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Bacterial strain and complex microbial agent for bioremediation of petroleum-contaminated water, and preparation method and application of agent

A compound bacterial agent and bioremediation technology, which is applied in the field of pollution control, can solve the problems of pollution and low efficiency of bioremediation, and achieve the effects of accelerated degradation rate, complete degradation, and strong adaptability

Active Publication Date: 2019-11-01
WUHAN SHUIZHIGUO ENVIRONMENTAL PROTECTION TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to address the increasingly serious environmental pollution of petroleum and petroleum products and the low efficiency of bioremediation methods, and specifically provide a highly efficient petroleum-degrading bacterial strain and two composite bacterial agents to improve the degradation rate of petroleum hydrocarbons. It is used to efficiently remediate the pollution of petroleum and petroleum products; and to increase the removal rate of ammonium nitrogen while synergistically degrading petroleum and petroleum products

Method used

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  • Bacterial strain and complex microbial agent for bioremediation of petroleum-contaminated water, and preparation method and application of agent
  • Bacterial strain and complex microbial agent for bioremediation of petroleum-contaminated water, and preparation method and application of agent
  • Bacterial strain and complex microbial agent for bioremediation of petroleum-contaminated water, and preparation method and application of agent

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0034] Put 5 mL of Pseudomonas putida activation solution with an effective bacterial concentration of 10 billion cfu / g into the low-concentration petroleum-polluted simulated water body (0.5 g crude oil + 1 L natural lake water) at a temperature of 8 °C, and supplement nitrogen and phosphorus nutrients, and leave it at room temperature. Set aside for 2 weeks.

experiment example 1-1

[0036] Put 5 mL of Pseudomonas vulgaris activation solution with an effective bacterial concentration of 10 billion cfu / g into the low-concentration petroleum-contaminated simulated water body (0.5 g crude oil + 1 L natural lake water) at a temperature of 8 °C, and supplement nitrogen and phosphorus nutrients. Set aside for 2 weeks.

experiment example 2-5

[0038] The difference between Experimental Examples 2-5 and Experimental Example 1 is that the temperature of the polluted water body is different. Except for the above differences, other operations are the same as in Example 1.

[0039] The difference between Experimental Example 2-1, 3-1, 4-1, 5-1 and Experimental Example 1-1 is the difference in the temperature of the polluted water body. Except for the above differences, other operations are the same as in Example 1-1.

[0040] Table 1

[0041] Experimental example temperature °C Degradation rate% Experimental example 1 8 24.3±3.2 Experiment 1-1 8 10.1±4.2 Experimental example 2 10 32.9±3.8 Experiment 2-1 10 15.6±3.1 Experimental example 3 15 34.2±3.2 Experimental example 3-1 15 23.9±4.1 Experimental example 4 20 37.6±2.9 Experimental example 4-1 20 32.2±2.7 Experimental example 5 25 40.8±3.2 Experimental example 5-1 25 41.5±3.6

[0042...

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Abstract

The invention discloses a bacterial strain and complex microbial agent for bioremediation of petroleum polluted water, and a preparation method and application of the agent. The invention particularlydiscloses Pseudomonas putida which is used for bioremediation of petroleum and petroleum product pollution. On one hand, the invention discloses a complex microbial agent A. The complex microbial agent takes the Pseudomonas putida as an active core, is supplemented with bacillus pumilus, bacillus subtilis and bacillus licheniformis, greatly increases the petroleum hydrocarbon degradation rate through a synergistic effect, and has the application potential of efficiently repairing petroleum and petroleum product pollution. On the other hand, the invention discloses a complex microbial agent Bwhich comprises the Pseudomonas putida, and Nitrosomonas europaea and Nitrobacter winogradsky with a nitrification function, synergistically degrades petroleum and petroleum products, and increases the ammonium nitrogen removal rate at the same time.

Description

technical field [0001] The invention relates to the technical field of pollution control, in particular to a bacterial strain, a composite bacterial agent, a preparation method and application thereof for bioremediation of petroleum-contaminated water bodies. Background technique [0002] As a fossil energy, petroleum has complex and diverse components. Once leaked into the environment, it is difficult to completely remove it, and it is highly toxic and carcinogenic. Traditional oil pollution remediation methods mainly rely on physical and chemical means such as containment, adsorption, dispersants, and surfactants to recover the leaked oil. The advantage is that the remediation can start quickly and is suitable for emergency treatment, but it cannot completely remove the residual low-lying oil. Concentration oil. The microbial remediation method can make up for this deficiency, and use the characteristics of oil-degrading bacteria to use oil as a carbon source and energy s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34B09C1/10C12R1/40C12R1/07C12R1/10C12R1/125C12R1/01C02F101/32
CPCB09C1/10C02F3/34C02F2101/32C12N1/20C12N1/205C12R2001/40
Inventor 万瑶佳林彦徐王璐琛
Owner WUHAN SHUIZHIGUO ENVIRONMENTAL PROTECTION TECH CO LTD
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