method, and the production of D-arginine is unavoidable in the production process of. In order to improve and ensure the quality of L-arginine products, the content of D-arginine must be strictly controlled. [0003] At present, there are few reports on the detection method of the content of isomers in arginine, and arginine is an α-amino acid, and its terminal absorption is at ultraviolet wavelengths, which leads to inability to be sensitive in the production process of L-arginine. , Accurate control of D-arginine content. Contents of the invention [0004] Aiming at the problems that the content of D-arginine in the existing L-arginine production pr...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/74
CPCG01N30/02G01N30/06G01N30/74
Inventor 刘超李斌水李晓微汪飞石江涛梁云科刘翠环李素霞李海松张亮武存景
Owner JING JING PHARMA
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