Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of miR-584-5p as biomarker for acute respiratory distress syndrome

A kind of acute respiratory distress, biomarker technology, applied in the field of biotechnology and medicine, can solve the problems of patient trauma, inconvenient material collection, etc., to achieve the effect of assisting ARDS pathological identification and clinical diagnosis, and good clinical application prospects

Pending Publication Date: 2019-09-17
杭州市西溪医院
View PDF2 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In traditional detection methods, the detection of miRNA expression levels generally requires collection of patient lesions, which is very inconvenient and traumatic to patients.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of miR-584-5p as biomarker for acute respiratory distress syndrome
  • Application of miR-584-5p as biomarker for acute respiratory distress syndrome
  • Application of miR-584-5p as biomarker for acute respiratory distress syndrome

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The ARDS serum samples used in this example (including 11 serum samples at the onset of ARDS patients and 10 serum samples at the time of death of ARDS patients) came from patients who received treatment at Xixi Hospital, Hangzhou City, Zhejiang Province between December 2013 and February 2016. Whole blood from patients with ARDS. Enrolled patients are required to be older than or equal to 18 years old, with sound heart function, and non-pregnant and lactating patients. The normal serum control samples used in the present invention come from healthy volunteers (20 cases).

[0029] Reagents used in this example, such as chloroform, isopropanol, ethanol, DNA polyacrylamide gel electrophoresis reagents and other conventional biochemical reagents, were purchased from Shanghai Sangong Company; PCR amplification reagents (containing 10 × buffer, dNTPs mixture) and reagents for reverse transcription, such as RNase inhibitor, M-MLV reverse transcriptase, 5×M-MLV buffer and dNTP...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses application of miR-584-5p as a biomarker for acute respiratory distress syndrome. The invention discloses for the first time that miR-584-5p is associated with the acute respiratory distress syndrome. The analysis of the invention finds that the expression of miR-584-5p in normal serum, the serum of ARDS patients at the onset of ARDS and the serum of the ARDS patient in death is significantly different. Therefore, the invention infers and verifies that miR-584-5p can be used as a serological biomarker for ARDS, and can be singly used for rapid non-invasive screening of ARDS patients, and assisting in pathological identification and clinical diagnosis of ARDS; the application of miR-584-5p is convenient to judge the incidence degree of ARDS patients at the serum level, and predict the development direction of ARDS condition to ensure that patients receive active treatment; and the application of miR-584-5p is also convenient for medical personnel to take appropriate medical treatment measures according to the diagnosis results, and has good clinical application prospects.

Description

technical field [0001] The invention belongs to the fields of biotechnology and medicine, and in particular relates to the application of miR-584-5p as a biomarker for acute respiratory distress syndrome. Background technique [0002] Acute respiratory distress syndrome (ARDS) is caused by intrapulmonary and / or extrapulmonary causes, and its typical feature is refractory hypoxemia. It is a clinical syndrome and the mortality rate of patients is extremely high. There are many causes of ARDS, and the pathogenesis of different etiologies is different, and the morbidity and mortality rate of the same individual exposed to different environmental factors are also different. The most complicated thing is that the disease will change over time. changes happened. The clinical manifestations are mostly acute onset, respiratory distress, and hypoxemia that is difficult to correct with conventional oxygen therapy. Regarding the identification of ARDS, the "Berlin Definition" is often ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883
CPCC12Q1/6883C12Q2600/158C12Q2600/166C12Q2600/178
Inventor 张思泉丁先锋洪一诺周可欣
Owner 杭州市西溪医院
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com