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A flocculant-producing strain and its application in shrimp biofloc culture and dye decolorization

A technology of flocculant and bacteria production, applied in biochemical equipment and methods, applications, microorganisms, etc., can solve the problems of nutrient loss, increased incidence of shrimp, and surrounding environmental pollution, etc., and achieve high cellulase and xylan Enzyme activity, broad application prospects, and the effect of reducing production costs

Active Publication Date: 2022-01-18
NINGBO UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, with the increase in the area of ​​prawn farming and the improvement of the degree of intensification, coupled with the increase in the density of prawn farming, a large amount of feed needs to be invested, resulting in a large accumulation of nitrogen in the aquaculture water and the deterioration of water quality.
At the same time, a large number of water changes lead to the loss of nutrients and pollution to the surrounding environment, causing a serious imbalance in the natural ecosystem environment, and the incidence of shrimp is increasing day by day

Method used

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  • A flocculant-producing strain and its application in shrimp biofloc culture and dye decolorization
  • A flocculant-producing strain and its application in shrimp biofloc culture and dye decolorization
  • A flocculant-producing strain and its application in shrimp biofloc culture and dye decolorization

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1. Screening of flocculant-producing bacteria

[0029] Take 5.0 g of biofloc from the Ninghai shrimp biofloc culture pond, resuspend in 50 ml of sterile PBS buffer, boil for 2 minutes, place on a constant temperature shaker at 30±2°C, and incubate at 180-220 rpm for 30 minutes. Then use the standard dilution plate coating method to spread on 1 / 2 of the 2216E solid medium, and culture it upside down in a constant temperature incubator at 30±2°C for 2-4 days. Pick a single colony and number it according to the shape and color of the colony. The picked single colonies were purified by drawing again, placed in 1 / 2 of the 2216E liquid medium, and cultured in a constant temperature shaker at 30±2°C, 180~220 rpm for 2~4 days, and the fermented liquid was collected and centrifuged at 12000g for 3 minutes Finally, the flocculation efficiency of the supernatant was measured by kaolin suspension, and the strain NBU10 with higher flocculation efficiency was selected for subsequent ...

Embodiment 2

[0046] Utilize the bacterial strain that above-mentioned embodiment 1 obtains is applied to shrimp biofloc culture pattern

[0047] This experiment was completed in the Xianxiang breeding base in Ningbo City, Zhejiang Province. A total of 10 600 L (diameter 1 m, height 0.8 m) Litopenaeus vannamei breeding ecological experiment barrels were set up, of which 5 barrels were used as controls and 5 were used as wild animals. Xanthomonas napus NBU10 addition group. The shrimp seedlings were put in uniformly, about 200 seedlings were placed in each experimental bucket, and the experiment was formally started 2 days after the seedlings were released. The strain addition method is: Xanthomonas campestris NBU10 is cultured with 2216E medium until the bacterial liquid OD 600 When the absorbance value was about 1.0, 1 L of bacterial liquid and 500 g of rice straw powder were added to the addition group, and the control group was replaced by sterile medium. Feed 3 times a day (7:00, 12:0...

Embodiment 3

[0049] Fermentation Broth Preparation with Flocculation Activity and Its Application in Dye Removal

[0050] Inoculate the strain with the preservation number CGMCC No.16936 into the seed culture solution for seed culture, then inoculate the seed solution into the fermentation culture solution containing 0.25-1.0 wt% rice straw for fermentation and culture; place the fermentation solution at 4°C Overnight, carefully remove the precipitate, and the supernatant is the liquid flocculant with flocculation.

[0051] Take 50 mL of 50 mg / L Coomassie Brilliant Blue dye, add 0.5, 1.0, 2.0 and 3.0 mL of the above-mentioned liquid flocculants, and then add 1 mL of 10 g / L calcium chloride solution, and stir rapidly for 1 min. After stirring slowly for 30 s, let stand at room temperature for 30 min, take the supernatant and measure its absorbance value B at 563 nm. With the addition of 0.5, 1.0, 2.0 and 3 mL of distilled water as controls, the absorbance value A at 563 nm was measured, an...

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Abstract

The invention discloses a flocculant-producing bacterium and its application in prawn biofloc culture and dye decolorization, which is characterized in that the bacterium is Xanthomonas campestris NBU10, which was preserved in China's microbial strains on December 12, 2018 The General Microorganism Center of the Preservation Management Committee, the deposit number is CGMCC No.16936, and the method for preparing the bioflocculant is as follows: inoculate the strain into the seed culture solution for seed solution cultivation, and then inoculate the seed solution into rice containing 0.5 wt% Fermentation and culture in straw fermentation culture liquid; place the fermentation liquid at 4°C overnight, carefully remove the precipitate, the supernatant is a liquid flocculant with flocculation, the advantage is that it can degrade biomass and produce biological flocculant at the same time, Adding the invented bacterial strain in the culture pond can help to form biofloc better and faster, improve the water quality of the prawn culture and increase the yield of the prawn.

Description

technical field [0001] The invention belongs to the field of microbial preparations, and in particular relates to a flocculant-producing bacterium isolated from bio-floc culture water and a method for its application in prawn bio-floc culture and dye decolorization. Background technique [0002] Shrimp farming is one of the most representative industries in my country's aquaculture industry. In recent years, with the increase in the area of ​​shrimp farming and the improvement of intensification, coupled with the increase in the density of shrimp farming, a large amount of feed needs to be invested, resulting in a large accumulation of nitrogen in the farming water and deterioration of water quality. At the same time, a large number of water changes cause the loss of nutrients, and cause pollution to the surrounding environment, causing a serious imbalance in the natural ecosystem environment, and the incidence of shrimp is increasing day by day. Microorganisms in aquacultu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P1/04C02F3/34A01K61/59C12R1/64
CPCC12P1/04C02F3/34A01K61/59C12R2001/64C12N1/205Y02A40/81
Inventor 郭海朋张德民赵月季朱悦悦黄雷
Owner NINGBO UNIV
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