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Constructing method of AFFT1 cells

A cell and antigen epitope technology, applied in the field of AFFT1 cells and its preparation, can solve problems such as T cell self-protection technology, and achieve the effect of improving lethality

Active Publication Date: 2019-11-05
BEIJING DCTY BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0005] None of the above schemes consider the self-protection technology of T cells, so that a small number of specific T cells directly face the powerful immune microenvironment

Method used

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  • Constructing method of AFFT1 cells
  • Constructing method of AFFT1 cells
  • Constructing method of AFFT1 cells

Examples

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Embodiment Construction

[0101] The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention

[0102] 1. Whole Exome Sequencing

[0103] 1) Take peripheral blood from lung cancer patients for ctDNA sequencing and HLA typing;

[0104] 2) Use software to analyze the sequencing information: compare the ctDNA sequencing results with the genome of normal cells, and screen out the mutation sites;

[0105] 2. Epitope prediction

[0106] 1) Take the mutated amino acid site as the center, extend 10 amino acids to both sides, and use this 21-amino acid polypeptide as a "potential antigenic epitope";

[0107] 2) Use prediction software to analyze the IC50 of the potential antigenic epitope (recommended software: NetMHCpan 3.0, PickPocket, artificial neural networks (ANN)), if IC50<1000nM, the potential antigenic epitope is considered as "antigenic epitope";

[0108] 3. Synthetic peptides

[0109] Antigen epitope peptide synthesis method...

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Abstract

The invention relates to a preparation method of AFFT1 cells. RFF cells are transformed through a TCR-T technical principle, then immunosuppression target spots of transformed T cells are knocked outthrough a gene editing technology, thus the specific cytotoxic T cells are precisely protected against being inhibited in vivo, and the lethality of the T cells on tumor cells is improved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an AFFT1 cell and a preparation method thereof. Background technique [0002] At present, in terms of tumor-specific immunotherapy, the existing LAK, DC, CIK, and DC-CIK cells and methods have basically been proven to be ineffective, while NK, CAR-NK, TIL, and other cell technologies have yet to mature. CAR - T cells have shortcomings in safety and solid tumor therapy. [0003] In the prior art, DCs are generally modified to present T cells to produce specific killing. Some laboratories are trying to use viruses as vectors to transfect and present T cells to induce specific killing of T cells. We have also used mutant hybrid peptides to directly stimulate PBMCs to induce T cells. There are also laboratories using TCR-T technology to target the presentation of MAGE A3 antigen. [0004] The above treatment methods are immature, especially the in vitro induction of DC ce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/90C12N5/10
CPCC07K14/7051C12N5/0638C12N15/907C12N2510/00C12N2800/80C12N2810/10
Inventor 焦顺昌张嵘周子珊解佳森陈小彬陈红利
Owner BEIJING DCTY BIOTECH CO LTD
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