PCR primers, PCR methods and kits for detecting Staphylococcus cocci

Abstract

The present application belongs to the field of detection of pathogenic microorganisms, and relates to PCR primers, detection methods, and applications of the detection methods for the detection of Staphylococcus korea. High detection primers, fluorescent quantitative PCR methods and detection kits. The primers include an upstream primer and a downstream primer, the nucleotide sequence of the upstream primer is the sequence shown in SEQ ID NO.1, the nucleotide sequence of the downstream primer is the sequence shown in SEQ ID NO.2, and the The PCR method includes at least obtaining the upstream primer and the downstream primer by Primer Premier 5 design according to the Staphylococcus korekii heat shock protein HSP60 gene, and the kit provides at least the upstream primer and the downstream primer, or using the PCR method.

Description

technical field [0001] The present application belongs to the field of detection of pathogenic microorganisms, and relates to the detection of Staphylococcus korekis, in particular to PCR primers for detecting Staphylococcus korekis, a detection method and the application of the detection method. Specifically, the present application provides a pair of detection primers, a fluorescent quantitative PCR method and a detection kit with good specificity and high sensitivity for Staphylococcus korsi. Background technique [0002] Staphylococcal infections are zoonotic infectious diseases caused by pathogenic bacteria within the genus Staphylococcus. Bacteria of this genus are widely present in the skin and mucous membranes of the respiratory tract, upper gastrointestinal tract, and urogenital tract of mammals. They generally invade the body through wounds, and most of them will cause purulent diseases and systemic sepsis in different parts of the body; in addition, staphylococcal...

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Problems solved by technology

[0004] In the prior art, most of the identification methods of the bacteria are biochemical identification by isolation and culture of the bacteria, and at the same time, they are determined according to the morphological characteristics and biochemical characteristics of the bacteria. However, the method of first culturing and then identification takes a long period, usually at least 4-4 years. 7 working days; the operation requirements for the experimenter are high, and the experimenter needs to judge and identify the size, shape, color, shape, etc. of the colony; moreover, the morphological characteristics of the bacteria in the Staphylococcus genus are similar and difficult to distinguish, so the traditional detection method It is not conducive to early identification, rapid detection or control of the infection of this bacteria

Moreover, the sensitivity of this method is low, and there may be missed detection; it needs to be combined with the results of biochemical identification to determine whether there is a strain

[0005] With the development of biotechnology, molecular biology techniques are more and more applied to the detection of pathogenic microorganisms. The existing methods also include the PCR method, which is based on the amplification of the bacterial 16s rDNA gene and combined with sequencing. However, There is no specific PCR method for Staphylococcus kosheri

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