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Evaluation method of teleostean alkaline resistance

An evaluation method and technology for bony fish, applied in the direction of color/spectral characteristic measurement, instruments, measuring devices, etc., can solve the indicators that cannot be used for alkali-tolerant breeding of fish, cannot judge the adaptation process of fish, and cannot be domesticated in saline-alkali water. Provide reference and other issues to achieve the effect of short identification cycle

Active Publication Date: 2019-11-08
EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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Problems solved by technology

The problems with the semi-lethal method are: firstly, the alkalinity concentration used in the semi-lethal method is usually higher, which is quite different from the natural real alkalinity concentration, and cannot be directly used to evaluate the tolerance of fish to the natural alkalinity concentration; secondly, the lethality The test cannot judge the adaptation process of fish to the high-alkaline environment, and cannot provide a reference for domestication technology in saline-alkaline water; third, the physiological functions of fish undergo substantial changes under lethal concentrations, and cannot be directly used as a guideline for alkali-resistant breeding of fish. Index, the present invention has overcome above-mentioned technical problem

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  • Evaluation method of teleostean alkaline resistance
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Embodiment 1

[0034] 1. Test material:

[0035] The naked carp juveniles for the test were provided by the Qinghai Lake Naked Carp Rescue Center. Before the test started, they were temporarily raised indoors for more than one month. During the temporary rearing period, they were fed twice a day with a full amount of 5% of their body weight, and the water was changed every day. , uninterrupted inflation. Temporary breeding water is filtered tap water (Kaneng AC / KDF150-1-300), pH (7.63±0.05), temperature (17.0±0.5)°C, dissolved oxygen (9.14±0.03) mg / L, ammonia nitrogen (0.01±0.01) mmol / L. The test selected healthy juvenile fish of the same size, body weight (1.39±0.09) g, and body length (5.12±0.07) cm, and the feeding was stopped 48 hours before the test.

[0036] 2. Experimental water preparation:

[0037]According to the life history of naked carps in Qinghai Lake (sexually mature naked carps travel back to freshwater channels from Qinghai Lake from April to July every year, and after s...

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Abstract

The invention relates to an evaluation method of teleostean alkaline resistance. The alkaline tolerance of the fish is determined through the influence on the fish plasma nitrogen waste concentrationtesting by carbonate alkalinity stress. The evaluation method disclosed by the invention is short in experiment identification cycle and free from the limitation of the external water quality condition, the evaluation is free from expending a large amount of experiment expense, and is non-lethal; the evaluation method is comparatively economic, convenient and fast and non-lethal alkaline resistance identification method.

Description

technical field [0001] The invention belongs to the field of alkalinity tolerance evaluation, in particular to an evaluation method for the alkalinity resistance of bony fishes. Background technique [0002] Changes in pH and alkalinity are key factors in stressing fish during aquaculture and in some natural water environments. There are 690 million mu of saline-alkali water resources in my country, covering 19 provinces, municipalities and autonomous regions in China, most of which belong to non-ocean saline water resources. state. my country took the lead in carrying out saline-alkali aquaculture in the coastal areas of North China and the areas along the Yellow River. In Hebei, Shandong, Jiangsu, Tianjin, Gansu, Ningxia, Shaanxi, Shanxi, Jilin, Henan, Xinjiang and other provinces and autonomous regions, there are different levels of saline-alkaline water The development and utilization of resource fishery has created a new way for the development and utilization of saline...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50G01N21/31
CPCG01N33/5091G01N21/31Y02A40/81
Inventor 么宗利来琦芳周凯高鹏程衣晓飞刘一萌孙真
Owner EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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