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Bacillus subtilis strain and application thereof

A Bacillus subtilis and bacterial strain technology is applied to the Bacillus subtilis strain and its application field to achieve the effects of broad-spectrum antibacterial ability and broad application prospects

Active Publication Date: 2019-09-24
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] For this reason, the embodiment of the present invention provides a kind of Bacillus subtilis bacterial strain and its application, obtains better effect in inhibiting Escherichia coli, Salmonella typhimurium, Staphylococcus aureus, Aspergillus niger, and solves the problem of antibacterial cyclolipopeptide in the prior art. content problem

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  • Bacillus subtilis strain and application thereof
  • Bacillus subtilis strain and application thereof
  • Bacillus subtilis strain and application thereof

Examples

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Embodiment 1

[0035] This example provides a strain of Bacillus subtilis, which was deposited in the General Microorganism Center of China Committee for the Collection of Microorganisms on October 10, 2018, with the preservation number: CGMCC NO.16568.

[0036] In addition, this embodiment also provides a high-density fermentation broth of Bacillus subtilis containing metabolites of the Bacillus subtilis strain.

[0037] Further, this example provides the application of a high-density fermentation broth of Bacillus subtilis in inhibiting Escherichia coli, Salmonella typhimurium, Staphylococcus aureus, and Aspergillus niger.

[0038]The Bacillus subtilis strain in this example has been preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee, and can effectively inhibit Escherichia coli, Salmonella typhimurium, Staphylococcus aureus, and Aspergillus niger, and produce more antibacterial substances ; The high-density fermented broth of Baci...

Embodiment 2

[0040] The present embodiment provides a kind of preparation method of Bacillus subtilis high-density fermented liquid, comprises the following steps:

[0041] Step 1. Preparation of bacterial strain seed solution: activating the Bacillus subtilis, taking the activated Bacillus subtilis single colony and marking it on the medium, then sealing it with glue and putting it into an incubator for cultivation to obtain an activated bacterial strain, Transferring the activated bacterial strain into the culture medium to obtain the described strain seed solution;

[0042] Step 2, preparation of high-density fermented liquid: inoculate the described strain seed liquid in the container that high-density culture medium is housed and cultivate, obtain bacterial suspension, collect supernatant after described bacterial suspension is centrifuged, The supernatant is filtered and sterilized to obtain the high-density fermentation broth of Bacillus subtilis.

[0043] Preferably, the preparati...

Embodiment 3

[0051] The present embodiment provides a kind of preparation method of Bacillus subtilis high-density fermented liquid comprising the steps:

[0052] 1) Preparation of strain seed solution: activate Bacillus subtilis on NA plate medium, take a single colony and streak it on NA medium, seal the glue upside down and place it in a 37°C incubator for 24 hours to obtain the activated strain, and transfer the bacteria with Transfer the activated strains to LB medium, culture at 37°C and 170r / min for 24 hours, and store the prepared strains at 4°C for later use;

[0053] 2) Preparation of high-density fermented liquid: Inoculate the strain seed liquid prepared in step 1) in an Erlenmeyer flask of high-density medium with an inoculum size of 5% (V / V). Under the condition of 24 hours of culture, the number of live bacteria of Bacillus subtilis CGMCC NO.16568 reached 4.8×10 11 CFU / mL. Obtain the bacterial suspension, centrifuge the bacterial suspension at 9000r / min, take the supernata...

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Abstract

The embodiment of the invention discloses a bacillus subtilis strain and application thereof. The bacillus subtilis strain is preserved in the China General Microbiological Culture Collection Center on October 10th, 2018, and the preservation number is CGMCC NO.16568. The bacillus subtilis strain can effectively inhibit escherichia coli, salmonella typhimurium, staphylococcus aureus and aspergillus niger, and more antibacterial substances are generated; bacillus subtilis high-density fermentation liquor containing bacillus subtilis strain metabolite has broad-spectrum antibacterial capability, contains high-content cyclic lipopeptide, and has wide application prospects. The bacillus subtilis high-density fermentation liquor also has good effects in inhibiting escherichia coli, salmonella typhimurium, staphylococcus aureus and aspergillus niger.

Description

technical field [0001] The embodiments of the present invention relate to the technical field of microbial application, and in particular to a strain of Bacillus subtilis and its application. Background technique [0002] Bacillus subtilis, a thermophilic, aerobic, spore-forming, G+ rod-shaped bacterium, has long been used to ferment soybean foods and is widely used in the production of industrial enzymes and chemicals. It can produce a variety of antibiotics and enzymes. In terms of biological control, it is often used to control rice blast, wheat sheath blight and rape sclerotinia. In recent years, it has also been applied to fruit diseases, such as strawberry powdery mildew and mango anthracnose illness etc. [0003] Bacillus subtilis has a broad antibacterial spectrum, mainly because it can secrete a variety of antagonistic substances, including low-molecular-weight antimicrobial peptides synthesized by non-ribosomes and macromolecular protein bacteriocins synthesized b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P1/00A01P3/00C12R1/125
CPCC12N1/20C12R2001/125C12N1/205A01N63/10
Inventor 范俊峰张波王婕林珅竹许琳琳
Owner BEIJING FORESTRY UNIVERSITY
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