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Preparation method and application method of phosphorylated protein enrichment material

A technology for phosphorylating proteins and application methods, applied in the preparation methods of peptides, chemical instruments and methods, separation methods, etc., can solve the problems of weak interaction, easy loss of metal ions, etc., and achieve large adsorption capacity, low cost, and separation. Simple process effect

Active Publication Date: 2019-10-11
NORTHEASTERN UNIV
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Problems solved by technology

However, the traditional metal chelating reagents, iminodiacetic acid and nitrilotriacetic acid, have weak interactions with solid-phase supports, and metal ions are easily lost. Therefore, a simple chelating reagent was chosen to develop a new The adsorption material is very important for the enrichment of phosphorylated proteins

Method used

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  • Preparation method and application method of phosphorylated protein enrichment material
  • Preparation method and application method of phosphorylated protein enrichment material
  • Preparation method and application method of phosphorylated protein enrichment material

Examples

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Embodiment 1

[0069] Embodiment 1: the synthesis of titanium chelated polydopamine-polyoxometalate composite material

[0070] (1) Dissolve 33g of sodium tungstate dihydrate in 30mL of water, and add 26.5mL of 85% phosphoric acid. After magnetic stirring for 30 min, the mixed solution was transferred to a 150 mL reactor, and subjected to hydrothermal reaction at 120 °C for 12 h. After the reaction kettle was cooled to room temperature, there were light green crystals on the polytetrafluoroethylene wall of the liner, the product was taken out, 15mL of water was added, and then 10g of potassium chloride was added to form a white precipitate. After stirring for 1 h, until the mass of the white precipitate no longer changed, the precipitate was collected by suction filtration, washed with methanol three times, and dried at 60°C. Finally, dissolve the solid in 30mL of water at 70°C, the solid dissolves, and white crystals precipitate out after cooling, filter under reduced pressure, and dry at ...

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Abstract

The invention relates to a preparation method and application method of a phosphorylated protein enrichment material. The preparation method comprises the steps of preparing a corresponding polyoxometalate during preparation; polymerizing dopamine and polyoxometallate to form composite microspheres as a carrier; fixing metal ions to the surfaces of the microspheres to obtain an enrichment materialproduct. An obtained final product is used as an adsorption material for selective separation enrichment of phosphorylated protein. The applied dopamine has good water solubility and is free of toxinand harm and excellent in metal bonding capability. In addition, Pi bonds exist in both the polyoxometallate and phosphate radical groups so that a Pi accumulation effect can be achieved, and the adsorption effect on phosphorylated protein is further improved. The material has the advantages of simple synthesis process, low cost, high selectivity and the like.

Description

technical field [0001] The invention belongs to the technical field of protein separation and enrichment, and in particular relates to the preparation and application method of a phosphorylated protein enrichment material. Background technique [0002] Phosphorylated protein, as a very important post-translational modification protein, plays an important role in many biological processes, such as signal transduction, molecular recognition, cell differentiation and metabolism. Abnormal protein phosphorylation levels are involved in the pathogenesis of many human diseases, such as cancer, heart disease, and cardiovascular disease. However, the low content of phosphorylated proteins in biological samples limits their study. Therefore, in order to study the phosphorylation modification of proteins in depth, it is necessary to use enrichment and purification methods to separate low-abundance phosphorylated proteins from complex real samples. [0003] Immobilized metal ion affin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/281B01J20/30B01D15/20C07K1/22
CPCB01D15/20B01J20/281C07K1/22
Inventor 陈帅王蒙蒙王建华于永亮
Owner NORTHEASTERN UNIV
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