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A three-stage functional degradable artificial ligament regeneration scaffold and its preparation method

An artificial ligament, a three-stage technology, applied in the field of three-stage functional degradable artificial ligament and its preparation, can solve the problems that physiological tendon-bone healing cannot be achieved, ligament repair scaffolds have not yet appeared, and ligament regeneration cannot be induced. , to achieve good histocompatibility and biomechanical properties, simple preparation technology ideas, and effective preparation technology ideas

Active Publication Date: 2021-12-10
SHANGHAI SIXTH PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Based on the above, we conclude that the main problems faced by ligament regeneration are: there are defects such as inability to induce ligament regeneration, failure to achieve physiological tendon-bone healing, and easy failure.
However, so far, there have been no reports on the preparation of ligament repair scaffolds using electrospinning technology, braiding technology, and brittle fracture-casting-mold forming-freeze-drying technology.

Method used

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  • A three-stage functional degradable artificial ligament regeneration scaffold and its preparation method
  • A three-stage functional degradable artificial ligament regeneration scaffold and its preparation method

Examples

Experimental program
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Effect test

preparation example Construction

[0035] see figure 1 As shown, the present invention provides a method for preparing a three-stage functional degradable artificial ligament regeneration scaffold, which specifically includes the following steps:

[0036] Step 1: Dissolving or hot-melt mixing the organic polymer material and silk fibroin at a mass ratio of 1:1 to 4:1 to obtain a composite spinning solution or a mixed drawing melt, which is ready for use;

[0037] Step 2: heat-melt silk fibroin, mesoporous nanoparticles loaded with tendon-bone healing growth factor, and tendon-bone healing growth factor at a mass ratio of 75:20:5 to 95:4:1 Mix and prepare a uniformly mixed casting solution with a certain concentration;

[0038] Step 3: Spinning or melt-drawing the composite spinning solution or molten drawing melt prepared in step 1) respectively to obtain nanofiber yarns or long monofilaments of different diameters and lengths;

[0039] Step 4: braiding the nanofiber yarn or long monofilament prepared in step...

Embodiment 1

[0057] refer to figure 1 As shown in the preparation process, this embodiment provides a method for preparing a three-stage functional degradable artificial ligament regeneration scaffold, which specifically includes the following steps:

[0058] Step 1: Weigh poly(glycolic acid) (PGA) / tussah silk fibroin (SF) with a mass ratio of 2:1 on an analytical balance, dissolve it in hexafluoroisopropanol at 25°C, and stir to dissolve until A clear homogeneous solution with a mass volume ratio (g / mL) of 15% was obtained;

[0059] Step 2: Dissolve the three components of silk fibroin, mesoporous nanoparticles loaded with tendon-bone healing growth factor, and tendon-bone healing growth factor according to the mass ratio of 75:20:5, and prepare a mass volume concentration of 20 % of casting liquid;

[0060] Step 3: The mixed solution spinning solution is then subjected to trumpet-conjugated electrospinning to obtain a PGA / SF composite nanofiber yarn with a diameter of 300 μm;

[0061]...

Embodiment 2

[0068] see continue reading figure 1 As shown in the preparation process, this embodiment provides a method for preparing a three-stage functional degradable artificial ligament regeneration scaffold, which specifically includes the following steps:

[0069] Step 1: Weigh poly-L-lactic acid (PLLA) / tussah silk fibroin (SF) with a mass ratio of 3:1 on an analytical balance, heat and stir at 90°C to obtain a mixed drawing melt, and set aside;

[0070] Step 2: Dissolve the three components of silk fibroin, mesoporous nanoparticles loaded with tendon-bone healing growth factor, and tendon-bone healing growth factor according to the mass ratio of 80:16:4, and prepare a mass volume concentration of 20 % of casting liquid;

[0071] Step 3: Melt and draw the molten wire drawing solution to obtain a PLLA / SF composite long monofilament with a diameter of 150 μm;

[0072] Step 4: After pulling the PLLA / SF composite long monofilament, use 16 parallel threads, twisting and knitting techno...

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Abstract

The invention discloses a three-stage functional degradable artificial ligament regeneration bracket and a preparation method thereof. The preparation method comprises: preparing a composite spinning solution or a mixed drawing melt; preparing a casting solution; preparing nanofiber yarns or long strands silk; weaving tubular textile; heat setting tubular textile in assembled mold; making first section composite with cylindrical mold; making first and second section composite with cylindrical mold; Then the casting solution is continuously cast into a cylindrical mold to obtain a three-stage scaffold complex; finally, the three-stage scaffold complex is electrospun to obtain the final product. The three-stage functional degradable artificial ligament regeneration scaffold prepared by the present invention can perform its duties structurally, has good biomechanical properties and biocompatibility, and can be used for diseases such as rotator cuff, tendon, and ligament injuries regenerative treatment; and its preparation method is simple and efficient, low in price, and has good application prospects.

Description

technical field [0001] The invention relates to the technical field of a three-stage functional degradable artificial ligament and its preparation method, in particular to a mechanically enhanced ligament regeneration scaffold for rapidly promoting tendon-bone and preventing postoperative loosening and its preparation method. Background technique [0002] Effective repair of the anterior cruciate ligament (ACL) after injury is currently a major clinical problem, mainly due to the weak self-healing ability of the completely ruptured ACL, and the high rate of postoperative loosening and re-rupture. There are two main reasons for the failure of ACL reconstruction. One is that the intrinsic characteristics of the graft used for reconstruction cannot effectively match the autologous ligament tissue. For example, allogeneic tendon, autologous tendon, and artificial ligament are all alternatives to the damaged ligament. , but there is the rejection of the allograft tendon and the s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/22A61L27/18A61L27/10A61L27/12A61L27/50A61L27/54A61L27/58
CPCA61L27/227A61L27/18A61L27/10A61L27/12A61L27/50A61L27/54A61L27/58A61L2430/10A61L2300/414A61L2400/12A61L2300/412A61L2300/602C08L89/00C08L67/04
Inventor 赵金忠朱同贺蒋佳燕晓宇康育豪王立人徐俊杰李亚民
Owner SHANGHAI SIXTH PEOPLES HOSPITAL
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