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Method for evaluating biological damage caused by low-dose gamma ray irradiation by utilizing isocitrate dehydrogenase 2

A technology of isocitrate dehydrogenase and gamma rays, which can be used in biological testing, material inspection products, etc., and can solve problems such as changes in cell components and structures, and biological damage

Inactive Publication Date: 2019-09-03
NANHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Gamma ray irradiation can cause significant changes in cell composition and structure, and cause serious damage to organisms

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] C57BL / 6J adult male mice aged 6 to 7 weeks were selected and randomly divided into 4 groups, with 6 mice in each group. The animals were reared in an environment with a radiation dose rate of 12.5 μGy / h, the total irradiation time was 90 d, and the irradiation was performed for 8 h per day. The eyeball method was used to collect blood, and the collected blood was placed in 4 o The serum was separated by centrifugation at 3000 r / min for 20 min the next day. The collected serum of C57BL / 6J mice irradiated with low-dose ionizing rays was subjected to protein extraction, total protein concentration determination, protein denaturation, gel electrophoresis, membrane transfer, immunoreaction, chemiluminescence reaction, gel imaging scanning analysis, Finally, according to formula (1), the relative expression down-regulation rate of IDH2 was analyzed.

[0043] C57BL / 6J adult male mice were irradiated at a dose rate of 12.5 μGy / h for 90 d, irradiated for 8 h a day, and irradia...

Embodiment 2

[0045] C57BL / 6J adult male mice aged 6 to 7 weeks were selected and randomly divided into 4 groups, with 6 mice in each group. The animals were reared in an environment with a radiation dose rate of 25.0 μGy / h, the total irradiation time was 90 d, and the irradiation was performed for 8 h per day. The eyeball method was used to collect blood, and the collected blood was placed in 4 o The serum was separated by centrifugation at 3000 r / min for 20 min the next day. The collected serum of C57BL / 6J mice irradiated with low-dose ionizing rays was subjected to protein extraction, total protein concentration determination, protein denaturation, gel electrophoresis, membrane transfer, immunoreaction, chemiluminescence reaction, gel imaging scanning analysis, Finally, according to formula (1), the relative expression down-regulation rate of IDH2 was analyzed.

[0046] C57BL / 6J adult male mice were irradiated at a dose rate of 25.0 μGy / h for 90 d, 8 h a day, and 18 mGy low-dose gamma ...

Embodiment 3

[0048] C57BL / 6J adult male mice aged 6 to 7 weeks were selected and randomly divided into 4 groups, with 6 mice in each group. The animals were reared in an environment with a radiation dose rate of 50.0 μGy / h, the total irradiation time was 90 d, and the irradiation was performed for 8 h per day. The eyeball method was used to collect blood, and the collected blood was placed in 4 o The serum was separated by centrifugation at 3000 r / min for 20 min the next day. The collected serum of C57BL / 6J mice irradiated with low-dose ionizing rays was subjected to protein extraction, total protein concentration determination, protein denaturation, gel electrophoresis, membrane transfer, immunoreaction, chemiluminescence reaction, gel imaging scanning analysis, Finally, according to formula (1), the relative expression down-regulation rate of IDH2 was analyzed.

[0049] C57BL / 6J adult male mice were irradiated at a dose rate of 50.0 μGy / h for 90 d, 8 h a day, and irradiated with a low ...

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Abstract

The invention relates to a method for evaluating biological damage caused by low-dose gamma ray irradiation by utilizing isocitrate dehydrogenase 2. According to the dose-effect relationship between the irradiation dose of low-dose gamma rays received by a C57BL / 6J mouse and the down-regulation rate of the relative expression quantity of IDH2 in serum, the down-regulation rate of the relative expression level of IDH2 in the serum of a C57BL / 6J mouse, biological damage from low-dose gamma radiation was assessed. On the basis of establishing a low dose rate irradiation C57BL / 6J mouse model, significant differential expression protein is screened out by using an iTRAQ technology and is verified by using Western blot. The method for evaluating biological damage caused by low-dose gamma ray irradiation by utilizing isocitrate dehydrogenase 2 has the advantages of sensitivity to low-dose radiation, high reliability, strong specificity and the like.

Description

technical field [0001] The invention belongs to the technical field of biological damage evaluation of low-dose gamma-ray irradiation, and uses molecular markers to evaluate the biological damage of low-dose gamma-ray irradiation, specifically using isocitrate dehydrogenase in serum of C57BL / 6J mice 2 (Isocitrate dehydrogenase 2 , IDH2) relative expression down-regulation rate to evaluate the method of low-dose gamma ray irradiation biological damage. Background technique [0002] "Nuclear pollution" refers to the process of natural radioactive nuclear isotopes and artificially synthesized nuclear isotopes spreading in the environment. It includes not only the pollution caused by nuclear radiation and atomic dust itself, but also the secondary pollution caused by the pollution of these substances to the environment. Gamma ray irradiation can cause significant changes in cell components and structures, and cause serious damage to organisms. High doses of ionizing radiation ...

Claims

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Application Information

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IPC IPC(8): G01N33/49
CPCG01N33/49
Inventor 丁德馨胡南易岚龙鼎新赵维超殷杰穆红香李广悦
Owner NANHUA UNIV
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