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Method for identifying Gnaphalium L. plant varieties through ITS2 (internal transcribed spacer 2)

A technology for the genus and plant species of Aspergillus, which is applied in biochemical equipment and methods, sequence analysis, and microbial determination/inspection, etc., can solve problems such as low accuracy, and achieve the effect of improving accuracy

Active Publication Date: 2019-11-12
XIAN MEDICAL UNIV
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Problems solved by technology

[0005] The purpose of the present invention is to provide a method for identifying plant species of the genus Sagebrush using the ITS2 sequence, which solves the problem of low accuracy of the existing Sagebrush identification methods

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  • Method for identifying Gnaphalium L. plant varieties through ITS2 (internal transcribed spacer 2)
  • Method for identifying Gnaphalium L. plant varieties through ITS2 (internal transcribed spacer 2)
  • Method for identifying Gnaphalium L. plant varieties through ITS2 (internal transcribed spacer 2)

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Embodiment Construction

[0032] The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0033] A kind of method of utilizing ITS2 sequence of the present invention to discriminate the plant species of the genus Salmonella, comprises the following steps:

[0034] Step 1, take a number of leaves of the plant samples to be identified, and extract the DNA of the leaves of the plant samples to be identified, as shown in Table 1, wherein the source of the samples can be 8 species of S. 1 species of S. chinensis (serial number 35 in Table 1) and 1 species of edelweiss (serial number 38 in Table 1), which are selected plant samples for identification;

[0035] Table 1 Sources of plant samples to be identified

[0036]

[0037]

[0038] Step 1.1, take several leaves of the above-mentioned plant samples to be identified, put them into centrifuge tubes with a volume of 1.5mL, and place them in liquid nitrogen for quick freezing, the...

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Abstract

The invention discloses a method for identifying Gnaphalium L. plant varieties through an ITS2 (internal transcribed spacer 2). The method includes the steps that 1, DNA of leaves of a to-be-identified plant sample is extracted; 2, the DNA of the leaves of the to-be-identified plant sample is subjected to PCR amplification of the nucleotide sequence, namely the ITS2; 3, a PCR product is subjectedto bidirectional sequencing, so as to obtain the ITS2 of the leaves of the to-be-identified plant sample; 4, the rDNA ITS2 of a homologous sequence and an external variety sequence is obtained; and 5,the K2P genetic distance is calculated, a phylogenetic tree is constructed, and the Gnaphalium L. plant varieties are identified. According to the method for identifying the Gnaphalium L. plant varieties through the ITS2, the molecular identification basis is provided for identification of cudweed herb medicines, cudweed herb and articles prone to being mixed up with the cudweed herb can be accurately and rapidly detected from various Chinese herbal medicines, detection accuracy is improved, and the method is suitable for wide popularization and application.

Description

technical field [0001] The invention belongs to the technical field of biological identification methods, and in particular relates to a method for identifying plant varieties of the genus Salmonella by using an ITS2 sequence. Background technique [0002] Gnaphalium L. belongs to Angiosperms, Dicotyledons, Compositae, mostly annual herbs, a few are perennial herbs, stems are herbaceous or slightly woody at the base, densely covered with white cotton wool or fluff ;The leaves are alternate leaves, entire, without or with short stalks; the inflorescences are capitulums, cymose or spreading cone-shaped, the involucre is ovate or bell-shaped, and the involucre is mostly golden yellow or light yellow Or yellowish brown, a few are reddish brown, with 2-4 or 5 layers, the back is cotton-haired, the corolla is yellow or light yellow; achene. [0003] It is recorded in "Flora of China" that there are nearly 200 species of plants of the genus Salmonella in the world, and 19 species ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6869C12Q1/689G16B30/10
CPCC12Q1/6869C12Q1/689G16B30/10C12Q2531/113Y02A50/30
Inventor 郑梦迪张寒张彦
Owner XIAN MEDICAL UNIV
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