Construction and identification methods of infectious clones of porcine circovirus type 3

Abstract

The invention discloses a construction method and identification method of infectious clones of porcine circovirus type 3 (PCV3). During construction, the characteristic of a circovirus itself, namely, a characteristic that the nucleic acid of the PCV3 is cyclic DNA and any position can be regarded as a starting point or an end point is used. Firstly, a DNA sequence of the PCV3 is rearranged and then transformed. In previous studies, the PCV3 virus is identified only according to clinical tissues, and Western blot identification standards for pathogen detection are not reported still. Therefore, based on preparation of the PCV3 Cap monoclonal antibodies, infectious clones are constructed and identified. The method does not use foreign plasmids to obtain the infectious clones of the PCV3, and thus the infection of foreign genes is eliminated; a unique detection primer is designed, can only detect cyclic PCV3 DNA by PCR, and cannot detect PCV3 DNA before cyclization by PCR. The specificmonoclonal antibodies of the PCV3 are used for detection in PCR detection, and experimental data are more credible.

Description

technical field [0001] The invention belongs to the field of microbiology, and specifically relates to obtaining the pathogen of porcine circovirus type 3 (PCV3) by artificial means, and discloses a method for constructing and identifying infectious clones of porcine circovirus type 3. Background technique [0002] Porcine circovirus (PCV) has long threatened the healthy development of my country's pig industry. Previous studies mainly focused on porcine circovirus type 2 (PCV2). In recent years, the emerging porcine circovirus type 3 (PCV3) has caused huge economic losses to the pig industry. However, due to the different growth characteristics and infected cell lines between PCV3 and PCV2, the current research on PCV3 is limited to the field of clinical disease detection. In order to better prevent and control PCV3 infection, it is imminent to obtain PCV3 pathogens. [0003] The traditional method of constructing infectious clones is to construct the linear DNA of the v...

AI Technical Summary

Problems solved by technology

In recent years, the emerging porcine circovirus type 3 (PCV3) has caused huge economic losses to the pig industry

However, due to the different growth characteristics and infected cell lines of PCV3 and PCV2, the current research on PCV3 is limited to the field of clinical disease detection.

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