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Application of IRF4 gene in resisting schistosoma infection

An anti-schistosomiasis and gene technology, applied in the field of biomedicine, can solve the problem of few types of drugs for schistosomiasis infection, achieve great medicinal prospects and value, inhibit the formation of liver granulomas, and relieve symptoms.

Active Publication Date: 2019-09-13
GUANGZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Currently there is no effective vaccine to prevent schistosomiasis infection, and there are few effective drugs for the treatment of schistosomiasis infection

Method used

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  • Application of IRF4 gene in resisting schistosoma infection
  • Application of IRF4 gene in resisting schistosoma infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1 establishes the model mouse of schistosomiasis infection

[0024] The IRF4 knockout mouse infection group, the wild mouse infection group and the wild mouse control group (not infected with schistosome) were set up, with 5 mice in each group. Selection criteria for mice in each group: female, 6-8 weeks old, weighing about 20 g. Wherein, the mice in the wild mouse infection group and the wild mouse control group are of the same strain as the IRF4 gene knockout mice.

[0025] 1. Place Schistosoma japonicum-positive snails in dechlorinated water at 20-25°C under light to escape cercariae;

[0026] 2. Take mice from each group, and each mouse is infected with 40 cercariae of Schistosoma japonicum through abdominal skin;

[0027] 3. The schistosomiasis model mice can be obtained after 6 weeks of infection.

Embodiment 2

[0028] Embodiment 2 result detection

[0029] One of the main symptoms of schistosomiasis is hepatic granuloma and abnormal liver function. In this example, the role of IRF4 gene deletion in resisting schistosomiasis infection was evaluated by detecting the granuloma formation in the liver of each group of mice successfully modeled in Example 1 and the ALT level in the mouse serum.

[0030] 1. Granuloma formation in the liver of mice: 6 weeks after the mice in each group were infected with Schistosoma japonicum, the mice were taken out, bled through eyeballs, blood was collected by anticoagulant tubes, and then the mice were killed by cervical dislocation. The livers of the mice in each group were dissected, observed and photographed, and the results were as follows: figure 1 shown. Depend on figure 1 It can be seen that compared with the wild mouse control group, the livers of the wild mouse infection group and the IRF4 gene knockout mouse infection group all formed granul...

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Abstract

The invention discloses application of an IRF4 gene in resisting schistosoma infection, in particular to application of the IRF4 gene in preparing or screening a medicament for resisting the schistosoma infection. Meanwhile, the invention also discloses a medicament for resisting the schistosoma infection. The medicament comprises an agent capable of inhibiting or silencing the expression of the IRF4 gene. The invention utilizes IRF4 gene knock-out mice and wild mice to carry out experiments, finds that the IRF4 gene deletion can effectively inhibit the formation of liver granuloma of the micewith schistosoma infection, and obviously improves the liver function of the mice with schistosoma infection. The invention provides a new treatment target for resisting the schistosoma infection, provides a new thought for preparing and screening the medicament for resisting the schistosoma infection, and has great medicinal prospect.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to the application of IRF4 gene in resisting schistosome infection. Background technique [0002] Schistosoma japonicum, also known as schistosomiasis, belongs to the flatworm phylum, fluke class, polymorpha, and schistosome family. The schistosomes that parasitize the human body include Schistosoma japonicum (Schistosome japonicum), Schistosoma haematobium and Schistosoma mansoni. Schistosoma is distributed in 76 countries and regions in Asia, Africa and Latin America. In our country, only Schistosoma japonicum is prevalent, which was first discovered by the Japanese in Japan, so it is named Schistosoma japonicum. The intermediate host of schistosomiasis is the mollusk of the genus Oncomelania. Adult worms live in the mesenteric vein, and some eggs enter various organs with the blood flow, causing various symptoms, such as hepatomegaly, and in severe cases, causi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/00A61P33/12
CPCA61K45/00A61P33/12
Inventor 杨权黄俊冯源发金晨曦谢世豪杨靖雪谢红艳齐艳伟邱怀娜
Owner GUANGZHOU MEDICAL UNIV
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