A kind of mushroom strain and breeding method thereof
A technology of jiji mushrooms and strains, applied in the field of edible fungus breeding, can solve the problem of single strains and achieve the effect of improving economic benefits
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[0040] A new variety of edible fungus jijiji mushroom is obtained by crossing the following methods:
[0041] (1) Remove the caps of the uncapped seafood mushroom strains and crab-flavored mushrooms respectively, and place them in a sterile empty petri dish, so that the caps can autonomously eject the spores to the surface of the empty petri dish, and then collect them with 1 mL of distilled water. 2 mL PE tube;
[0042] (2) Under sterile conditions, dilute the spores collected in step (1) until 2-3 spores are observed under a 40-fold microscope, and then spread and culture on PDA medium. When awn-like single colonies appear At the same time, pick the tip hyphae of a single colony and culture it in a flat PDA medium, and cultivate it at a constant temperature of 25°C for 150h;
[0043] (3) Under sterile conditions, draw 7uL of lactic acid carbolic acid cotton blue staining solution on the glass slide, pick the hyphae cultured in step (2) and place them in the staining solutio...
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