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Lactobacillus plantarum liquid fermentation culture medium and culture method and application thereof

A technology of Lactobacillus plantarum and liquid fermentation, applied in the field of lactic acid bacteria culture and fermentation, can solve the problems of high production cost and complex fermentation technology process, reduce production cost, solve the complex fermentation technology process, and solve the problem of high production cost. Effect

Pending Publication Date: 2019-09-06
XIAMEN HUIYING ANIMAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The object of the present invention is to provide a kind of Lactobacillus plantarum liquid fermentation medium and its culture method, to solve the problems of high production cost and complex fermentation process

Method used

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  • Lactobacillus plantarum liquid fermentation culture medium and culture method and application thereof

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Embodiment 1

[0022] This embodiment is used to illustrate the Lactobacillus plantarum liquid fermentation medium and the culture method thereof provided by the present invention.

[0023] The components of the Lactobacillus plantarum fermentation medium used in this example are: glucose 30g / L, soybean meal powder 12g / L, Saccharomyces cerevisiae extract 10g / L, dipotassium hydrogen phosphate 2g / L, ammonium chloride 10g / L, chloride Sodium 5g / L, calcium carbonate 10g / L, magnesium sulfate 0.2g / L, manganese sulfate 0.05g / L, water 1L, sterilized at 115°C for 30min.

[0024] The fermentation culture method of Lactobacillus plantarum is as follows: use liquid MRS medium as the activation medium, anaerobically culture at 35°C for 18 hours to prepare Lactobacillus plantarum seed liquid, then insert 5% inoculation amount into Lactobacillus plantarum fermentation medium, and then use no Bacterial sodium hydroxide was used to adjust the pH to 7.5, the temperature was controlled at 35°C, anaerobic cultur...

Embodiment 2

[0030] This embodiment is used to illustrate the Lactobacillus plantarum liquid fermentation medium and the culture method thereof provided by the present invention.

[0031] The components of the Lactobacillus plantarum fermentation medium used in this example are: glucose 20g / L, soybean meal powder 10g / L, Saccharomyces cerevisiae extract 10g / L, dipotassium hydrogen phosphate 2g / L, ammonium chloride 5g / L, chloride Sodium 5g / L, calcium carbonate 5g / L, magnesium sulfate 0.2g / L, manganese sulfate 0.05g / L, water 1L, sterilized at 115°C for 30min.

[0032] The fermentation culture method of Lactobacillus plantarum is as follows: use liquid MRS medium as the activation medium, anaerobically culture at 35°C for 18 hours to prepare Lactobacillus plantarum seed liquid, then insert 5% inoculation amount into Lactobacillus plantarum fermentation medium, and then use no Bacterial sodium hydroxide was used to adjust the pH to 7.5, the temperature was controlled at 35°C, anaerobic culture ...

Embodiment 3

[0034] This embodiment is used to illustrate the Lactobacillus plantarum liquid fermentation medium and the culture method thereof provided by the present invention.

[0035] The components of the Lactobacillus plantarum fermentation medium used in this example are: glucose 20g / L, soybean meal powder 15g / L, Saccharomyces cerevisiae extract 8g / L, dipotassium hydrogen phosphate 2g / L, ammonium chloride 8g / L, chloride Sodium 5g / L, calcium carbonate 8g / L, magnesium sulfate 0.2g / L, manganese sulfate 0.05g / L, water 1L, sterilized at 115°C for 30min.

[0036] The fermentation culture method of Lactobacillus plantarum is as follows: use liquid MRS medium as the activation medium, anaerobically culture at 35°C for 18 hours to prepare Lactobacillus plantarum seed liquid, then insert 5% inoculation amount into Lactobacillus plantarum fermentation medium, and then use no Bacterial sodium hydroxide was used to adjust the pH to 7.5, the temperature was controlled at 35°C, anaerobic culture w...

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Abstract

The invention discloses a lactobacillus plantarum liquid fermentation culture medium. The lactobacillus plantarum liquid fermentation culture medium comprises the following components: 20-30 g / L of glucose, 10-15 g / L of soybean meal, 5-10 g / L of a saccharomyces cerevisiae extract, 2-5 g / L of dipotassium phosphate, 5-10 g / L of ammonium chloride, 3-6 g / L of sodium chloride, 5-10 g / L of calcium carbonate, 0.2-0.5 g / L of magnesium sulfate and 0.02-0.05 g / L of manganese sulfate. The invention also discloses a culture method for fermentation of lactobacillus plantarum liquid and application of thefermentation medium in the culture method for fermentation of the lactobacillus plantarum liquid. According to the invention, the glucose monohydrate, the soybean meal, the saccharomyces cerevisiae extract and the ammonium chloride thereof are adopted as raw materials, the composition of the medium is optimized, the technical method of fermentation operation is simplified, the viable count of lactobacillus plantarum is obviously improved, meanwhile, the production cost is reduced, and the production process is simplified.

Description

technical field [0001] The invention belongs to the technical field of culture and fermentation of lactic acid bacteria, and relates to a liquid fermentation medium of Lactobacillus plantarum, a cultivation method thereof, and an application of the fermentation medium. Background technique [0002] Lactobacillus plantarum, a type of lactic acid bacteria, is an anaerobic or facultative anaerobic Gram-positive bacterium. Lactobacillus plantarum acts as a probiotic in the intestinal tract of animals. It can produce natural antibacterial substances such as organic acids and bacteriocins, inhibit the growth of pathogenic bacteria such as E. coli and Salmonella, and improve intestinal health. At the same time, Lactobacillus plantarum can effectively degrade harmful substances such as organic matter in water, ammonia nitrogen and nitrite, and is now widely used in animal feed additives and water environment restoration agents. [0003] The culture of lactic acid bacteria in the la...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/25C12R1/07
CPCC12N1/20
Inventor 林松泉庄若飞乔欣君邹文政林章秀
Owner XIAMEN HUIYING ANIMAL TECH
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