A kind of identification method of cotton alkali resistance

A technology of cotton and alkali resistance, which is applied in the fields of seed and rhizome treatment, agriculture, application, etc., can solve the problems of less research on cotton alkali resistance, and achieve the effect of short test period

Active Publication Date: 2020-04-28
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0003] Cotton is a pioneer crop in saline-alkali land. Previous studies on cotton's salt tolerance have been in-depth, and there are few studies on cotton's alkali resistance.

Method used

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  • A kind of identification method of cotton alkali resistance
  • A kind of identification method of cotton alkali resistance
  • A kind of identification method of cotton alkali resistance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0024] (1) Seed pretreatment:

[0025] Cotton seeds are develveted with concentrated sulfuric acid;

[0026] Select seeds with consistent maturity, soak them in 20%-30% hydrogen peroxide for 3-4 hours, wash them with sterile water 5 times (1 min each time) and carry out disinfection treatment;

[0027] Seeds were soaked in sterile water for 12 hours to accelerate germination.

[0028] (2) Alkaline stress condition screening

[0029] Material: conventional cotton varieties (GK50, Yu 2067, Ji 169)

[0030] Select 100 seeds of the same size and "white" (3 repetitions);

[0031] Vertical double-layer filter paper method: spread the filter paper on a plastic plate, and use (10, 25, 40mM) Na 2 CO 3 Wet the filter paper, and use clear water as a blank control. Place the seed growth points on the filter paper in the same direction, with a horizontal interval of 6 cm and a vertical interval of 5 cm between the seeds. Roll up the filter paper horizontally along the seed position, a...

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Abstract

The invention provides a method for identifying the alkali resistance of cotton germplasm, which comprises: cotton seed delint treatment; sequentially soaking in hydrogen peroxide and rinsing with sterile water; soaking in sterile water to accelerate germination; (2) carrying out alkali stress on "Lubai" seeds treatment, clear water was used as a contrast, and the radicle survival rate under the two treatments was investigated, and a comprehensive evaluation of alkali resistance was carried out; the relative survival rate of the radicle root was calculated with Zhong 9807 as an identification reference material, and the alkali resistance evaluation standard of cotton germplasm was obtained; (3) The cotton germplasm to be identified was pretreated and treated with alkali stress, the survival rate of the radicle was investigated, the relative survival rate of the radicle was calculated, and the alkali resistance level of the cotton germplasm was identified according to the cotton germplasm alkali resistance evaluation standard. The condition of alkali stress treatment is: 25mM Na 2 CO 3 Treated for 5 days under alkaline conditions. The present invention uses the stress germination method to carry out indoor alkali resistance identification of cotton germplasm, and the cycle is 5 days, the test cycle is short, not limited by external climate conditions, and does not need to spend a lot of test costs, and establishes an economical, convenient and fast method. Cotton alkali resistance identification method.

Description

technical field [0001] The invention belongs to the variety evaluation technology in the field of agriculture, and in particular relates to an alkali-resistant identification method in the germination stage of cotton, which is suitable for screening and evaluation of cotton alkali-resistant germplasm resources. Background technique [0002] Soil salinization has always been an important factor affecting plant growth and development and reducing crop yield. According to incomplete statistics from the United Nations Educational, Scientific and Cultural Organization (UNESCO) and the Food and Agriculture Organization (FAO), the area of ​​saline-alkali land in the world is about 954 million hm 2 (ZhangT.L, 2000), with unreasonable crop rotation, and blind or excessive application of chemical fertilizers, the secondary salinization of soil has been exacerbated. Studies have shown that when the soluble salt content of the soil exceeds 0.3%, it will cause soil salinization. Saline...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01C1/00A01C1/02
CPCA01C1/00A01C1/025
Inventor 叶武威张冰蕾舒娜王俊娟王德龙陆许可陈修贵韩明格王帅崔瑞峰樊伟莉郭丽雪陈超
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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