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Application of aucklandia-coptis pill extract in preparing drugs for treating intestinal flora alternation diseases

A technology of intestinal flora imbalance and Xianglian pills, which is applied in the direction of drug combination, plant/algae/fungus/moss components, pharmaceutical formulations, etc., can solve the problems that the effect and mechanism have not been reported in the literature, and achieve the improvement of intestinal microbiology. Environment, improving symptoms, increasing the effect of beneficial gut microbiota

Inactive Publication Date: 2019-09-17
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Modern pharmacological studies have shown that Xianglian Wan has a wide range of effects such as antibacterial, anti-inflammatory and analgesic effects, but there are no literature reports on its effect and mechanism in the treatment of antibiotic-associated diarrhea

Method used

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  • Application of aucklandia-coptis pill extract in preparing drugs for treating intestinal flora alternation diseases
  • Application of aucklandia-coptis pill extract in preparing drugs for treating intestinal flora alternation diseases
  • Application of aucklandia-coptis pill extract in preparing drugs for treating intestinal flora alternation diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Prepare Xianglian pill extract, its method is as follows:

[0025] Weigh 100g of the crude drug of Xianglian Pills, first use 85% ethanol to reflux extract for 3 times, collect the filtrate and filter residue respectively after filtration, recover ethanol from the filtrate under reduced pressure to obtain an ethanol extract for later use, and extract the filter residue with an appropriate amount of water at 90°C for 3 Once, each 1 hour, filter and collect the filtrate, obtain the water extract after suitably concentrating, combine the water extract and the ethanol extract to obtain a mixed solution, adjust the volume of the mixed solution with water to be 100ml, and then obtain the Xianglian Pills extract, Every 1ml of the extract is equivalent to 1g of Xianglianwan crude drug.

Embodiment 2

[0027] The Xianglianwan extract prepared in Example 1 is used to treat diarrhea caused by antibiotics in mice:

[0028] Take 30 mice with the same growth status, half male and half male, and randomly divide them into control group, model group, and Hexianglian pill treatment group, with 10 mice in each group. The mice in the model group and the Xianglian pill treatment group were intragastrically administered the mixture of cefuroxime and levofloxacin (260+200mg / kg.bw), twice a day, for 5 consecutive days, and the control group was intragastrically administered the same volume of physiological brine. Beginning on the 6th day, the control group and the model group were administered with normal saline, and the Xianglian pill group was administered with the Xianglian pill extract (4.5g / kg.bw) prepared in Example 1, twice a day for 5 consecutive days.

[0029] Observe and evaluate the diarrhea state of the mice: soft, thin, and sticky stools are typical symptoms of diarrhea, and ...

Embodiment 3

[0037] The Xianglian pill extract prepared in Example 1 was used to treat diarrhea caused by antibiotics in mice, and studied its effect on the type and content of microorganisms contained in mouse feces:

[0038] Take the feces of mice in the control group, model group and Xianglian pill group 12 hours after the last administration in Example 2, and use 16SrRNA high-throughput sequencing technology to analyze the diversity of microbial colonies. The results are shown in Tables 2 and 3.

[0039] Table 2 The relative abundance of microbial flora at the family level (%)

[0040]

[0041] From the relative abundance of the microbial flora in the family level in Table 2, the microbial diversity in the feces of the mice was extremely reduced after oral administration of antibiotics, and the proportion of Enterococcus family reached 98.78%, an increase of 123375% compared with the normal group. %; After treatment with the extract of Xianglian Pills, the microorganisms of this fam...

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Abstract

The invention relates to application of an aucklandia-coptis pill extract in preparing drugs for treating intestinal flora alternation diseases, especially for intestinal microflora alternation caused by antibiotics, and belongs to the field of application of traditional compound Chinese medicine preparations. Firstly, an ethyl alcohol extracting solution and residues are extracted by adopting ethyl alcohol refluxing from a raw aucklandia-coptis pill, then the residues are extracted with water to obtain an aqueous extracting solution, and finally, the ethyl alcohol extracting solution and the aqueous extracting solution are combined to obtain the aucklandia-coptis pill extract. The aucklandia-coptis pill extract as a drug for treating the intestinal flora alternation diseases has a good curative effect, and can significantly ameliorate the symptoms of antibiotic-associated diarrhea while significantly increasing intestinal beneficial microbiota and improving the intestinal microenvironment.

Description

technical field [0001] The invention belongs to the application field of traditional compound traditional Chinese medicine preparations, and in particular relates to the application of Xianglian pill extract in the preparation of medicines for treating intestinal flora disorders. Background technique [0002] When antibiotics are used to treat diseases, a serious side effect often occurs, that is, intestinal flora imbalance, especially broad-spectrum antibiotics have a wide range of killing or inhibiting effects on intestinal microorganisms, causing intestinal bacterial imbalance is more common and serious. Intestinal bacterial imbalance can lead to various diseases, such as obesity, diabetes and diarrhea. The onset of diarrhea is fast and occurs during or at the end of treatment. It is also called antibiotic-associated diarrhea. The incidence rate varies from 5 to 39 depending on the antibiotic. %. Clinically, there are two types of antibiotic-associated diarrhea: diarrhe...

Claims

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Application Information

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IPC IPC(8): A61K36/754A61P1/00A61P1/12
CPCA61K36/285A61K36/718A61K36/754A61K2236/331A61K2236/333A61P1/00A61P1/12A61K2300/00
Inventor 袁吕江杨璐嘉黄洁瑶刘丹宁
Owner SOUTHWEST UNIVERSITY
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