Extraction method and product of selenium-enriched sturgeon fish bone peptide with anti-aging effect

An extraction method and sturgeon technology, which are applied in the field of extraction methods and products of selenium-enriched sturgeon bone peptides, can solve problems such as inability to utilize and develop sturgeon bones well, and achieve good absorption effect and obvious anti-aging effect. Effect

Active Publication Date: 2021-04-02
HUBEI YANGGUANG SCI & TECHCAL MEDICINES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the above defects or improvement needs of the prior art, the present invention provides an extraction method and product of sturgeon bone peptide with anti-aging effect. As an organic selenium source, it has obvious anti-aging effect, thus solving the technical problem that the existing technology cannot make good use and development of sturgeon fish bones

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  • Extraction method and product of selenium-enriched sturgeon fish bone peptide with anti-aging effect
  • Extraction method and product of selenium-enriched sturgeon fish bone peptide with anti-aging effect
  • Extraction method and product of selenium-enriched sturgeon fish bone peptide with anti-aging effect

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Embodiment 1

[0045] The extraction method of the sturgeon bone peptide with anti-aging effect provided by the present invention comprises the following steps:

[0046] (1) Pulverizing the sturgeon fish bone raw material through a 160-mesh sieve to obtain fish bone meal;

[0047] (2) Carrying out the first enzymolysis of the fish bone powder obtained in step (1), degrading the fish bone collagen skeleton, exposing selenium-enriched glycoprotein, and obtaining the crude product of enzymolysis;

[0048] Specific steps are as follows:

[0049] Add water to the fishbone powder obtained in step (1) according to the solid-to-liquid ratio (mass ratio) of 1:1 to make a suspension, then add collagenase and cellulase for enzymolysis, wherein the quality of collagenase is 0.5% of the quality of fishbone powder %, the mass of cellulase is 0.4% of the mass of fish bone meal, and the enzyme is hydrolyzed for 1.5 hours, during which the temperature is kept at 25 to 30°C with continuous stirring; heating ...

Embodiment 2

[0061] The extraction method of the sturgeon bone peptide with anti-aging effect provided by the present invention comprises the following steps:

[0062] (1) Pulverizing the sturgeon fish bone raw material through a 200-mesh sieve to obtain fish bone meal;

[0063] (2) Carrying out the first enzymolysis of the fish bone powder obtained in step (1), degrading the fish bone collagen skeleton, exposing selenium-enriched glycoprotein, and obtaining the crude product of enzymolysis;

[0064] Specific steps are as follows:

[0065] Add water to the fish bone powder obtained in step (1) according to the solid-to-liquid ratio (mass ratio) of 1:1.5 to make a suspension, then add collagenase and cellulase for enzymolysis, wherein the quality of collagenase is 0.4 of the quality of fish bone powder %, the mass of cellulase is 0.1% of the mass of fish bone powder, and the enzymolysis is carried out for 1 hour, during which the temperature is maintained at 25 to 30°C with continuous stir...

Embodiment 3

[0078] The extraction method of the sturgeon bone peptide with anti-aging effect provided by the present invention comprises the following steps:

[0079] (1) Pulverizing the sturgeon fish bone raw material through a 300-mesh sieve to obtain fish bone powder;

[0080] (2) Carrying out the first enzymolysis of the fish bone powder obtained in step (1), degrading the fish bone collagen skeleton, exposing selenium-enriched glycoprotein, and obtaining the crude product of enzymolysis;

[0081] Specific steps are as follows:

[0082] Add water to the fishbone powder obtained in step (1) according to the solid-to-liquid ratio (mass ratio) of 1:2 to make a suspension, then add collagenase and cellulase for enzymolysis, wherein the quality of collagenase is 0.3% of the quality of fishbone powder %, the mass of cellulase is 0.1% of the mass of fish bone powder, and the enzyme is hydrolyzed for 1.5 hours, during which the temperature is maintained at 25 to 30°C with continuous stirring...

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Abstract

The invention discloses a selenium-enriched sturgeon bone peptide extraction method. The method comprises the steps that (1) a sturgeon bone raw material is smashed and screened through a sieve of 160-300 mesh, and sturgeon bone meal is obtained; (2) the sturgeon bone meal obtained in the step (1) is subjected to primary enzymolysis, sturgeon bone collagen skeletons are degraded, and a coarse enzymolysis product is obtained; (3) the coarse enzymolysis product obtained in the step (2) is subjected to secondary enzymolysis, and a refined enzymolysis product is obtained, so that selenium-enrichedglycoprotein is degraded into micro-molecule polypeptides, wherein the mass proportion of the polypeptides with the molecular weight less than 250 Da exceeds 80%; (4) the refined enzymolysis productobtained in the step (3) is desalted, and a desalted refined enzymolysis product is obtained; (5) after the desalted refined enzymolysis product obtained in the step (4) is subjected to solid and liquid separation, a liquid phase is collected and dried, and the sturgeon bone peptide is obtained. It is shown through an experiment that a selenium-enriched sturgeon bone peptide product is absorbed well and has a significant aging resistance effect.

Description

technical field [0001] The invention belongs to the field of biotechnology, and more specifically relates to an extraction method and product of selenium-enriched sturgeon bone peptide with anti-aging effect. Background technique [0002] Sturgeon is the only living fossil living in water in the world, and it is the fish with the highest nutritional value among all fish. Sturgeon is rich in eight essential amino acids: lysine, leucine, isoleucine, methionine, threonine, tryptophan, phenylaltine and Valine, and as long as the human body lacks one of these eight amino acids, it will cause metabolic disorders. Therefore, the current development of sturgeon products is mainly focused on the absorption and utilization of amino acids, including the protein utilization of sturgeon meat and fish skin tissue. [0003] However, the edible value of sturgeon is not only as a source of amino acids, it is said that sturgeon cartilage and bone marrow (commonly known as "dragon tendons") ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K14/78C07K1/14
CPCC07K1/14C07K14/78C12P21/06
Inventor 黄文樊锋凯林国华林靖杰陈明
Owner HUBEI YANGGUANG SCI & TECHCAL MEDICINES
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