Modified type-I collagen, modification method, and collagen gel prepared from modified type-I collagen

Abstract

The invention belongs to the field of medical biological materials, and particularly discloses a modified type-I collagen, a modification method, and collagen gel prepared from the modified type-I collagen. The modification method comprises the steps of (1) uniformly mixing weak acid with a type-I collagen at the temperature of 2-10 DEG C to prepare an acid collagen solution; (2) utilizing a phosphate buffering solution for dialysing the acid collagen solution to obtain a neutral collagen solution; (3) removing bubbles in the neutral collagen solution; (4) utilizing a rotational rheometer forcutting the neutral collagen solution after bubble removal under a liquid seal condition, and conducting standing after cutting to prepare the modified type-I collagen. According to the modified type-I collagen, the modification method, and the collagen gel prepared from the modified type-I collagen, the collagen is modified through mechanical cutting. The method is simple and efficient, three-helix structures of collagen molecules can be reduced on the basis that other substances do not need to be introduced, and random coil structures are added, so that fibers of the assembled collagen are controllable in diameter, pore and viscoelasticity.

Description

technical field [0001] The invention belongs to the field of medical biomaterials, and more specifically relates to a modified type I collagen, a modification method and a collagen gel prepared by using the modified type I collagen. Background technique [0002] Collagen, also known as collagen, is a very important protein resource with a wide range of sources. It mainly exists in animal skin, cartilage, bone, ligament and blood vessels, and is the main component of extracellular matrix. Collagen is a white opaque, unbranched and insoluble fibrous protein, which usually accounts for 25%-30% of the total protein in the animal body, and plays an important role in supporting, protecting, combining and forming interface partitions for the animal body and its organs effect. At present, 28 kinds of collagen have been discovered one after another, among which type I collagen is the type of collagen with the highest content and the widest distribution. Typical type I collagen cons...

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Problems solved by technology

[0008] The purpose of the present invention is to provide a method for physical modification of collagen by mechanical shearing, which can be simple, efficient and safe, aiming at the problems of complex collagen modification operations, low modification efficiency, and difficulty in avoiding the introduction of exogenous toxic substances. It can precisely regulate the molecular structure of collagen, and can adjust the assembly behavior of collagen molecules and the properties of the gel after assembly, so as to meet the application requirements of collagen-based materials in the field of biomedical materials

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