Citrus Sunki Hort. Brewing, Preparation Method Thereof, and Medical Application Thereof
A lime, high-performance liquid chromatography technology, applied in pharmaceutical formulations, antipyretics, drug combinations, etc., can solve problems such as lack of overall applicability, short production period, and sour fruit taste
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Embodiment 1
[0044] Embodiment 1: the preparation of sour orange brewing product of the present invention.
[0045] Take about 4.5 kilograms of fresh sour oranges (the sour tangerines used in this experiment have a sugar-acid ratio of 1.86 after being measured), wash them twice with water, then crack the whole fruit, and put them into factory-type juicing equipment for juice extraction and deslagging . The residual seeds in the juiced pomace were then removed by a deseeding machine to obtain about 1 kg of seedless pomace. Then, the obtained seedless pomace was sealed and stored in a culture room, and reacted at 60° C. for 48 hours (aging reaction). Then, the pomace was dehumidified and dried at 50°C for 4 hours; then, using radio frequency assisted hot air sterilization technology, the semi-dried pomace obtained above was processed under the conditions of hot air 50°C, frequency 40.68MHz, and electrode plate spacing 130cm Treat for 120 seconds and proceed to the sterilization step. Acco...
Embodiment 2
[0049] Embodiment 2: Detection of the total polyphenol content, total flavonoid content and anti-inflammatory effect of the sour orange brewed product of the present invention.
[0050] Detect the content of total polyphenols and total flavonoids contained in the sour orange brewed product obtained in this experiment. The detection methods used in this experiment are roughly as follows:
[0051] Analysis of the content of total polyphenolic compounds: take 1mL of 0.2N Folin reagent, add 0.2mL of 25 to 200ppm of the sample to be tested or gallic acid and 0.8mL of 7.5% sodium carbonate solution in the test tube, shake and mix evenly, and set the temperature at room temperature Stand still for 30 minutes, and measure the change in absorbance at a wavelength of 765 nm. The measured concentration of the sample to be tested is converted to the concentration of gallic acid.
[0052] Analysis of total flavonoid content: take the solution to be tested (100 mL), add 30 mL of 5% sodium...
Embodiment 3
[0060] Embodiment 3: detect the main chemical components contained in the sour orange brewed product of the present invention
[0061] In order to further confirm the main chemical components of the sour orange brewed product of the present invention, high performance liquid chromatography equipment was used to analyze the chemical index components for the above-mentioned samples 1 and 5. The experimental conditions of this high performance liquid chromatography are as follows:
[0062] Analyze at a flow rate of 1mL / min and an absorption wavelength of 320nm for 60 minutes, and the analysis conditions are as follows: 0.1 to 50 minutes: 15%→90% acetonitrile aqueous solution (ACN) containing 0.1% formic acid; 50.1 to 55 minutes: 90% ACN; 55.1 to 60 minutes: 90% → 15% ACN.
[0063] See figure 1 . The accompanying drawing shows the fingerprint (320nm) of the sour orange pomace brewing product after HPLC qualitative analysis, flavonoids 1-5 represent naringin (naringin (1); 17.1 ...
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