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Method for controlling soil and fixing nitrogen by using blue algae

A cyanobacteria and soil technology, applied in soil conditioning materials, chemical instruments and methods, botanical equipment and methods, etc., can solve the problems of reducing the amount of fertilizer used, unable to fix nitrogen, and destroy nitrogenase, and reduce the amount of fertilizer used. , good nitrogen fixation effect, good effect of loosening soil

Inactive Publication Date: 2019-09-06
山东多芬农业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using the above methods will destroy the cell wall of cyanobacteria, causing the air sacs of cyanobacteria to rupture, thus losing the function of ascending and descending
Applied into the soil, it becomes a dead thing that cannot move. It can only provide organic matter for the soil in terms of conditioning the soil. It cannot loosen the soil through its own movement, and the effect of conditioning the soil is not good.
[0004] Nitrogen-fixing cyanobacteria contain nitrogenase, which can fix nitrogen. However, when the nitrogen-fixing cyanobacteria extract prepared by the above three methods is applied to soil, it can hardly achieve the effect of nitrogen fixation.
The reason is that the applicant believes that the extract prepared by the acid-base chemical method is extracted under acid-base conditions during extraction, and the extraction time is long, which may easily cause enzyme inactivation. Therefore, applying it to the soil cannot achieve nitrogen fixation. The use of microbial fermentation will cause damage to the enzyme, so the effect of nitrogen fixation cannot be achieved when applied to the soil; the use of enzyme extraction, due to the specificity of the enzyme, the selection of the enzyme becomes the key, and the selection of the enzyme is not appropriate. It is also easy to cause damage to nitrogenase, such as protease extraction, because the main component of nitrogenase is protein, so the use of protease extraction will damage the protein in nitrogenase, inactivate the enzyme, and it will not be achieved when applied to the soil. The effect of nitrogen fixation
[0005] At present, there is no product and method for applying nitrogen-fixing cyanobacteria into the soil, which can better loosen the soil, and can better play the role of nitrogenase and reduce the amount of fertilizer used.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The invention relates to a method of using cyanobacteria to treat soil and fix nitrogen, and the cyanobacteria is applied to the soil.

[0031] The cyanobacteria are pulverized Anabaena; the application is basal application.

Embodiment 2

[0033] The invention relates to a method of using cyanobacteria to treat soil and fix nitrogen, and the cyanobacteria is applied to the soil.

[0034] The cyanobacteria is crushed cyanobacteria; the application is base application.

[0035] It also includes applying blue-green algae extract; the blue-green algae in the blue-green algae extract are nitrogen-fixing blue-green algae.

[0036] The cyanobacteria are Anabaena;

[0037] The extraction method of the blue-green algae extract is enzyme extraction; the method of applying the blue-green algae extract is base application.

[0038] The enzymes selected for the enzyme extraction are pectinase and cellulase; the extraction method is carried out according to the following steps:

[0039] 1) Prepare the mixed solution: crush the blue algae and add it to the water to obtain the mixed solution;

[0040] 2) Prepare the crude extract: add pectinase into the mixed solution, extract under certain conditions, inactivate the enzyme,...

Embodiment 3

[0046] The invention relates to a method of using cyanobacteria to treat soil and fix nitrogen, and the cyanobacteria is applied to the soil.

[0047] The cyanobacteria is crushed cyanobacteria; the application is base application.

[0048] It also includes applying blue-green algae extract; the blue-green algae in the blue-green algae extract are nitrogen-fixing blue-green algae.

[0049] The cyanobacteria are Anabaena;

[0050] The extraction method of the blue-green algae extract is enzyme extraction; the method of applying the blue-green algae extract is drip irrigation.

[0051] The enzymes selected for the enzyme extraction are pectinase and cellulase; the extraction method is carried out according to the following steps:

[0052] 1) Prepare the mixed solution: crush the blue algae and add it to the water to obtain the mixed solution;

[0053] 2) Prepare the crude extract: add pectinase into the mixed solution, extract under certain conditions, inactivate the enzyme, ...

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PUM

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Abstract

The invention relates to a method for controlling soil and fixing nitrogen by using blue algae, the method is characterized in that the blue algae are dried and crushed and then applied to the soil, the soil is loosened, and the use amount of fertilizers can be reduced.

Description

technical field [0001] The invention relates to a method for using cyanobacteria to control soil and fix nitrogen. Background technique [0002] cyanobacteria Also known as blue-green algae (blue-green algae), it is a class of long evolutionary history, negative Gram staining, no flagella, containing chlorophyll a, but does not contain chloroplasts (different from eukaryotic algae), capable of oxygen production Large photosynthetic unicellular prokaryotes. Some cyanobacteria cause eutrophic seawater "green tide" and lake "bloom" after being polluted by nitrogen, phosphorus and other elements, which bring serious harm to fishery and aquaculture. The usual treatment is to landfill or incinerate the blue-green algae after salvage. Because cyanobacteria contain toxins such as cyclic peptides and alkaloids, the way of landfilling may cause pollution to the water body; the method of burning will cause pollution to the atmosphere. [0003] At present, there are also methods of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K17/14A01N65/00A01N63/00A01P21/00C09K101/00
CPCA01N63/00A01N65/00C09K17/14C09K2101/00
Inventor 赵广林钟美娟范孝芹
Owner 山东多芬农业有限公司
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