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Application of inhibitor of LncRNA XLOC_110286 in preparation of medicine for promoting angiogenesis

An angiogenesis and inhibitor technology, applied in the fields of cell engineering and genetic engineering

Active Publication Date: 2019-11-05
GUANGDONG LAB ANIMALS MONITORING INST +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In recent years, many literatures have reported that lncRNA affects angiogenesis by regulating human umbilical vein endothelial cells (HUVECs) in tumor diseases, but angiogenesis after MI is rarely reported.

Method used

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  • Application of inhibitor of LncRNA XLOC_110286 in preparation of medicine for promoting angiogenesis
  • Application of inhibitor of LncRNA XLOC_110286 in preparation of medicine for promoting angiogenesis
  • Application of inhibitor of LncRNA XLOC_110286 in preparation of medicine for promoting angiogenesis

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Embodiment 1

[0029] 1. Construction of gene overexpression vector

[0030]The lncRNA XLOC_110286 related to myocardial infarction was screened from the differential lncRNA as the research object. DNAMAN software analysis found that the full-length sequence of the lncRNA XLOC_110286 gene has no Kpn I and Nhe I restriction endonuclease sites, but the pcDNA3.1(-) vector (purchased from BioMed, Cat. No. CL408-01) has Kpn I and NheI restriction sites. The primer premier 5.0 software designed the full-length primers of the gene, and the Kpn I and Nhe I restriction site sequences were added to the upstream and downstream primers respectively. Using human umbilical vein endothelial cell cDNA as a template, PCR amplifies the target fragment (the amplified target fragment is the lncRNAXLOC_110286 sequence, and its nucleotide sequence is shown in SEQ ID NO.1), purified and recovered, double enzyme digested, and connected to pcDNA3 .1 (-) carrier, transformation, screening, and sequencing identifica...

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Abstract

The invention discloses application of an inhibitor of LncRNA XLOC_110286 in preparation of medicine for promoting angiogenesis. It is confirmed that the LncRNA XLOC_110286 is involved in inhabitationof proliferation of human umbilical vein endothelial cells through overexpression and silence of the LncRNA XLOC_110286, apoptosis of the human umbilical vein endothelial cells are promoted, migration of the human umbilical vein endothelial cells is inhibited, formation of human umbilical vein endothelial cell canaliculi is inhabited, and it is further stated that the LncRNA XLOC_110286 may has the inhibiting effect on angiogenesis after infarct. Thus, the inhibitor of the LncRNA XLOC_110286 can be used for preparation of the medicines for promoting angiogenesis. Specifically, the inhibitor of the LncRNA XLOC_110286 can be used for preparation of medicine for promoting angiogenesis after myocardial infarction.

Description

technical field [0001] The invention belongs to the technical field of cell engineering and genetic engineering, and specifically relates to the application of an inhibitor of long-chain non-coding RNA XLOC_110286 (lncRNA XLOC_110286) in the preparation of drugs for promoting angiogenesis. Background technique [0002] Myocardial infarction (MI) is a cardiovascular disease that seriously endangers human health. The disease is based on coronary atherosclerotic stenosis, and the blood clot formed blocks the coronary lumen. Due to long-term ischemia, it eventually leads to myocardial necrosis. Known as "the number one killer of human health". [0003] Angiogenesis refers to the development of new blood vessels from existing capillaries or post-capillary veins, mainly through budding to form fully functional blood vessels, mainly including proliferation, budding and migration of endothelial cells. In the damaged heart, effective vascular remodeling can restore the lesion area t...

Claims

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Application Information

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IPC IPC(8): A61K31/7105A61P9/10A61P9/00
CPCA61K31/7105A61P9/10A61P9/00
Inventor 王希龙张豪潘金春袁晓龙龚宝勇唐龙龙高洪彬李加琪谭伟江张哲梁十
Owner GUANGDONG LAB ANIMALS MONITORING INST
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